Production of Beauveria bassiana Air Conidia by Means of Optimization of Biphasic System Technology

Gouli, Vladimir V.; Gouli, Svetlana Y.

doi:10.1590/s1516-8913201401745

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Although rice and barley seem to be the major substrates used in the tropics and the Northern Hemisphere respectively [5], there has been considerable effort to identify low-cost agricultural materials, especially byproducts and waste products, as suitable substrates for mass production have taken by numerous researches from different countries (Table 1). There are different methods of mass production like, solid state fermentation [6,7], liquid fermentation, submerged stat fermentation and biphasic culture system [8,9] based on the type substrates, out of all, solid state fermentation has emerged as an appropriate technology.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Ranadev

Nagaraju

Kumari

et al. 2023

CJAST

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Aim: The study was undertaken to evaluate agro-wastes (Post mushroom substrate, Sugarcane bagasse, rice husk and sorghum grains) for mass production of entomopathogenic fungi like, I. fumosoroseus:MT997932, B. bassiana: MT997933, L. lecanii: MT997935 and H. thompsonii: MT997936 by solid stat fermentation. Place and Duration of Work: The study was carried out in the Department of Agricultural Microbiology, GKVK, University of Agricultural Sciences, Bangalore during 2019-20. Methodology: The substrates were dried, chopped and sieved through 2mm sieve. 100g of all substrates were sterilized and moistened to 60% by adding sterile distilled water fallowed by addition of 5ml of spore suspension of fungal isolates. Treatments T5. T6, T7, T8 and T10 were fortified with adding 10ml of molasses. Observation like, growth and spore production were taken at 7, 14 and 21 days after inoculation. Results: The growth and spore production of entomopathogenic fungal isolates were observed to be increased accordance with the incubation period. Among four substrates maximum mycelial growth and spore production of all the isolates (×109 spores g-1) was observed in sorghum grains (T4:T8) fallowed by treatment T7 (SMS+10% molasses) on 21 DAI. Whereas, sugarcane bagasse and paddy husk substrates were not supported the satisfactory growth and spore production besides the addition of molasses (×107 spores g-1). The addition of molasses has positively influenced the growth and spore production of entomogenus isolates in all treatments. Conclusion: Based on results, it is evidenced that even though cereal grains are best option for mass production, PMS fortified with molasses will become a better substrate for mass production and reduce the load of using food grains as substrate.

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Section: Introductionmentioning

confidence: 99%

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Ranadev

Nagaraju

Kumari

et al. 2023

CJAST

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Technology for the production of microbial pesticides

Gouli¹,

Marcelino²,

Gouli³

2021

Microbial Pesticides

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Biological control of Western flower thrips, Frankliniella occidentalis using a self-sustaining granular fungal treatment

Davari

Parker

Sullivan

et al. 2021

Bull. Entomol. Res.

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Western flower thrips (WFT), Frankliniella occidentalis, is one of the most destructive pests of vegetables, fruits and ornamental crops worldwide, causing extensive damage by direct feeding of the crop and transmitting economically important viruses. Despite the successes of biocontrol agents to control WFT, more efficient and cost-effective ways must be found to encourage grower adoption of integrated pest management. A sustainable fungal treatment was developed to preserve fungal inoculum in potting soil and reduce thrips populations. Combining cooked, oven-dried millet with BotaniGard® (a commercial form of Beauveria bassiana strain GHA) to potting soil increased spore production and persistence of the fungus in the soil. In treated pots with millet, spore concentrations were 3–4 times greater after 30 days compared with spore yields at 10 days. The number of WFT adults was significantly lower in the marigold pots treated with GHA mix + millet than untreated controls, 12% and 10% in treated pots and 70% and 68% in untreated pots in sterile and non-sterile soil, respectively. Incorporation of millet in the potting mix enhanced the effect of the fungal treatments by providing a nutritive substrate on which the fungus could become established. This method is relatively inexpensive and easy for growers to use in greenhouses because granular formulations of B. bassiana are not commercially available.

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Production of Beauveria bassiana Air Conidia by Means of Optimization of Biphasic System Technology

Cited by 4 publications

References 8 publications

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Evaluating the Stability of Post Mushroom Substrate (PMS) and Other Agro-wastes for Mass Production of Entomopathogenic Fungi

Technology for the production of microbial pesticides

Biological control of Western flower thrips, Frankliniella occidentalis using a self-sustaining granular fungal treatment

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