In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Delmail, David; Labrousse, Pascal; Lesouëf, Jean-Yves; Hir, François Le; Autret, Jean-Luc; Botineau, Michel

doi:10.1590/s1516-89132012000400009

Cited by 3 publications

(2 citation statements)

References 18 publications

(15 reference statements)

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“…) and recovery attempts based on only a very small number of founders (e.g., Normania triphylla one individual) (Delmail et al. ). From an evolutionary point of view, material stored in germplasm banks and herbaria is only representative of the time at which it was collected, whereas environmental factors impose evolutionary changes in extant plant populations (Lowe et al.…”

Section: General Obstacles To the Recovery Of Plant Genetic Diversitymentioning

confidence: 99%

“…Unintentional selection after several generations in ex situ cultivation of short-lived species make propagules unsuitable for their reintroduction. The tendency to have low quantities of seed or plant stocks preserved ex situ is often reflected in reduced genetic diversity of ex situ collections (Sarasan et al 2016) and recovery attempts based on only a very small number of founders (e.g., Normania triphylla one individual) (Delmail et al 2012). From an evolutionary point of view, material stored in germplasm banks and herbaria is only representative of the time at which it was collected, whereas environmental factors impose evolutionary changes in extant plant populations (Lowe et al 2000).…”

Section: General Obstacles To the Recovery Of Plant Genetic Diversity...mentioning

confidence: 99%

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Ex situ collections and their potential for the restoration of extinct plants

Abeli

Dalrymple

Godefroid

et al. 2019

Conservation Biology

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The alarming current and predicted species extinction rates have galvanized conservationists in their efforts to avoid future biodiversity losses, but for species extinct in the wild, few options exist. We posed the questions, can these species be restored, and, if so, what role can ex situ plant collections (i.e., botanic gardens, germplasm banks, herbaria) play in the recovery of plant genetic diversity? We reviewed the relevant literature to assess the feasibility of recovering lost plant genetic diversity with using ex situ material and the probability of survival of subsequent translocations. Thirteen attempts to recover species extinct in the wild were found, most of which used material preserved in botanic gardens (12) and seed banks (2). One case of a locally extirpated population was recovered from herbarium material. Eight (60%) of these cases were successful or partially successful translocations of the focal species or population; the other 5 failed or it was too early to determine the outcome. Limiting factors of the use of ex situ source material for the restoration of plant genetic diversity in the wild include the scarcity of source material, low viability and reduced longevity of the material, low genetic variation, lack of evolution (especially for material stored in germplasm banks and herbaria), and socioeconomic factors. However, modern collecting practices present opportunities for plant conservation, such as improved collecting protocols and improved cultivation and storage conditions. Our findings suggest that all types of ex situ collections may contribute effectively to plant species conservation if their use is informed by a thorough understanding of the aforementioned problems. We conclude that the recovery of plant species currently classified as extinct in the wild is not 100% successful, and the possibility of successful reintroduction should not be used to justify insufficient in situ conservation.

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Section: General Obstacles To the Recovery Of Plant Genetic Diversitymentioning

confidence: 99%

Section: General Obstacles To the Recovery Of Plant Genetic Diversity...mentioning

confidence: 99%

Ex situ collections and their potential for the restoration of extinct plants

Abeli

Dalrymple

Godefroid

et al. 2019

Conservation Biology

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Micropropagation ofMyriophyllum Alterniflorum(Haloragaceae) for Stream Rehabilitation: FirstIn VitroCulture and Reintroduction Assays of a Heavy-Metal Hyperaccumulator Immersed Macrophyte

Delmail

Labrousse

Hourdin

et al. 2013

International Journal of Phytoremediation

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Nowadays, submersed aquatic macrophytes play a key role in stream ecology and they are often used as biomonitors of freshwater quality. So, these plants appear as natural candidates to stream rehabilitation experiments. Among them, the stream macrophyte Myriophyllum alterniflorum is used recently as biomonitor and is potentially useful for the restoration of heavy-metal contaminated localities. The best way to obtain a mass production of watermilfoil plants is micropropagation. We developed in vitro culture of M. alterniflorum and the effects of five media on the plant development were assessed. Five morphological and four physiological endpoints were examined leading to the recommendation of the Murashige and Skoog medium for ecotoxicological studies on chlorophyllous parts, and of the Gaudet medium for root cytotoxicity and phytoremediation studies. Micropropagated clones were acclimatized in a synthetic medium and in situ reintroduction was performed efficiently. This is the first report of micropropagated plants transplantation in streams. The successful establishment of watermilfoil beds even in polluted areas strongly suggested that ecological restoration using micropropagated watermilfoil is a promising biotechnology for phytoremediation and rehabilitation of degraded areas. Moreover, high bioconcentration factors evidenced that watermilfoil hyperaccumulates Cd and Cu, and could be potentially used in phytoremediation studies.

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Micropropagation d’Atropa belladonna et de Mandragora officinarum : évaluation du développement sur différents milieux de culture et analyse de quelques marqueurs biologiques

GALLOIS

Dulaurent

Morichon

et al. 2022

ASL

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La belladone et la mandragore ont été cultivées in vitro, sur le milieu de MS dans un premier temps avec douze équilibres de régulateurs hormonaux (6-benzyl aminopurine et acide 1-naphtalène acétique). Quelques marqueurs biologiques ont été dosés ; il s'agit des chlorophylles a, b et des caroténoïdes, des protéines totales, du potentiel osmotique et des alcaloïdes tropaniques (atropine et scopolamine). La deuxième phase de culture a consisté à cultiver la belladone sur les milieux 0/0 et 7,5/5 (BAP/ANA) et la mandragore sur le milieu de Murashige et Skoog (0,11 µM/0,89 µM). Lors de cette deuxième phase, les protéines, le potentiel osmotique et les alcaloïdes tropaniques ont été dosés dans la belladone. Dans la mandragore, seules l'atropine et la scopolamine ont été recherchées puisque, quel que soit l'équilibre en phytohormones, les cals ne se sont jamais différenciés. Les résultats obtenus, en CL-SM/SM, ont montré que chez la belladone, le milieu sans hormone était celui qui permettait d'obtenir les plus fortes concentrations en atropine et en scopolamine. Toutefois, ces valeurs restent bien inférieures à celles trouvées dans la littérature. La mandragore, quant à elle, n'a pratiquement pas produit de métabolites secondaires.

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In vitro establishment and multiplication of the Normania triphylla (Lowe) Lowe

Abstract: Normania triphylla is an endemic species from Madeira island (Portugal)

Cited by 3 publications

References 18 publications

Ex situ collections and their potential for the restoration of extinct plants

Ex situ collections and their potential for the restoration of extinct plants

Micropropagation ofMyriophyllum Alterniflorum(Haloragaceae) for Stream Rehabilitation: FirstIn VitroCulture and Reintroduction Assays of a Heavy-Metal Hyperaccumulator Immersed Macrophyte

Micropropagation d’Atropa belladonna et de Mandragora officinarum : évaluation du développement sur différents milieux de culture et analyse de quelques marqueurs biologiques

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