Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Bresolin, Iara Rocha Antunes Pereira; Bresolin, Igor Tadeu Lazzarotto; Silveira, Edgar; Tambourgi, Elias Basile; Mazzola, Priscila Gava

doi:10.1590/s1516-89132013000600012

Cited by 44 publications

(47 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ammonium sulfate saturation‐level precipitation of 20%–40% and 40%–60% gave higher specific protease activity and were used for the further purification, thus the complete protease recovery was unknown (Table ). The final purification fold of bromelain compared to it in the crude juice was up to 95.2 (Table ), which was 1–1.5 times higher than results reported in other research (Bresolin et al, ; Ketnawa et al, ; Li et al, ).…”

Section: Discussionmentioning

confidence: 52%

Fractionation and activity profiling of fruit bromelain from pineapples of Phuket variety growing in Thailand

et al. 2019

View full text Add to dashboard Cite

Bromelain is a type of protease found in both fruits and stems of pineapples. Stem bromelain has been extensively studied and is commercially available for applications in various industries. In contrast, studies of fruit bromelain are quite limited since most of pineapples have been consumed freshly, canned or juiced. Nowadays, the consumption of canned fruits, including canned pineapples has decreased greatly. Fruit bromelain could be a new growth point for pineapple industry. In this study, fruit bromelain was extracted from the pineapple juice of Phuket variety and some of its properties were studied. The enzyme was purified by precipitation using ammonium sulfate fractionation followed by ion‐exchange and gel filtration chromatography. Consequently, the protease purification level was increased by 95.2 fold. The final specific activity was getting to 448,590 U/mg on average, dominated by cysteine proteases, with optimal activity at 45°C and pH ranging from 6 to 8. The study facilitates the molecular and application research of fruit bromelain. Practical applications The research has been carried out at Funong Food Technology Co., Ltd., Guangdong, China, which produces primarily pineapple chunks and juice. As plenty of by‐products, like peels and cores of pineapples, are produced, the techniques are employed to extract bromelain from the by‐products. The techniques reported in this work are not new or advanced, however, they are applicable during the manufacturing process and the related equipment is easy to set up and operate. What's more, the practical application of the techniques is cost‐effective for the manufactories. Take Funong Food Technology Co., Ltd. as an example, they was using 80% saturation ammonium sulfate to precipitate protein from pineapple juice and obtained a bromelain with activity of approximately 8,000 U/mg and yield of 1.7 kg per ton of juice. With the application of the techniques reported in this work, bromelain was first extracted by ammonium sulfate gradient precipitation, and then purified through ion‐exchange and gel filtration chromatography. Each step of precipitation and purification generates a different level of activity and recovery of bromelain, ranging from around 2,506 to 448,590 U/mg, which allows for the production of bromelain according to the requirement of the market and brings more profits.

show abstract

Section: Discussionmentioning

confidence: 52%

Fractionation and activity profiling of fruit bromelain from pineapples of Phuket variety growing in Thailand

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The variation in the molecular weight of the enzyme might be due to differences in the bromelain source e.g. peel, fruit, stem and core; and other parameters such as pineapple species, age, harvesting and size (Bresolin et al 2013;Chaurasiya and Hebbar 2013).…”

Section: Physicochemical Properties Of Crude Pineapple Extractsmentioning

confidence: 99%

Characteristic properties of crude pineapple waste extract for bromelain purification by membrane processing

et al. 2015

View full text Add to dashboard Cite

Bromelain enzyme can be extracted from pineapple fruit including its waste parts. It is a valuable ingredient for various markets especially in the food and pharmaceutical areas. This enzyme can be extracted by membrane-based technology, but there is a lack of understanding of the properties of the crude pineapple extracts to link to functional membrane properties for efficient purification operation. This study focused on establishing characteristic properties of the crude pineapple waste mixture (CWM) extract, constituting of 57 % peel, 28 % crown and 15 % core, as a source of the enzyme and relevant to membr'ane processing. Rheological properties at different temperatures and pH levels were also determined in order to propose appropriate processing conditions for a practical and an efficient membrane operation. The CWM extract contained appreciable specific enzyme activity of 394.9 CDU/mg protein that would need to be purified at least 2-4 fold to achieve a required market standard. Existence of polysaccharides, particularly pectin, in the extract would be expected to cause fouling issues in membrane process, thus pre-treatment may be required to remove this compound from the mixture. The protein molecular weight of the CWM extract ranged between 11.7 and 26.9 kDa. The selection of membrane molecular weight cut-off (MWCO) should be above or below this range to isolate the enzyme. The evaluation on rheological properties of the CWM extract showed that it demonstrated lower viscosity at higher temperature and at neutral pH. Therefore, selection on temperature of 20-25°C and pH 7 throughout the membrane purification operation has been recommended.

show abstract

“…One promising protein that may be separated and purified by membranes is bromelain from pineapple extract (7). Bromelain is a generic name given to proteolytic enzymes found in vegetable tissues of pineapple (Ananas comosus) and another Bromeliaceae family (8). The enzyme is found in the stem (EC 3.4.22.32), fruit (EC 3.4.22.33) as well as in other parts of pineapple (9).…”

Section: Introductionmentioning

confidence: 99%

Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration

Nor

Ramchandran

Duke

et al. 2017

Food Technol. Biotechnol.

View full text Add to dashboard Cite

SummaryMembrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pre-treatment and diafiltration operation were employed in a two-stage ultrafiltration (UF) system to enhance the performance of the purification and concentration process of bromelain enzyme from an extract of pineapple crude waste mixture (CWM). Pre-treatment of the CWM extract using either pectinase or cellulase, or the combination of both, was applied and compared regarding the apparent viscosity reduction. Diafiltration step was introduced in UF stage 2 and observation on the flux performance, enzyme recovery and enzyme purity were performed. A 12 % apparent ftb.com.hr Please note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing.Food Technology and Biotechnology 56 (2) 2018 www.2 viscosity reduction has been achieved when the CWM extract was pre-treated with pectinase which led to 37-38 % improvement in the flux performance of both UF stages, as well as higher enzyme recovery in UF stage 1. The introduction of diafiltration mode in UF stage 2 has managed to sustain high flux values while yielded 4.4-fold enzyme purity (higher than a 2.5-fold purity achieved in our previous work); however, high diluent consumption was needed. The outcomes of this study showed that the flux performance and bromelain separation can be enhanced by reducing the viscosity with the employment of enzymatic pre-treatment and diafiltration operation. Thus, both techniques can be potentially applied in a large-scale membrane-based process for bromelain production.

show abstract

Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

Abstract: ABSTRACT

Cited by 44 publications

References 35 publications

Fractionation and activity profiling of fruit bromelain from pineapples of Phuket variety growing in Thailand

Fractionation and activity profiling of fruit bromelain from pineapples of Phuket variety growing in Thailand

Characteristic properties of crude pineapple waste extract for bromelain purification by membrane processing

Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration

Contact Info

Product

Resources

About