Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol

Gomaa, Eman Zakaria

doi:10.1590/s1516-89132014000100020

Cited by 81 publications

(59 citation statements)

References 24 publications

(23 reference statements)

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“…For solvent-based extraction of PHA, the concentrated biomass is dried either by freeze-or spraydrying. PHA is extracted directly from ground biomass by dissolving it in an organic solvent, usually chloroform, chlorolimited methylene chloride, propylene carbonate and dichloroethane (70,151,152,153). After removing cellular components (residuals) by filtration and centrifugation, the PHA is precipitated in a non-PHA-dissolving solvent such as cold ethanol or methanol (154)(155)(156)(157)(158).…”

Section: Methods For Extractionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

252

127

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Polyhydroxyalkanoates (PHAs) are intracellular aliphatic polyesters synthesized as energy reserves, in the form of water-insoluble, nano-sized discrete and optically dense granules in cytoplasm by a diverse bacteria and some archae under conditions of limiting nutrients in the presence of excess carbon source. Bacteria synthesize different PHAs from coenzyme A thioesters of respective hydroxyalkanoic acid, and degrade intracellularly for reuse and extracellularly in natural environments by other microorganisms. In vivo, PHAs exist as amorphous mobile liquid and water-insoluble inclusions but in vitro, exhibit material and mechanical properties, ranging from stiff and brittle crystalline to elastomeric and molding, similar to petrochemical thermoplastics. Further, they are hydrophobic, isotactic, biocompatible and exhibit piezoelectric properties. But as commodity plastics their applications are limited by high production cost, low yield, in vivo degradation, complexity of technology and difficulty of extraction. Therefore, to replace the conventional plastic with PHAs, it is prerequisite to standardize the PHA production systems.

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Section: Methods For Extractionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

252

127

View full text Add to dashboard Cite

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“…The spectra obtained for the samples presented identical functional bands. Methylene bonding was found apparent in patterns noted at wave number range 2923–2924 cm −1 . The band found at 1725 cm −1 is assigned to the axial deformation of the carbonyl group (CO).…”

Section: Resultsmentioning

confidence: 96%

Bioconversion of residual soybean oil into polyhydroxyalkanoates

Nascimento

Nunes

Rodrigues

et al. 2018

J of Applied Polymer Sci

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The aim of this study is to evaluate the bioconversion of residual soybean oil (RSO) into polyhydroxyalkanoates (PHAs) by selecting microorganism and fermentation condition in order to increase PHAs production. PHAs production by Cupriavidus necator IPT 026 using glucose (PHA 1) and RSO (PHA 2) as substrate is 1.15 ± 0.21 and 2.84 ± 0.04 g L−1, respectively. FTIR spectra of PHAs were similar to data reported in literature. PHAs presented low crystallinity (PHA 1: 42.69%; PHA 2: 46.44%), high thermal stability (PHA 1: 271.78 °C; PHA 2: 272.52 °C), and low MW (PHA 1: 140.69 kDa; PHA 2: 254.54 kDa). PHAs produced by RSO are potential candidates for industrial applications, especially ones that demand higher temperatures. This is the first study on the production and characterization of PHAs obtained by C. necator IPT 026 in culture with RSO. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46255.

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“…As it follows from a brief literature overview there are a lot of applications of metabolites (recombinant insulin [111], polyhydroxyalkanoates [112–113] etc . [114–119]) produced by the strains studied.…”

Section: Resultsmentioning

confidence: 99%

New tricks of well-known aminoazoles in isocyanide-based multicomponent reactions and antibacterial activity of the compounds synthesized

Murlykina

Kornet

Десенко

et al. 2017

Beilstein J. Org. Chem.

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The well-known aminoazoles, 3-amino-5-methylisoxazole and 5-amino-N-aryl-1H-pyrazole-4-carboxamides, were studied as an amine component in Ugi and Groebke–Blackburn–Bienaymé multicomponent reactions. The first example of an application of aminoazoles in an Ugi four-component reaction was discovered and novel features of a Groebke–Blackburn–Bienaymé cyclocondensation are established and discussed. The heterocycles obtained were evaluated for their antibacterial activity and several of them demonstrated a weak antimicrobial effect, but for most of the compounds a 30–50% increase in biomass of Gram-positive strains (mainly B. subtilis) compared to control was observed.

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Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol

Abstract: The aim of this work was to study the production of polyhydroxyalkanoates (PHAs)

Cited by 81 publications

References 24 publications

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Bioconversion of residual soybean oil into polyhydroxyalkanoates

New tricks of well-known aminoazoles in isocyanide-based multicomponent reactions and antibacterial activity of the compounds synthesized

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