Viability in the production of a drug extracted from Ananas comosus by a flat membrane system

Lopes, Francisco Luiz Gumes; Sbruzzi, Dalva; Barros, Kleber Vânio Gomes; Ferreira, Juliana Ferrari; Santana, José Carlos Curvelo; Souza, Roberto Rodrigues de; Tambourgi, Elias Basile

doi:10.1590/s1516-89132012000300018

Cited by 8 publications

(6 citation statements)

References 19 publications

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“…The total fermentation process lasted 336 h. The wines were clarified using a 1% bentonit clay solution, were placed in 1.0 L ambercolored bottles, were chemically characterized according to the Brazilian laws, and were stored in a refrigerator at 5°C for a period of 6 months (Almeida et al, 2010;CurveloSantana et al, 2010a, b;Severo Jr. et al, 2007). The evaluation of the costs to obtain the product was made based on the methods introduced by Almeida et al (2013b), Castilho et al (2000), De Castro et al (2010), Giraçol et al (2011), Lopes et al (2012) and Passarini et al (2014).…”

Section: Minimum Price and Cost Determinationmentioning

confidence: 99%

“…The average monthly wage paid per employee (US$ 763.55) and the fee charged (38%) for these salary payments are based on the Brazilian micro-company laws (Almeida et al, 2013b;Lopes et al, 2012). The sale price of machines, tanks, and other machinery are available at Maquinas Sigma (2013) site; the other values were obtained from (Almeida et al, 2013b;Giraçol et al, 2011;Passarini et al, 2014).…”

Section: Sensory Analysismentioning

confidence: 99%

“…The total percentage including tax, shipping, commissions, and advertising was 38%, and a makeup range of 25% was used in calculation (Almeida et al, 2013b;De Castro et al, 2010;Lopes et al, 2012).…”

Section: Winecost Wagecost Fixedcost Minimum Price Productioncapacitymentioning

confidence: 99%

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Use of simulated annealing in standardization and optimization of the acerola wine production

Almeida¹,

Alves²,

Araújo³

et al. 2014

Food Sci. Technol (Campinas)

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Section: Minimum Price and Cost Determinationmentioning

confidence: 99%

Section: Sensory Analysismentioning

confidence: 99%

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Use of simulated annealing in standardization and optimization of the acerola wine production

Almeida¹,

Alves²,

Araújo³

et al. 2014

Food Sci. Technol (Campinas)

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“…The application of membrane-based processes for the separation and concentration of bromelain from extract of various pineapple parts involved a dual-stage microfiltration (MF) and ultrafiltration (UF) (11,12), which can be integrated with ammonium sulfate extraction and reverse micellar system (13,14). However, fine tuning the membrane separation to avoid the use of additional compounds like ammonium sulfate serves a green technology option offering great advantages for bromelain production.…”

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confidence: 99%

Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration

Nor

Ramchandran

Duke

et al. 2017

Food Technol. Biotechnol.

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SummaryMembrane technology has been successfully applied for the purification of bromelain, a protease enzyme from pineapple. However, the current system operates less optimally in terms of flux and separation primarily due to properties of the feed, such as viscosity. Hence, in this study, enzymatic pre-treatment and diafiltration operation were employed in a two-stage ultrafiltration (UF) system to enhance the performance of the purification and concentration process of bromelain enzyme from an extract of pineapple crude waste mixture (CWM). Pre-treatment of the CWM extract using either pectinase or cellulase, or the combination of both, was applied and compared regarding the apparent viscosity reduction. Diafiltration step was introduced in UF stage 2 and observation on the flux performance, enzyme recovery and enzyme purity were performed. A 12 % apparent ftb.com.hr Please note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing.Food Technology and Biotechnology 56 (2) 2018 www.2 viscosity reduction has been achieved when the CWM extract was pre-treated with pectinase which led to 37-38 % improvement in the flux performance of both UF stages, as well as higher enzyme recovery in UF stage 1. The introduction of diafiltration mode in UF stage 2 has managed to sustain high flux values while yielded 4.4-fold enzyme purity (higher than a 2.5-fold purity achieved in our previous work); however, high diluent consumption was needed. The outcomes of this study showed that the flux performance and bromelain separation can be enhanced by reducing the viscosity with the employment of enzymatic pre-treatment and diafiltration operation. Thus, both techniques can be potentially applied in a large-scale membrane-based process for bromelain production.

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“…The fruit is also a good option for its source as it has higher proteolytic activity than in the stem. A good quantity of fruit residue is generated by the pine apple processing factories, which can be used as raw material (Ferreira et al 2011;Fileti et al 2009 and2010;Lopes et al 2012;Parks 2001). In vitro cultivation is an important procedure in the multiplication of different species, This technique can be applied on the industrial scale.…”

Section: Introductionmentioning

confidence: 99%

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Leite

Lima

Santana

et al. 2012

Braz. arch. biol. technol.

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This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's

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Viability in the production of a drug extracted from Ananas comosus by a flat membrane system

Cited by 8 publications

References 19 publications

Use of simulated annealing in standardization and optimization of the acerola wine production

Use of simulated annealing in standardization and optimization of the acerola wine production

Performance of a Two-Stage Membrane System for Bromelain Separation from Pineapple Waste Mixture as Impacted by Enzymatic Pre-Treatment and Diafiltration

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

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