Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)

Jiang, Yan‐Yi; Hu, Yanlin; Wang, Biao; Wu, Tianlong

doi:10.1590/s1516-89132013005000018

Cited by 15 publications

(8 citation statements)

References 27 publications

(20 reference statements)

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“…In another study, overexpression of CHS6 and IFS2 genes resulted in the reduced level of isoflavones and increased level of phenolic compounds ( Lozovaya et al, 2007 ). Jiang et al (2010 ; 2014 ) explored the negative regulation of F3H and GmFNSII genes by the RNAi-mediated gene silencing studies. Previously, the gene silencing of GmFNSII resulted in the increased accumulation of genistein in soybean hairy roots ( Jiang et al, 2010 ).…”

Section: Regulation Of Isoflavone Production Through Genetic Engineering In Soybeanmentioning

confidence: 99%

Metabolic Engineering of Isoflavones: An Updated Overview

Sohn

Subramanian

et al. 2021

Front. Plant Sci.

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Isoflavones are ecophysiologically active secondary metabolites derived from the phenylpropanoid pathway. They were mostly found in leguminous plants, especially in the pea family. Isoflavones play a key role in plant–environment interactions and act as phytoalexins also having an array of health benefits to the humans. According to epidemiological studies, a high intake of isoflavones-rich diets linked to a lower risk of hormone-related cancers, osteoporosis, menopausal symptoms, and cardiovascular diseases. These characteristics lead to the significant advancement in the studies on genetic and metabolic engineering of isoflavones in plants. As a result, a number of structural and regulatory genes involved in isoflavone biosynthesis in plants have been identified and characterized. Subsequently, they were engineered in various crop plants for the increased production of isoflavones. Furthermore, with the advent of high-throughput technologies, the regulation of isoflavone biosynthesis gains attention to increase or decrease the level of isoflavones in the crop plants. In the review, we begin with the role of isoflavones in plants, environment, and its benefits in human health. Besides, the main theme is to discuss the updated research progress in metabolic engineering of isoflavones in other plants species and regulation of production of isoflavones in soybeans.

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Section: Regulation Of Isoflavone Production Through Genetic Engineering In Soybeanmentioning

confidence: 99%

Metabolic Engineering of Isoflavones: An Updated Overview

Sohn

Subramanian

et al. 2021

Front. Plant Sci.

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“…These metabolites are known to exhibit anticancerous and anti-osteoporotic activities in animal systems. Jiang et al (2014) enhanced isoflavone production in soybean hairy roots by down-regulating two flavonoid genes, the F3H and the flavones synthase II ′′ . In tobacco, Pandey et al (2014) showed a substantial amount of genistein glycoconjugates as a result of the co-expression of the transcription factor AtMYB12 with the soybean IFS gene”.…”

Section: Engineering Of Flavonoid Pathwaymentioning

confidence: 99%

Flavonoids: a metabolic network mediating plants adaptation to their real estate

2014

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From an evolutionary perspective, the emergence of the sophisticated chemical scaffolds of flavonoid molecules represents a key step in the colonization of Earth’s terrestrial environment by vascular plants nearly 500 million years ago. The subsequent evolution of flavonoids through recruitment and modification of ancestors involved in primary metabolism has allowed vascular plants to cope with pathogen invasion and damaging UV light. The functional properties of flavonoids as a unique combination of different classes of compounds vary significantly depending on the demands of their local real estate. Apart from geographical location, the composition of flavonoids is largely dependent on the plant species, their developmental stage, tissue type, subcellular localization, and key ecological influences of both biotic and abiotic origin. Molecular and metabolic cross-talk between flavonoid and other pathways as a result of the re-direction of intermediate molecules have been well investigated. This metabolic plasticity is a key factor in plant adaptive strength and is of paramount importance for early land plants adaptation to their local ecosystems. In human and animal health the biological and pharmacological activities of flavonoids have been investigated in great depth and have shown a wide range of anti-inflammatory, anti-oxidant, anti-microbial, and anti-cancer properties. In this paper we review the application of advanced gene technologies for targeted reprogramming of the flavonoid pathway in plants to understand its molecular functions and explore opportunities for major improvements in forage plants enhancing animal health and production.

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“…The coexpression of Arabidopsis AtMYB12 and Lotus japonicus LjIFS in tomato f3h mutant also led to enhanced biosynthesis of isoflavones (Zhang et al , ). When GmF3H‐1 and GmFNSII‐2 were silenced separately or simultaneously in soya bean hairy roots by RNAi, the isoflavone content was strongly increased (Jiang et al , ; Jiang et al , ).…”

Section: Introductionmentioning

confidence: 99%

Multiplex CRISPR/Cas9‐mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus

Zhang

Wang

et al. 2019

Plant Biotechnology Journal

168

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and (Tel 86-25-84396410; fax 86-25-84396410; email dyyu@njau.edu.cn) † These authors contribute equally to this work. SummaryIsoflavonoids, which include a variety of secondary metabolites, are derived from the phenylpropanoid pathway and are distributed predominantly in leguminous plants. These compounds play a critical role in plant-environment interactions and are beneficial to human health. Isoflavone synthase (IFS) is a key enzyme in isoflavonoid synthesis and shares a common substrate with flavanone-3-hydroxylase (F3H) and flavone synthase II (FNS II). In this study, CRISPR/Cas9-mediated multiplex gene-editing technology was employed to simultaneously target GmF3H1, GmF3H2 and GmFNSII-1 in soya bean hairy roots and plants. Various mutation types and frequencies were observed in hairy roots. Higher mutation efficiencies were found in the T 0 transgenic plants, with a triple gene mutation efficiency of 44.44%, and these results of targeted mutagenesis were stably inherited in the progeny. Metabolomic analysis of T 0 triplemutants leaves revealed significant improvement in isoflavone content. Compared with the wild type, the T 3 generation homozygous triple mutants had approximately twice the leaf isoflavone content, and the soya bean mosaic virus (SMV) coat protein content was significantly reduced by one-third after infection with strain SC7, suggesting that increased isoflavone content enhanced the leaf resistance to SMV. The isoflavone content in the seeds of T 2 triple mutants was also significantly increased. This study provides not only materials for the improvement of soya bean isoflavone content and resistance to SMV but also a simple system to generate multiplex mutations in soya bean, which may be beneficial for further breeding and metabolic engineering. 1384

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Bivalent RNA interference to increase isoflavone biosynthesis in soybean (Glycine max)

Cited by 15 publications

References 27 publications

Metabolic Engineering of Isoflavones: An Updated Overview

Metabolic Engineering of Isoflavones: An Updated Overview

Flavonoids: a metabolic network mediating plants adaptation to their real estate

Multiplex CRISPR/Cas9‐mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus

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