Germination, carbohydrate composition and vigor of cryopreserved Caesalpinia echinata seeds

Zanotti, Rafael Fonsêca; Motta, Ludymila Brandão; Bragatto, Juliano; Labate, Carlos Alberto; Salomão, Antonieta Nassif; Vendrame, Wagner A.; Cuzzuol, Geraldo Rogério Faustini

doi:10.1590/s1516-89132012000500004

Cited by 7 publications

(3 citation statements)

References 18 publications

(31 reference statements)

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“…Proteomic studies on the embryo of artificially aged wheat (Triticum aestivum L.) [4] and rice (Oryza sativa L.) [5] seeds showed that differentially expressed proteins (DEPs) were mainly involved in nutrient reservoir, enzyme activity and regulation, energy supply and metabolism, as well as defense and stress responses, which were consistent with the previous physiological and biochemical studies. Hitherto, multifarious practical strategies have been adopted to prevent or retard seed aging and vigor loss during storage, such as cryopreservation, ultra-dry storage, and free-oxygen environment [6][7][8]. However, for aged seeds, delayed germination and slow post-germination growth are still the two most restrictive problems impeding their application under field conditions [9].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds

Yan

Jia

Mao

2020

IJMS

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Although melatonin has been reported to play an important role in regulating metabolic events under adverse stresses, its underlying mechanisms on germination in aged seeds remain unclear. This study was conducted to investigate the effect of melatonin priming (MP) on embryos of aged oat seeds in relation to germination, ultrastructural changes, antioxidant responses, and protein profiles. Proteomic analysis revealed, in total, 402 differentially expressed proteins (DEPs) in normal, aged, and aged + MP embryos. The downregulated DEPs in aged embryos were enriched in sucrose metabolism, glycolysis, β-oxidation of lipid, and protein synthesis. MP (200 μM) turned four downregulated DEPs into upregulated DEPs, among which, especially 3-ketoacyl-CoA thiolase-like protein (KATLP) involved in the β-oxidation pathway played a key role in maintaining TCA cycle stability and providing more energy for protein translation. Furthermore, it was found that MP enhanced antioxidant capacity in the ascorbate-glutathione (AsA-GSH) system, declined reactive oxygen species (ROS), and improved cell ultrastructure. These results indicated that the impaired germination and seedling growth of aged seeds could be rescued to a certain level by melatonin, predominantly depending on β-oxidation, protein translation, and antioxidant protection of AsA-GSH. This work reveals new insights into melatonin-mediated mechanisms from protein profiles that occur in embryos of oat seeds processed by both aging and priming.

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Section: Introductionmentioning

confidence: 99%

Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds

Yan

Jia

Mao

2020

IJMS

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“…According to Zanotti et al (2012), no significant difference was found between the leaf fresh weight and number of leaf in Caesalpinia echinata.…”

Section: Discussionmentioning

confidence: 92%

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

2014

Int. J. Biosci.

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Satureja rechingeri, belonging to the Lamiaceae family, is one of the native range species of Iran. Considering the loss of Satureja rechingeri germplasm, due to the limited distribution, biotic and abiotic stresses, overgrazing of livestock, and generally the risks threatening the habitat of this species, fundamental actions need to be performed towards the preservation of Satureja rechingeri genetic diversity. Cryopreservation has the ability to preserve the seeds for a long time with much lower costs than other methods and without loss of seed viability. In the current study, treatments of vitrification, glycerol (30%), and the reduction of seed moisture before entering the liquid nitrogen were used. After one week, the treated seeds were removed from liquid nitrogen and were melted in sterile distilled water bath with a temperature of 42 °C. Then, the seeds were washed by sucrose solution (1.2 M) using shaker in three stages and were cultured with control seeds on sterile wet filter papers and transferred to a growth chamber. According to the obtained results of seed cryopreservation of Satureja rechingeri, no significant difference was found for all germination and seed growth characteristics except germination percentage and rate. Our results clearly indicate the effectiveness of this method for the seed preservation of Satureja rechingeri as a unique and endangered species for a very long time.

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“…One week after the start of the experiment, a fungus infection was detected in about 5% of the immersion groups, apparently of endogenous origin. These were discarded and contents of each bottle were treated with 50 mg of nystatin (0.1% concentration) (Zanotti et al 2012, Luz 2008 to prevent further contamination.…”

Section: Methodsmentioning

confidence: 99%

Seawater resistance in sweet potato (Ipomoea batatas) seeds: a key factor for natural dispersal from the Americas to Oceania

Pereira¹,

Nunes²,

Pessenda³

et al. 2020

Frontiers of Biogeography

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Highlights • Several hypotheses try to explain the presence of pre-historical records of sweet potato in both the American continent and French Polynesia, ranging from hydrochory, zoochory, and anthropochory, the latter being a potential evidence of pre-Columbian contacts between native Americans and Polynesians. • We tested the viability of sweet potato seeds following seawater immersion as a basic requirement for any biogeographical hypotheses involving sea-drift and seeds. • All seeds within our trial germinated regardless of seawater immersion periods, corroborating modeling studies that estimate a 120-day seed dispersal period from the Americas to French Polynesia. • The sweet potato's resistance to seawater is a novel feature we described for this species and substantially weakens a potential hindrance to the sea-drift natural dispersal hypothesis, being an important addition to biogeographical studies for this species. Plant science research can also benefit from our results due to potential agronomical advantages of salt-tolerant sweet potato seeds. Our results are neutral with regards to the hypothesis that presupposes a relationship between historical sweet potato presence in both continents and pre-Columbian human contacts. This could have occurred either with or without seed resistance to seawater.

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Germination, carbohydrate composition and vigor of cryopreserved Caesalpinia echinata seeds

Abstract: The present study investigated the germination and vigor of

Cited by 7 publications

References 18 publications

Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds

Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

Seawater resistance in sweet potato (Ipomoea batatas) seeds: a key factor for natural dispersal from the Americas to Oceania

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