Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces

Zeraik, Ana Eliza; Nitschke, Márcia

doi:10.1590/s1516-89132012000400012

Cited by 50 publications

(35 citation statements)

References 24 publications

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“…Under laboratorial conditions, Kadam et al 13 observed that nutrient availability affected biofilm formation by L. monocytogenes strains in polystyrene surfaces, highlighting that nutritionally poor media favored the growth in biofilm state. On the other hand, Zeraik and Nitschke 14 demonstrated that L. monocytogenes produced more biofilm in nutrient rich medium. In the present study, BHI broth was used because it has been reported as an effective medium to promote biofilm formation by L. monocytogenes isolates from different origins, at various surfaces 2, 15.…”

Section: Discussionmentioning

confidence: 99%

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Reis‐Teixeira

Alves

Martinis

2017

Brazilian Journal of Microbiology

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The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were examined using regular fluorescence microscopy, confocal laser scanning microscopy and classic culture method. The biofilms formed were not very dense and microscopic observations revealed uneven biofilm structures, with presence of exopolymeric matrix surrounding single cells, small aggregates and microcolonies, in a honeycomb-like arrangement. Moreover, planktonic population of L. monocytogenes (present in broth media covering the abiotic surface) remained stable throughout the incubation time, which indicates an efficient dispersal mechanism, since the culture medium was replaced daily. In conclusion, even if these strains of L. monocytogenes were not able to form thick multilayer biofilms, it was noticeable their high persistence on abiotic surfaces, reinforcing the need to focus on measures to avoid biofilm formation, instead of trying to eradicate mature biofilms.

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Section: Discussionmentioning

confidence: 99%

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Reis‐Teixeira

Alves

Martinis

2017

Brazilian Journal of Microbiology

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“…There was an increase (up to 50%; Table 2) in count for both groups of organisms, during the rainy season months of March to September but no significant difference (p > 0.05) was observed in the count of organisms between all months evaluated. It has been explained (Zeraik and Nitschke, 2012) that, environmental conditions can influence the complex process of bacterial adhesion to inert surfaces. Hence, the higher counts between March and September among the months analysed for microbial count (Table 2) may be due to, the prolonged period that the bottling equipment was kept idle which allowed microorganisms to proliferate.…”

Section: Resultsmentioning

confidence: 99%

Prevalence and risk of heterotrophic microorganisms in a carbonated soft drink factory

Nwaiwu¹,

Ibekwe²

2017

Afr. J. Microbiol. Res.

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A farm to fork approach was used to establish the prevalence and risk of microorganisms in a carbonated soft drinks factory in south eastern Nigeria. Raw materials, intermediate and finished products were collected from 19 microbiological control points in a processing environment and analysed using membrane filtration over a 12 month period. Yeasts and other heterotrophic bacteria increased during the rainy season but there was no significant difference (p>0.05) in the proliferation of organisms among the analysed months. Although, organisms were not detected in finished products, there was 100% prevalence for yeasts and bacteria in the processing areas with high sugar activity. No mould, Pseudomonas aeruginosa or Escherichia coli were isolated. Overall, a 5 by 5 risk matrix showed that heterotrophic bacteria, yeasts or mould had low risk of reaching undesirable numbers. In addition to the prevalence investigation carried out in the processing environment, 864 properly stored bottles of the same brand of carbonated soft drink in trade were purchased from different commercial locations over 12 months and screened. No yeasts mould or coliform bacteria were isolated from the sampling carried out. In conclusion, heterotrophic bacteria and yeasts thrive more on equipment than intermediate or finished products in the process environment studied and the risks of product spoilage or people getting ill was generally low and may remain so, if good manufacturing and proper after sales handling of the products are observed. Study highlights the need to focus on areas of high sugar activity, to control undesirable organisms and further work is required to establish how microorganisms survive and form biofilms on bottling equipment after sanitation.

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“…The key non-enzymatic antioxidant -glutathione, both in reduced and oxidized forms, was found at highest level in the biofilm cells. The formation of bacterial biofilm on the surfaces of materials has been considered, with a few exceptions (Jayaraman et al 1997), as a highly undesirable processes (Geesey 2001;O'Toole 2003;MacKintosh et al 2006;Subramani et al 2009;Zeraik and Nitschke 2012). It is particularly undesirable for biomaterial surfaces exposed to a constant contact with the tissues and body fluids of living organism.…”

Section: Resultsmentioning

confidence: 99%

Resistance of oxidative stress in biofilm and planktonic cells

Jakubowski

Walkowiak

2015

Braz. arch. biol. technol.

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Influence of growth media and temperature on bacterial adhesion to polystyrene surfaces

Cited by 50 publications

References 24 publications

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces

Prevalence and risk of heterotrophic microorganisms in a carbonated soft drink factory

Resistance of oxidative stress in biofilm and planktonic cells

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