Production of Polyhydroxybutyrate by Bacillus axaraqunsis BIPC01 using Petrochemical Wastewater as Carbon Source

Mayeli, Nasim; Motamedi, Hossein; Heidarizadeh, Fariba

doi:10.1590/s1516-8913201500048

Cited by 19 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, much effort has been committed to reducing the production cost of PHBs by using low-cost carbon sources [9]. Bacillus axaraqunsis BIPC01, which was isolated from Bandar Imam, Iran, could be a potent PHB producer using petrochemical wastewater as a carbon source [10].…”

Section: Introductionmentioning

confidence: 99%

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

et al. 2021

View full text Add to dashboard Cite

Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their ability to accumulate PHB granules inside their cells. Isolate AS-02 was isolated from cattle manure and identified as Bacillus wiedmannii AS-02 OK576278 by means of 16S rRNA analysis. It was found to be the best producer. The optimum pH, temperature, and incubation period for the best PHB production by the isolate were 7, 35 °C, and 72 h respectively. PHB production was the best with peptone and glucose as nitrogen and carbon sources at a C/N ratio of (2:1). The strain was able to accumulate 423, 390, 249, 158, and 144 mg/L PHB when pretreated orange, mango, banana, onion peels, and rice straw were used as carbon sources, respectively. The extracted polymer was characterized by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. The isolate B. wiedmannii AS-02 OK576278 can be considered an excellent candidate for industrial production of PHB from agricultural wastes.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…They found that when C:N ratio was raised from 24 to 144 the polymer yield was increased to a maximum of 33% of biomass (w/w). Mayeli et al, 2015 used petrochemical wastewater as the source of carbon for the production of polyhydroxyalkanoates (PHA) in an effort to decrease its cost of production. Getachew & Woldesenbet, (2016) isolated Bacillus sp.…”

Section: Cost Effective and Expeditious Phb Production Protocol-need mentioning

confidence: 99%

Recent updates on the economic use of poly hydroxy butyrate (PHB): A green alternative to plastics

Jangra¹,

Ikbal²,

Pippal³

et al. 2018

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

Excessive uses of polymer plastics in boundless spheres have resulted in waste accumulation. There is an urgent need for switching over to compostable and biodegradable plastics that too from renewable resources. Polyhydroxybutyrate (PHB) is a unique biopolymer which is biodegradable and most close to plastics which is miscible with the conventional plastic. The adaptation and commercialization of PHB depend upon its ready availability in a pocketfriendly manner. The following presentation attempts to discuss economic strategies to reduce the production costs of PHB as well as its expeditious production from Agri-byproducts and its applications in various fi elds. Various sources like microorganisms, genetically modifi ed bacteria, plants and different methods are being looked into, so that quality, quantity and fi nancial side of PHB production can be controlled. It is presented here that PHB production is a natural capability of microbes exploited recently. This potential in the form of new technology is contributing to fulfi l the need of plastic along with the environmental beauty and cleanliness. Novel bacterial fermentation strategies of polyhydroxybutyrate production, for improved productivity and quality of PHB for commercial production are summarized here.

show abstract

“…From the representative spectrum (Figs. 2a and 2b), the multiplet peaks at 2.5 ppm and triplet peaks at 5.2 ppm were assigned to methylene and methine protons of the α-and β-carbon in both 3HB and 3-hydroxyalkanoates (3HAs) copolymers, respectively [28,29]. The chemical shift at 0.8 and 1.2 ppm were assigned to the terminal methyl protons of the 3HB and 3HA polymers, respectively [8].…”

Section: Pha Characterizationmentioning

confidence: 99%

Unusual poly(3‐hydroxyalkanoate) (PHA) biosynthesis behavior of Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002 isolated from palm oil mill effluent

Razaif-Mazinah

Anis

Harun

et al. 2016

Biotech and App Biochem

View full text Add to dashboard Cite

Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002, isolated from palm oil mill effluent, accumulated poly(3-hydroxyalkanoates) (PHAs) when grown on aliphatic fatty acids, sugars, and glycerol. The substrates were supplied at 20:1 C/N mole ratio. Among C-even n-alkanoic acids, myristic acid gave the highest PHA content 26 and 28 wt% in P. putida and D. tsuruhatensis, respectively. Among C-odd n-alkanoic acids, undecanoic gave the highest PHA content at 40 wt% in P. putida and 46 wt% in D. tsuruhatensis on pentadecanoic acid. Sugar and glycerol gave <10 wt% of PHA content for both bacteria. Interestingly, D. tsuruhatensis accumulated both short- and medium-chain length PHA when supplied with n-alkanoic acids ranging from octanoic to lauric, sucrose, and glycerol with 3-hydroxybutyrate as the major monomer unit. In P. putida, the major hydroxyalkanoates unit was 3-hydroxyoctanoate and 3-hydroxydecanoate when grown on C-even acids. Conversely, 3-hydroxyheptanoate, 3-hydrxoynonanoate, and 3-hydroxyundecanoate were accumulated with C-odd acids. Weight-averaged molecular weight (M ) was in the range of 53-81 kDa and 107-415 kDa for P. putida and D. tsuruhatensis, respectively. Calorimetric analyses indicated that both bacteria synthesized semicrystalline polymer with good thermal stability with degradation temperature (T ) ranging from 178 to 282 °C.

show abstract

Production of Polyhydroxybutyrate by Bacillus axaraqunsis BIPC01 using Petrochemical Wastewater as Carbon Source

Abstract: The aim of this study was to use petrochemical wastewater as the source of carbon for the production of polyhydroxyalkanoates (PHA)

Cited by 19 publications

References 19 publications

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

Recent updates on the economic use of poly hydroxy butyrate (PHB): A green alternative to plastics

Unusual poly(3‐hydroxyalkanoate) (PHA) biosynthesis behavior of Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002 isolated from palm oil mill effluent

Contact Info

Product

Resources

About