Cloning of Ammopiptanthus mongolicus C-repeat-binding factor gene and its cold-induced tolerance in transgenic tobacco

Abstract

C-repeat-binding factors (CBFs) are a type of important regulon in stress-

Overexpression of AmCBF1 enhances drought and cold stress tolerance, and improves photosynthesis in transgenic cotton

“…Increasing root to shoot ratio and decreasing plant stature are the main long-term plant adaptations to cold climates ( Körner, 2003 ). Additionally, electrolyte leakage, malondialdehyde, free proline, and soluble sugar are all important indicators of protection against drought or cold stress and have been widely substantiated ( Yue et al, 2012 ; Gu et al, 2013 ; Zhang et al, 2014 ). Drought and cold stress can damage the integrity of cellular membranes, causing electrolyte leakage and accumulation of macromolecules.…”

“…EU840990.1 ) gene was amplified and integrated into the pBI121 vector between the Xba I and BamH I restriction sites. The recombinant plasmid, pBI121- AmCBF1 , was introduced into the Agrobacterium tumefaciens strain LBA4404 ( Gu et al, 2013 ) to transform upland cotton variety R15 using the previously described protocol ( Wang et al, 2017 ). After germination, the regenerated plants with true leaves and roots were transferred to soil.…”

“…For drought treatment, the plants were irrigated twice weekly with 300 mM mannitol for 30 days. For the cold stress, the plants were treated at 4 °C for 48 h. Following treatment, the physiological traits of the seedlings were measured as previously described: relative water content (RWC) of the leaves ( Yue et al, 2012 ), electrolyte leakage from membranes in leaves ( Gu et al, 2013 ), chlorophyll content ( Arnon, 1949 ), and soluble sugar ( Lv et al, 2007 ). Each cotton line under stress treatment included at least three replicates (pots).…”

“…Overexpression of AmCBF1 , cloned from Ammopiptanthus mongolicus , an evergreen, broad-leaved shrub from the northwestern desert of China with great resistance to cold and aridity, significantly enhanced cold tolerance, reduced electrolytic leakage, and increased soluble sugar and proline content in transgenic tobacco compared to the wild type ( Gu et al, 2013 ). However, the application of AmCBF1 in regulating cotton resistance to drought and cold stress has not been extensively studied.…”