Resistance of oxidative stress in biofilm and planktonic cells

Jakubowski, Witold; Walkowiak, Bogdan

doi:10.1590/s1516-8913201500356

Cited by 14 publications

(7 citation statements)

References 35 publications

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“…Furthermore, it is reported that cells use ROS as a signal or cue to adapt to a changing environment ( Čáp et al, 2012 ). Indeed, a connection between biofilm formation and oxidative stress has already been observed ( Kaplan, 2011 ; Čáp et al, 2012 ; Villa et al, 2012 ; Cattò et al, 2015 , 2017 ; Jakubowski and Walkowiak, 2015 ; Gambino and Cappitelli, 2016 ).…”

Section: Discussionmentioning

confidence: 92%

Effects of Sub-lethal Concentrations of Silver Nanoparticles on a Simulated Intestinal Prokaryotic–Eukaryotic Interface

et al. 2018

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Nanotechnology applications are expected to bring a range of benefits to the food sector, aiming to provide better quality and conservation. In this research, the physiological response of both an Escherichia coli mono-species biofilm and Caco-2 intestinal cells to sub-lethal concentrations of silver nanoparticles (AgNPs) has been investigated. In order to simulate the anaerobic and aerobic compartments required for bacteria and intestinal cells growth, a simplified semi-batch model based on a transwell permeable support was developed. Interaction between the two compartments was obtained by exposing Caco-2 intestinal cells to the metabolites secreted by E. coli biofilm after its exposure to AgNPs. To the best of the authors' knowledge, this study is the first to investigate the effect of AgNPs on Caco-2 cells that takes into consideration previous AgNP-intestinal biofilm interactions, and at concentrations mimicking real human exposure. Our data show that 1 µg/mL AgNPs in anaerobic conditions (i) promote biofilm formation up to 2.3 ± 0.3 fold in the first 72 h of treatment; (ii) increase reactive oxygen species (ROS) production to 84 ± 21% and change the physiological status of microbial cells after 96 h of treatment; (iii) seriously affect a 72-h old established biofilm, increasing the level of oxidative stress to 86 ± 21%. Moreover, the results indicate that oxygen renders the biofilm more adequate to counteract AgNP effects. Comet assays on Caco-2 cells demonstrated a protective role of biofilm against the genotoxic effect of 1 µg/mL AgNPs on intestinal epithelial cells.

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Section: Discussionmentioning

confidence: 92%

Effects of Sub-lethal Concentrations of Silver Nanoparticles on a Simulated Intestinal Prokaryotic–Eukaryotic Interface

et al. 2018

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“…An alternative explanation for the relatively increased eDNA release by S. epidermidis biofilm cells could be related to the high production of catalase enzyme [ 56 ] and ECM [ 12 , 57 ] which neutralizes and reacts with the H 2 O 2 respectively. It has also been shown that NaOCl reacts with the organic components of the ECM thereby reducing its concentration [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Susceptibility patterns and the role of extracellular DNA in Staphylococcus epidermidis biofilm resistance to physico-chemical stress exposure

et al. 2018

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BackgroundOver 65% of human infections are ascribed to bacterial biofilms that are often highly resistant to antibiotics and host immunity. Staphylococcus epidermidis is the predominant cause of recurrent nosocomial and biofilm-related infections. However, the susceptibility patterns of S. epidermidis biofilms to physico-chemical stress induced by commonly recommended disinfectants [(heat, sodium chloride (NaCl), sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2)] in domestic and human healthcare settings remains largely unknown. Further, the molecular mechanisms of bacterial biofilms resistance to the physico-chemical stresses remain unclear. Growing evidence demonstrates that extracellular DNA (eDNA) protects bacterial biofilms against antibiotics. However, the role of eDNA as a potential mechanism underlying S. epidermidis biofilms resistance to physico-chemical stress exposure is yet to be understood. Therefore, this study aimed to evaluate the susceptibility patterns of and eDNA release by S. epidermidis biofilm and planktonic cells to physico-chemical stress exposure.ResultsS. epidermidis biofilms exposed to physico-chemical stress conditions commonly recommended for disinfection [heat (60 °C), 1.72 M NaCl, solution containing 150 μL of waterguard (0.178 M NaOCl) in 1 L of water or 1.77 M H2O2] for 30 and 60 min exhibited lower log reductions of CFU/mL than the corresponding planktonic cells (p < 0.0001). The eDNA released by sub-lethal heat (50 °C)-treated S. epidermidis biofilm and planktonic cells was not statistically different (p = 0.8501). However, 50 °C-treated S. epidermidis biofilm cells released significantly increased eDNA than the untreated controls (p = 0.0098). The eDNA released by 0.8 M NaCl-treated S. epidermidis biofilm and planktonic cells was not significantly different (p = 0.9697). Conversely, 5 mM NaOCl-treated S. epidermidis biofilms exhibited significantly increased eDNA release than the corresponding planktonic cells (p = 0.0015). Further, the 50 μM H2O2-treated S. epidermidis biofilms released significantly more eDNA than the corresponding planktonic cells (p = 0.021).ConclusionsS. epidermidis biofilms were less susceptible to physico-chemical stress induced by the four commonly recommended disinfectants than the analogous planktonic cells. Further, S. epidermidis biofilms enhanced eDNA release in response to the sub-lethal heat and oxidative stress exposure than the corresponding planktonic cells suggesting a role of eDNA in biofilms resistance to the physico-chemical stresses.

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“…The bacterial biofilms are more difficult to remove compared to planktonic bacterial cells. [34][35] Therefore, the evaluation of biofilm formation inhibition activity was also conducted in this study. DLBS1355 o/w emulsion, which contains 0.5% N. sativa seed oil extract, effectively inhibited biofilm formation of S. mutans, S. sanguis and mixed culture of S. mutans and S. sanguis.…”

Section: Discussionmentioning

confidence: 99%

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2017

IJPSR

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Our previous study highlighted the antimicrobial activity evaluation of Nigella sativa seed oil extract that was obtained from supercritical fluid extraction using carbon dioxide (SCFE-CO 2 ) on Gram positive pathogenic bacteria. The aim of this study was to formulate oil-in-water (o/w) emulsion of previous N. sativa seed oil extract and to investigate its antimicrobial activity against Streptococcus mutans and Streptococcus sanguis as the most common causative bacteria of dental caries. Oil-in-water emulsion containing 10% N. sativa seed oil extract (DLBS1355) exhibited good inhibition activity on both S. mutans and S. sanguis. Time-kill assay and biofilm inhibition assay were conducted using emulsion with 0.5% N. sativa seed oil extract content. The results showed bactericidal and biofilm inhibition activities on both tested bacteria. Antimicrobial activity of the emulsion was found higher on S. sanguis than S. mutans. Results of the present study showed that emulsification process did not significantly affect the antimicrobial activity of N. sativa seed oil extract and the formulated N. sativa oil-in-water emulsion could further be used as an antimicrobial agent against dental cariogenic bacteria.

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Resistance of oxidative stress in biofilm and planktonic cells

Abstract: ABSTRACT

Cited by 14 publications

References 35 publications

Effects of Sub-lethal Concentrations of Silver Nanoparticles on a Simulated Intestinal Prokaryotic–Eukaryotic Interface

Effects of Sub-lethal Concentrations of Silver Nanoparticles on a Simulated Intestinal Prokaryotic–Eukaryotic Interface

Susceptibility patterns and the role of extracellular DNA in Staphylococcus epidermidis biofilm resistance to physico-chemical stress exposure

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