Quality Control of Biotechnological Inputs DetectingMycoplasma

Netto, Cristiane; Soccol, Vanete Thomaz; Sepulveda, L.; Garcia, Gabriel Henrique Oliveira; Timenetsky, Jorge

doi:10.1590/s1516-8913201400130

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…The human non-small cell lung cell line, H1299, pCB6+ vector, WT p53 and p53 H175 pCB6+ plasmids were kindly provided by Professor Karen Vousden (The Beatson Institute for Cancer Research, UK). Mycoplasma contamination was absent in all cell lines (28). Recombinant pCB6+ based plasmids carrying full-the length WT TP53 K100 and del TP53 M213 were constructed with cDNA following the RT-PCR amplification of RNA from KKU-100 and KKU-M213 cells.…”

Section: Methodsmentioning

confidence: 99%

p53‑p72‑Δ225‑331‑V31I identified in a cholangiocarcinoma cell line promotes migration and invasiveness via the downregulation of claudin‑1 expression and the activation of Cdc42

Puetkasichonpasutha

Suthiphongchai

2020

Oncol Rep

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TP53 is the most common gene mutated in human cancers, including in cholangiocarcinoma (CCA). The gain-of-function properties of p53 variants are often involved in cancer progression. The present study demonstrated that a truncated del p53 variant, del p53 M213 , exhibited gain-of-function properties and was highly expressed in the invasive liver fluke Opisthorchis viverrini-associated CCA cell line, KKU-M213. The del p53 M213 variant lacked exons 7-9 and contained a V31I substitution (p53-p72-Δ225-331-V31I). Stably transfected p53-null human non-small cell lung H1299 cells exhibited a del p53 M213 localization in both the cell cytosol and nucleus. Del p53 M213 lacked anti-growth functions, and instead enhanced migration and invasiveness. In addition, this p53 variant downregulated claudin-1 expression and promoted Cdc42 activation, consistent with the roles of claudin-1 and Cdc42 in inhibiting cell-cell dissociation and promoting cell migration, respectively. On the whole, although del p53 M213 is an important driver of cancer cell migration and invasiveness, other properties related to its novel gain-of-function properties require further investigation in order to develop effective treatment strategies for cancers bearing this truncated TP53 allele.

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Section: Methodsmentioning

confidence: 99%

p53‑p72‑Δ225‑331‑V31I identified in a cholangiocarcinoma cell line promotes migration and invasiveness via the downregulation of claudin‑1 expression and the activation of Cdc42

Puetkasichonpasutha

Suthiphongchai

2020

Oncol Rep

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The vaginal microflora changes in various stages of the estrous cycle of healthy female dogs and the ones with genital tract infections

et al. 2021

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Background Inflammatory diseases of reproductive tract in bitches are a common problem in veterinary practice. The inflammation can lead to serious health problems. Research to determine the correlation between the health status of females, phase of the cycle, age and bacterial flora of the genital tract has been ongoing for years, but the results obtained by individual authors are often contradictory. Results A total of 39 dogs were included in this study. Ten were qualified to the 1st group with genital tract infections (8 in anestrus and 2 in proestrus) and 29 to the 2nd group without such infections (16 in anestrus, 9 in proestrus and 4 in diestrus). The most common bacterial isolates obtained from the vaginal tract of all dogs were Escherichia coli, Staphylococcus pseudintermedius and Streptococcus canis. The prevalence of Gram-negative rods (other than E. coli) was significantly higher in the group with genital tract infections versus healthy dogs. There was no presence of Chlamydiaceae, Chlamydia abortus and lactic acid-producing bacteria in tested swabs. Conclusions Our study identified the most common bacteria in the genital tract of bitches. The total number of bacteria was almost the same in the healthy and infected dogs, as well as between the cycle stages. In our opinion, bacterial culturing of vaginal swab specimens from bitches without signs of genital disease is of little value. Furthermore, it should always be preceded by clinical examination and cytological examination of the vaginal epithelium.

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Quality Control of Biotechnological Inputs DetectingMycoplasma

Cited by 2 publications

References 20 publications

p53‑p72‑Δ225‑331‑V31I identified in a cholangiocarcinoma cell line promotes migration and invasiveness via the downregulation of claudin‑1 expression and the activation of Cdc42

p53‑p72‑Δ225‑331‑V31I identified in a cholangiocarcinoma cell line promotes migration and invasiveness via the downregulation of claudin‑1 expression and the activation of Cdc42

The vaginal microflora changes in various stages of the estrous cycle of healthy female dogs and the ones with genital tract infections

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