Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Zhang, Qiaoyan; Qian, Jianhua; Ma, Lingzhi

doi:10.1590/s1516-89132013000400003

Cited by 2 publications

(2 citation statements)

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“…Considering the use of a lipid-rich waste as a substrate for the PHAs accumulation, it is necessary for the hydrolysis of triglycerides into free fatty acids before their subsequent transformation to PHAs [ 98 ]. The bacteria that were potentially able to accumulate PHAs with a lipolytic activity were Acidovorax [ 99 ], Acinetobacter [ 100 ], Azospirillum [ 101 ], Chryseobacterium [ 102 ], Comamonas [ 103 ], Corynebacterium [ 104 ], Devosia [ 105 ], Dokdonella [ 106 ], Dyella [ 107 ], Ferruginibacter [ 108 ], Flavobacterium [ 109 ], Gemmobacter [ 77 ], Gordonia [ 110 ], Leifsonia [ 111 ], Lysobacter [ 112 ], Niveispirillum [ 113 ], Novosphingobium [ 114 ], Pandoraea [ 115 ], Polynucleobacter [ 83 ], Pseudomonas [ 98 ], Qipengyuania [ 116 ], Rhizobium [ 117 ], Stenotrophomonas [ 118 ], Taibaiella [ 119 ], Thermomonas [ 120 ], and Tsukamurella [ 121 ].…”

Section: Resultsmentioning

confidence: 99%

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

et al. 2022

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The biosynthesis of polyhydroxyalkanoates (PHAs) from industrial wastes by mixed microbial cultures (MMCs) enriched in PHA-accumulating bacteria is a promising technology to replace petroleum-based plastics. However, the populations’ dynamics in the PHA-accumulating MMCs are not well known. Therefore, the main objective of this study was to address the shifts in the size and structure of the bacterial communities in two lab-scale sequencing batch reactors (SBRs) fed with fish-canning effluents and operated under non-saline (SBR-N, 0.5 g NaCl/L) or saline (SBR-S, 10 g NaCl/L) conditions, by using a combination of quantitative PCR and Illumina sequencing of bacterial 16S rRNA genes. A double growth limitation (DGL) strategy, in which nitrogen availability was limited and uncoupled to carbon addition, strongly modulated the relative abundances of the PHA-accumulating bacteria, leading to an increase in the accumulation of PHAs, independently of the saline conditions (average 9.04 wt% and 11.69 wt%, maximum yields 22.03 wt% and 26.33% SBR-N and SBR-S, respectively). On the other hand, no correlations were found among the PHAs accumulation yields and the absolute abundances of total Bacteria, which decreased through time in the SBR-N and did not present statistical differences in the SBR-S. Acinetobacter, Calothrix, Dyella, Flavobacterium, Novosphingobium, Qipengyuania, and Tsukamurella were key PHA-accumulating genera in both SBRs under the DGL strategy, which was revealed as a successful tool to obtain a PHA-enriched MMC using fish-canning effluents.

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Section: Resultsmentioning

confidence: 99%

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

et al. 2022

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“…Recently, the inactivation of bacteria, viruses and fungi has been reported using supercritical carbon dioxide (SC-CO 2 ) technology [24][25][26]. The food and pharmaceutical industries are also using SC-CO 2 as a non-thermal sterilization technology at moderate to high pressure to preserve products from microbial effects [27,28]. Using carbon dioxide in its supercritical fluid form has been found to be an option in inactivating the microbes in the sterilization of food without any effect on the nutritional content [29].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid CO₂ Technique for Destruction of Pathogenic Fungal Spores in Solid Clinical Wastes

Noman

Rahman

Shahadat

et al. 2016

CLEAN Soil Air Water

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The present study deals with the inactivation of fungal spores in clinical waste using the advanced supercritical carbon-dioxide (SC-CO 2 ) treatment technique. The process of inactivation was investigated under different conditions of pressure, temperature, and processing time. The efficiency of SC-CO 2 was determined in terms of log reduction and inactivation rate of fungal spores. Almost 100% inactivation of initial fungal spores was obtained. Predicted and experimental log reduction of fungal spores was found to be 5.93 and 6.00, respectively, under optimal conditions (35 MPa, 75°C in 90 min). SC-CO 2 treated Aspergillus niger and Penicillium simplicissimum spores were examined using scanning electron microscopy which showed spore damage in the form of rupture, tear, and shape distortion. These damages confirmed complete spore inactivation when no renewed fungal growth occurred after the treated samples were cultured onto fresh media. The inactivation of fungal spores using SC-CO 2 and autoclave inhibits the production of lipase, cellulase, amylase, and protease enzymes. Thus, without addition of any chemicals, the SC-CO 2 sterilization method can be potentially used for the inactivation of microorganisms in clinical waste as well as in food and pharmaceutical products.

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Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Abstract: ABSTRACT

Cited by 2 publications

References 20 publications

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Supercritical Fluid CO₂ Technique for Destruction of Pathogenic Fungal Spores in Solid Clinical Wastes

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Mutation breeding of lipase-producing strain Flavobacterium sp. by supercritical CO2 with hydrazine hydrate

Abstract: ABSTRACT

Cited by 2 publications

References 20 publications

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Dynamics of PHA-Accumulating Bacterial Communities Fed with Lipid-Rich Liquid Effluents from Fish-Canning Industries

Supercritical Fluid CO2 Technique for Destruction of Pathogenic Fungal Spores in Solid Clinical Wastes

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Product

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Supercritical Fluid CO₂ Technique for Destruction of Pathogenic Fungal Spores in Solid Clinical Wastes