Commercial Biological Control Agents Targeted Against Plant-Parasitic Root-knot Nematodes

Tranier, Marie-Stéphane; Pognant-Gros, Johan; Quiroz, Reynaldo de la Cruz; González, Cristóbal; Mateille, Thierry; Roussos, Sévastianos

doi:10.1590/s1516-8913201402540

Cited by 55 publications

(26 citation statements)

References 42 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Conidia and chlamydospores of P. chlamydosporia and conidia of P. lilacinum were counted in a Neubauer chamber and suspension diluted to a final concentration of 1 3 10 6 spores/ml. In vitro parasitism of M. enterolobii eggs by strains of P. chlamydosporia and P. lilacinum: Nineteen strains of P. chlamydosporia and 14 strains of P. lilacinum were tested against M. enterolobii eggs, including the active ingredient of the commercial products KlamiC (Centro Nacional de Sanidad Agropecuaria, La Habana, Cuba; Hern andez and Hidalgo-Diaz, 2008) and Biostat WP (Laverlam S.A., Cali, Colombia; Tranier et al, 2014). The bioassays were performed in 12-well plates and each well was filled with 3 ml of water agar (1.5% v/v).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Silva

Carneiro

Faria

et al. 2017

Journal of Nematology

View full text Add to dashboard Cite

Meloidogyne enterolobii is one of the most important root-knot nematode in tropical regions, due to its ability to overcome resistance mechanisms of a number of host plants. The lack of new and safe active ingredients against this nematode has restricted control alternatives for growers. Egg-parasitic fungi have been considered as potential candidates for the development of bionematicides. In tissue culture plates, Pochonia chlamydosporia (var. catenulata and chlamydosporia) and Purpureocillium lilacinum strains were screened for their ability to infect eggs of the root-knot nematode M. enterolobii on water-agar surfaces. Reduction in the hatching of J2 varied from 13% to 84%, depending on strain. The more efficacious strains reduced hatchability of J2 by 57% to 84% when compared to untreated eggs, but average reductions were only 37% to 55% when the same strains were applied to egg masses. Combinations of fungal isolates (one of each species) did not increase the control efficacy in vitro. In experiments in which 10,000 nematode eggs were inoculated per plant, reductions in the number of eggs after 12 months were seen in three of four treatments in banana plants, reaching 34% for P. chlamydosporia var. catenulata. No significant reductions were seen in tomato plants after 3 mon. In another experiment with tomato plants using either P. chlamydosporia var. catenulata or P. lilacinum, the number of eggs was reduced by 34% and 44%, respectively, when initial infestation level was low (500 nematode eggs per plant), but tested strains were not effective under a moderate infestation level (5,000 eggs per plant). Under all infestation levels tested in this work, gall and egg mass indexes (MI) did not differ from the untreated controls, bringing concerns related to the practical adoption of this control strategy by farmers. In our opinion, if the fungi P. chlamydosporia and P. lilacinum are to be used as biocontrol tools toward M. entorolobii, they should focus on agricultural settings with low soil infestation levels and within an IPM approach.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Silva

Carneiro

Faria

et al. 2017

Journal of Nematology

View full text Add to dashboard Cite

show abstract

“…Biological control is a natural process occurring in all habitats, nevertheless it can be introduced in agricultural areas by using a living organism (Tranier et al, 2014) to keep low a population level of another organism (Agbenin, 2011). Amongst several potential agents, fungi have been traditionally studied (Vega et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…They are filamentous endoparasites that adhere nematodes to a trapping apparatus to immobilize them and penetrate their cuticle, invading the body and digesting its nutrients (Tranier et al, 2014). Barron (2003) observed ligninases and cellulases production, suggesting that Hohenbuehelia (syn.…”

Section: Introductionmentioning

confidence: 99%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

View full text Add to dashboard Cite

Biological control is a method of controlling pests through the use of other living organisms. The purposes of this study were to test Hohenbuehelia species as biological control agents against Panagrellus redivivus in vitro, evaluating nematodes influence on mycelia growth; establishing daily indexes for predation and growth and setting predation percentage. Five species previously identified as 436-Hohenbuehelia mastrucata (Nematoctonus hamatus), 528-H. bullulifera (not described so far), 581-H. paraguayensis (N. sp.), 582-H. sp. (N. sp.) and 631-H. portegna (N. campylosporus) were submitted to anamorphic purification directly from basidioma. Afterwards, 100 nematodes were added to each pure colony for predation test. Evaluation started right after 24 hours of nematode-fungus interaction. Immobilized and/or penetrated nematodes were counted and mycelia growth was measured. Results were subjected to variance analyses. Hohenbuehelia mastrucata had the best performance in growth speed, followed by H. portegna and H. paraguayensis; Nematodes multiplyied much but none specie grew more as an influence of their movement under mycelium, however all species formed trap devices and some of them produced adhesive or repelent substances. Trap devices were formed in control plates also. The plates of H. paraguayensis without nematodes grew more than treatments. Cumulative predation of H. portegna was the highest at 24 (195.5%) and 48 hours (235%). At the last evaluation day, H. paraguayensis preyed the same amount (185.75%) than H. portegna, followed by H. mastrucata (109.51%). Resulst of predation daily indexes displayed chronological activity for each isolate, where H. portegna was very reactive at first 24 hours, H. mastrucata raised its predacious activity in 48 hours being constant from this time on and H. paraguayensis pointed out itself at 72 hours. Other species presented low predation and growth indexes throughout experiment.

show abstract

“…Similarly to P. chlamydosporia, P. lilacinus is a parasite of eggs, females and cysts, being one of the most studied hyphomycetes to biological control use at plant parasitic nematodes, presenting low specificity to the host and some differences of effectiveness depending of the strain (Atkins et al 2005;Medeiros et al 2018). Despite its classification, it presents the ability of antagonizes all the nematodes stages, although it is especially effective against eggs (Tranier et al 2014). P. lilacinus is primarily saprophyte, living in different substrates of the soil (Ferraz et al 2012), and eventually colonizes the eggs, through direct penetration of the egg shell by an individual hyphae.…”

Section: Paecilomyces Lilacinusmentioning

confidence: 99%

“…There are other resistance inducers that have already showed similar effects against several phytonematodes in several crops, such as potassium phosphide (Oka et al 2007;Dias-Arieira et al 2012); the β-amynobutyric (Oka et al 1999;Sahebani and Hadavi 2009;Chinnasri et al 2006), several rhizobacteria (Siddiqui and Shaukat 2002a;Siddiqui and Shaukat 2002b); Trichoderma spp. (Tranier et al 2014), and arbuscular mycorrhizic fungus (Vos et al 2012;Daneshkah et al 2013).…”

Section: Resistance Inducers: Acibenzolar-s-methyl Thaxtomin a And Fmentioning

confidence: 99%

Meloidogyne incognita and melon plants: host status, induced resistance and biological control

Souza¹

View full text Add to dashboard Cite

Commercial Biological Control Agents Targeted Against Plant-Parasitic Root-knot Nematodes

Abstract: ABSTRACT

Cited by 55 publications

References 42 publications

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Evaluation of Pochonia chlamydosporia and Purpureocillium lilacinum for Suppression of Meloidogyne enterolobii on Tomato and Banana

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Meloidogyne incognita and melon plants: host status, induced resistance and biological control

Contact Info

Product

Resources

About