Assessment of the metabolism of different strains of Bacillus megaterium

Suarez, Carlos Alberto Galeano; Montano, Inti Doraci Cavalcanti; Nucci, Edson Romano; Iemma, Mônica Rosas da Costa; Giordano, Raquel de Lima Camargo; Giordano, Roberto de Campos

doi:10.1590/s1516-89132012000400001

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…In this experiment, B.megaterium released acetate using glucose and guar gum was hydrolysed [8]. Therefore, the complex lost viscosity and turned into a liquid form.…”

Section: The Difference Of Bmegaterium Growth On Dependence To Glucose Treatment On Guar Gum Hydrogelmentioning

confidence: 99%

Guar Gum Hydrogel Microbial Fuel Cell Using B.MEGATERIUM

Kim

2021

EJMSE

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The main factors of a microbial fuel cell (MFC) are the anode and the microorganisms around it that produce electrons. This research focuses on increasing the efficiency of a MFC in plant-MFC (PMFC) or aquarium-like conditions by inoculating microorganisms that help produce electrons to the soil. Guar gum was used as a base structure as it can culture microorganisms and create a hydrogel by cross-linking with borax. [1,2] Bacillus megaterium was added to the hydrogel as it can survive under the basic conditions of borax while producing electrons. [3] Glucose was added as well to help culture the bacteria. Then, the change of voltage was observed along with the growth of B.megaterium. A compound consisting of 0.5% glucose, 1% Guar gum, 10% Borax, and B.megaterium culture medium was created with a volume ratio of 1:25:1:1 in each order. This compound will be named GGB-B. GGB-B was able to keep its shape as a hydrogel along with a voltage increase of 250mV compared to control due to the stable growth of B.megaterium. 100g of GGB-B was placed inside a beaker and was covered with aquarium soil. Two goldfishes (Carassius auratus) were added. Voltage, pH, and the health of goldfishes were observed for 9 days. The result was approximately a 150mV increase in voltage compared to control (tap water). pH decrease was about 1.3, showing a pH of 6.12. There was no change in the goldfishes, and the GGB-B under the aquarium soil fused with the soil as it disintegrated. Therefore, when electron-producing microorganisms such as B.megaterium are added to guar gum, the microorganisms would slowly spread over the soil, thereby increasing the efficiency of MFC. This could also apply to PMFC and aquarium-like conditions as well.

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“…In this experiment, B.megaterium released acetate using glucose and guar gum was hydrolysed [8]. Therefore, the complex lost viscosity and turned into a liquid form.…”

Section: The Difference Of Bmegaterium Growth On Dependence To Glucose Treatment On Guar Gum Hydrogelmentioning

confidence: 99%

Guar Gum Hydrogel Microbial Fuel Cell Using B.MEGATERIUM

Kim

2021

EJMSE

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“…Using B. megaterium for this type of study is advantageous over the other non E. coli organisms, as it is a more stable system (Suarez et al 2012). It has been used as a new model to study the effect of pathway modifications on shikimic acid yield.…”

Section: Introductionmentioning

confidence: 99%

Studies on the production of shikimic acid using the aroK knockout strain of Bacillus megaterium

Ghosh¹,

Mohan

Banerjee³

2016

World J Microbiol Biotechnol

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Shikimic acid has various pharmaceutical and industrial applications. It is the sole chemical building block for the antiviral drug oseltamivir (Tamiflu(®)) and one of the potent pharmaceutical intermediates with three chiral centres. Here we report a modified strain of Bacillus megaterium with aroK (shikimate kinase) knock out to block the aromatic biosynthetic pathway downstream of shikimic acid. Homologous recombination based gene disruption approach was used for generating aroK knock out mutant of B. megaterium. Shake flask cultivation showed shikimic acid yield of 2.98 g/L which is ~6 times more than the wild type (0.53 g/L). Furthermore, the shikimate kinase activity was assayed and it was 32 % of the wild type. Effect of various carbon sources on the production of shikimic acid was studied and fructose (4 %, w/v) was found to yield maximum shikimic acid (4.94 g/L). The kinetics of growth and shikimic acid production by aroK knockout mutant was studied in 10 L bioreactor and the yield of shikimic acid had increased to 6 g/L which is ~12 fold higher over the wild type. It is evident from the results that aroK gene disruption had an immense effect in enhancing the shikimic acid production.

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“…nas amostras, mesmo após tratamento térmico, justificando os dados encontrados no Assentamento Independência.Quanto ao Bacillus megaterium, é uma bactéria geralmente proveniente do solo, mas podendo ser encontrada em alimentos desidratados, água marinha, sedimentos, peixes, flora normal e até mesmo mel de abelha. Essa é uma bactéria gram-positiva, aeróbica, formadora de endósporos, utilizada na secreção de proteínas recombinantes(SUAREZ et al, 2012). Provavelmente, esse micro-organismo veio a habitar a superfície do teto através do contato das cabras com o solo.…”

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Ciências agrárias: a multidisciplinaridade dos recursos naturais

2021

Ciências Agrárias: A Multidisciplinaridade Dos Recursos Naturais

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Resumo: O termo "línguas negras" designa, em linguagem popular, as águas residuais sem tratamento e qualquer tipo de esgoto que deságue em rios, mares, valas, canais, lagos e lagunas. Este trabalho teve como objeto de estudo a identificação e mapeamento das "línguas negras" existentes nas praias urbanas de Maceió utilizando técnicas de geoprocessamento. Na convergência dos fatores mencionados, o trabalho demanda diagnosticar, por meio de mapeamento e geoprocessamento, a situação ambiental das "línguas negras" existentes nas praias urbanas de Maceió. Diante da formação desse fato, passou-se a investigar os indicadores formadores e de contribuição, a saber: as galerias pluviais e as bacias hidrográficas formadoras dessas "línguas negras". A pesquisa foi realizada através de levantamentos feitos em bancos de dados de empresas e órgãos públicos, trabalho de campo, análises estatísticas e interpretações de documentos cartográficos digitais, tendo como produto, diferentes mapeamentos das variáveis espaciais definidoras do problema estudado. Com o auxílio de um GPS, as línguas negras foram georreferenciadas e plotadas na base cartográfica de Maceió, definindo assim, a área de estudo. O estudo demonstrou que a maioria das línguas negras encontrase exatamente no final das galerias pluviais, evidenciando assim, a possibilidade da existência de ligações clandestinas nesta rede de galerias.

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Assessment of the metabolism of different strains of Bacillus megaterium

Cited by 4 publications

References 11 publications

Guar Gum Hydrogel Microbial Fuel Cell Using B.MEGATERIUM

Guar Gum Hydrogel Microbial Fuel Cell Using B.MEGATERIUM

Studies on the production of shikimic acid using the aroK knockout strain of Bacillus megaterium

Ciências agrárias: a multidisciplinaridade dos recursos naturais

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