Validation of computationally predicted substrates for laccase

Reena, .; Dhall, Purnima; Kumar, Ram; Kumar, Anil

doi:10.1590/s1516-8913201402239

Cited by 11 publications

(2 citation statements)

References 33 publications

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“…Resorcinol is a non-expensive catechol isomer that has already been used as a substrate to produce colored homopolymers and heteropolymers via laccase-catalyzed oxidation and has also been exploited in laccase-catalyzed fiber dyeing ( Durairaj, 2005 ; Sun et al, 2013 ; Reena et al, 2014 ; Sýs et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

In Situ Wood Fiber Dyeing Through Laccase Catalysis for Fiberboard Production

Colella¹,

Chiaro²,

Lettera³

2021

Front. Bioeng. Biotechnol.

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The aim of the present work was to develop an innovative and environmentally friendly process for wood fiber dyeing and to produce 3-dimensionally fully colored medium-density fiberboard (MDF). The potential of laccase-catalyzed polymerization of selected precursors to form dyes useful in fiberboard manufacturing, a technique used for the first time in this field, was demonstrated. Some of the 7 aromatic compounds tested yielded colored products after laccase treatment under both acid and alkaline conditions, and a good variety of colors was attained by using mixtures of two different monomers. To demonstrate the coloration and design potential of laccase conversion of aromatic compounds, MDFs were enzymatically dyed using an in situ one-step laccase-catalyzed coloration process, and the results were compared against commercial MDFs obtained by using organic coloring agents. Important advantages over conventional processing methods include good color fastness and, in some cases, new hydrophobic properties, allowing designers and woodworkers to explore the beauty of textures and the use of simpler and milder processing conditions that eliminate harsh chemical use and reduce energy consumption.

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Section: Discussionmentioning

confidence: 99%

In Situ Wood Fiber Dyeing Through Laccase Catalysis for Fiberboard Production

Colella¹,

Chiaro²,

Lettera³

2021

Front. Bioeng. Biotechnol.

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“…The in-silico approach can predict chemical nature of contaminant, novel xenobiotics biodegradation pathways and microorganisms capable of biotransformation at cellular and environmental level [14]. A better remediation approach uniting theoretical in-silico techniques followed by experimental confirmation is suggested as a time and cost-effective method since a manifold increase in xenobiotics make it impractical to screen them all experimentally for bioremediation [15].…”

Section: Introductionmentioning

confidence: 99%

In Silico Analysis of Bacterial Systems For Textile Azo Dye Decolorization and Affirmation With Wetlab Studies

Srinivasan

Shanmugam²,

Surwase

et al. 2017

CLEAN Soil Air Water

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A systemic approach involving in‐silico and in‐vitro tests were used for the decolorization of five azo dyes, Reactive Yellow F3R, Joyfix Yellow 53R, Remazol Red RR, Drimaren Black CL‐S and Disperse Red F3BS, using Aeromonas hydrophila SK16 and Lysinibacillus sphaericus SK13. Homology models for laccase and azoreductase enzymes from these two bacteria were generated using Accelrys Discovery Studio 3.5 and validated. Docking was performed with LeadIT software for binding energy calculation, and active site residues were identified. Reactive Yellow F3R showed highest binding energy with A. hydrophila, which emulated the decolorization percentage by ultraviolet–visible (UV–vis) spectroscopy. High‐performance LC (HPLC), Fourier transform IR spectroscopy (FTIR), and gas chromatography–mass spectrometry (GCMS) supported biotransformation and biodegradation of Reactive Yellow F3R by A. hydrophila SK16, and a biodegradation pathway was proposed. HPLC analysis of treated sample illustrated peaks at retention time 1.481, 3.165, 3.374, and 3.945 min while the control dye showed peaks at 1.478 and 3.106 min. GCMS analysis showed that (2Z)‐but‐2‐ene, 1,3,5‐triazine, aniline, and naphthalene were formed as end products. The in‐silico outcome was in good agreement with experimental studies. Thus, it can be surmised that in‐silico molecular modeling and docking studies can assist as a preliminary tool for screening of potential bacterial system to be employed in textile dye decolorization and degradation studies.

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