Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate

Shalin, Thomas; Sindhu, Raveendran; Soccol, Carlos Ricardo; Pandey, Ashok

doi:10.1590/s1516-89132013005000016

Cited by 14 publications

(8 citation statements)

References 20 publications

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“…Mechanical tests were conducted using a Universal Testing Machine to investigate the mechanical properties of the XG matrix after the addition of PHB and plasticizers. The tensile strength of XG films can reach above 50 MPa at 0% relative humidity [19]. The films were stored at room temperature and exposed to the relative humidity of the surrounding.…”

Section: Mechanical Properties Of Phb Xg and Phb-xg Blendmentioning

confidence: 99%

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Production and Characterization of Polymer Blends and Assessment of Biodegradation

Sakthiselvan

Madhumathi

Karthick

et al. 2022

Journal of Nanomaterials

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Polyhydroxybutyrate (PHB), a microbial polyester well known for its high glass transition and melting temperatures, is found in the intracellular granule within microbes. Herein, we have attempted to synthesize PHB along with xyloglucan (XG) as a copolymer to lower glass transition and melting temperature. Fourier transform infrared spectroscopic (FTIR) and Scanning Electron Microscopic (SEM) analyses were used to investigate the characteristics of blend films. We found that the addition of XG resulted in increased moisture absorption of the blend films. XG-PHB blend of ratio 80 : 20 showed the highest tensile strength and was subjected to thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis. The results revealed that the XG-PHB blend has a lower melting point (121.3°C) and lesser crystalline degree (17.26%) than PHB. TGA results showed that blend film was more thermally stable than PHB. An effort to understand and analyze microorganisms that can produce and biodegrade copolymers such as PHB-XG is the main effort of this study which unfolds “new hope” in the field of biodegradable alternatives to plastics.

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Section: Mechanical Properties Of Phb Xg and Phb-xg Blendmentioning

confidence: 99%

“…of polyester blends containing PHB[19]. Studies also suggest that PHB degrades naturally and completely to carbon dioxide and water in the natural environment by the enzymatic action of microbes.…”

mentioning

confidence: 99%

Production and Characterization of Polymer Blends and Assessment of Biodegradation

Sakthiselvan

Madhumathi

Karthick

et al. 2022

Journal of Nanomaterials

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“…Mixed culture systems were shown to produce large amounts of PHAs in a wide range of low-cost substrates (Shalin et al, 2014). We have grown the best PHA-producers on three sugar substrates with a total concentration of 2% w/v.…”

Section: Effect Of Bacterial Combination and Sugar Mixtures On The Pha Productionmentioning

confidence: 99%

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Gnaim

Polikovsky

Unis

et al. 2021

Bioresource Technology

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The biosynthesis of polyhydroxyalkanoate (PHA) biopolymers from certain marine microbes, associated with green macroalgae Ulva sp., has attracted significant attention. The Ulva sp. is abundant biomass in numerous locations around the world and could be easily cultivated by marine farming. The variety of sugars found in Ulva sp. homogenate could be used as a carbon source for microbial growth and PHA production. In this work, we isolated and explored a series of bacterial strains that function as potential producers of P(3HB), utilizing a range of common sugars found in Ulva sp. Analysis of 16S rDNA gene-sequence revealed that the PHA-producing bacteria were phylogenetically related to species of the genus Cobetia, Bacillus, Pseudoaltermonas, and Sulfitobacter. The highest-yield of P(3HB) was observed in the case of new Cobetia strain, C. amphilecti, with up to 61% (w/w) in the presence of mannitol and 12% (w/w) on Ulva sp. acid hydrolysate as a substrate.

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“…In addition, by employing an artificial consortium of selected microorganisms, precision design of a defined microbial co- or multi-culture provides a comprehensive understanding of organismal interactions and allows examining the molecular and eco-physiological basis of community-level functions 19 , 20 . The developments in the field of artificial microbial ecosystem engineering allowed advancing in the aspects of ecology, such as soil bioremediation 21 and biotechnology, e.g., fine chemical 22 , 23 , biopolymer 24 , enzyme 25 , food additive 26 , antimicrobial 27 , biofuel 28 and biohydrogen production 29 – 31 . However, to achieve supreme efficiency of the bioprocess, a precision design strategy to form an artificial consortium of selected microorganisms was not yet considered.…”

Section: Introductionmentioning

confidence: 99%

Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia

et al. 2020

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Dark fermentative biohydrogen (H 2) production could become a key technology for providing renewable energy. Until now, the H 2 yield is restricted to 4 moles of H 2 per mole of glucose, referred to as the "Thauer limit". Here we show, that precision design of artificial microbial consortia increased the H 2 yield to 5.6 mol mol −1 glucose, 40% higher than the Thauer limit. In addition, the volumetric H 2 production rates of our defined artificial consortia are superior compared to any mono-, co-or multi-culture system reported to date. We hope this study to be a major leap forward in the engineering of artificial microbial consortia through precision design and provide a breakthrough in energy science, biotechnology and ecology. Constructing artificial consortia with this drawing-board approach could in future increase volumetric production rates and yields of other bioprocesses. Our artificial consortia engineering blueprint might pave the way for the development of a H 2 production bioindustry.

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Mixed Cultures Fermentation for the Production of Poly-ß-hydroxybutyrate

Abstract: Poly-β-hydroxybutyrate (PHB) is a biodegradable intracellular microbial product produced by many bacteria and it

Cited by 14 publications

References 20 publications

Production and Characterization of Polymer Blends and Assessment of Biodegradation

Production and Characterization of Polymer Blends and Assessment of Biodegradation

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Biohydrogen production beyond the Thauer limit by precision design of artificial microbial consortia

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