Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology

Stergiou, Panagiota‐Yiolanda; Foukis, Athanasios; Theodorou, Leonidas G.; Papagianni, Maria; Papamichael, Emmanuel M.

doi:10.1590/s1516-8913201401485

Cited by 8 publications

(3 citation statements)

References 11 publications

(12 reference statements)

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“…In previous studies, other researchers have tried amylase optimization using the RSM method. Roy and Mukherjee, 37 Keharom et al 38 and Stergiou et al 39 found positive effects of starch, Zambare 40 and Mustafa et al 41 found positive effects of temperature, and Gangadharan et al 42 found positive effects of CaCl 2 on amylase production using RSM method. The variables of probability p-value <0.05 were considered significant, and p-value higher than 0.05 were not considered significant.…”

Section: One Variable At a Time Approach And Plackett-burman Designmentioning

confidence: 99%

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Bekler

Yalaz

Güven

et al. 2019

JSCS

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A novel amylolytic enzyme producing thermoalkaliphilic bacterium, the source of industrially used enzymes was isolated. Isolated strain was identified by morphological, physio-biochemical tests and the 16S rRNA gene sequence analysis. The optimal conditions of enzyme activity were determined. For higher α-amylase production, the variables such as yeast extract, starch, CaCl 2 , (NH 4) 2 SO 4 , NaCl and MgSO 4 in the α-amylase production medium, the temperature and pH were screened by Plackett-Burman design and optimised using response surface methodology (RSM). The optimal conditions were found to be 0.15 g/L for starch, 0.15 mg/L for CaCl 2 and 60 °C for temperature. By using RSM model, amylase production increase was achieved sevenfold. It is showed that this method can be utilised to optimize α-amylase production in athermophilic bacteria such as Bacillus paralicheniformis.

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Section: One Variable At a Time Approach And Plackett-burman Designmentioning

confidence: 99%

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Bekler

Yalaz

Güven

et al. 2019

JSCS

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“…Kluyveromyces marxianus IF0 0288 at pH 6.13 produces the highest quantity of alpha-amylase. 49 The optimized pH for the production of alpha-amylase by Penicillium notatum IBGE 03 has been reported to be 5.5. 42 Also, Bacillus sp.…”

Section: Alpha-amylase Productionmentioning

confidence: 99%

Microbial Alpha-Amylase Production: Progress, Challenges and Perspectives

et al. 2020

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Alpha-amylase reputes for starch modification by breaking of 1-4 glycosidic bands and is widely applied in different industrial sectors. Microorganisms express unique alpha-amylases with thermostable and halotolerant characteristics dependent on the microorganism’s intrinsic features. Likewise, genetic engineering methods are applied to produce enzymes with higher stability in contrast to wild types. As there are widespread application of α-amylase in industry, optimization methods like RSM are used to improve the production of the enzyme ex vivo. This study aimed to review the latest researches on the production improvement and stability of α-amylase.

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“…Several reports exist of successful RSM application, with methodologies like the Box-Behnken design, Plackett-Burhman factorial design, and central composite design (CCD), for process parameter optimization and attainment of enhanced enzyme production ([ [34] , [39] , [40] , [41] ]; Paul et al., 2021). RSM has been applied successfully for enhanced alpha-amylase production [ [4] , [42] , [43] , [44] , [45] , [46] ].…”

Section: Introductionmentioning

confidence: 99%

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

Ugwuoji

Nwagu

Ezeogu

2023

Biotechnology Reports

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Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology

Abstract: (up to 13-fold) in the production of extracellular α-amylase. These results constituted first evidence that K. marxianus could be potentially used as an effective source of extracellular α-amylase.

Cited by 8 publications

References 11 publications

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Optimization of the thermostable alkaline and Ca-dependent α−amylase production from Bacillus paralicheniformis by statistical modeling

Microbial Alpha-Amylase Production: Progress, Challenges and Perspectives

Detergent-stable amylase production by Paenibacillus lactis strain OPSA3 isolated from soil; optimization by response surface methodology

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