Immobilization of Bacillus megaterium MTCC 2444 by Ca-alginate entrapment method for enhanced alkaline protease production

Mrudula, S.; Shyam, Nidhi

doi:10.1590/s1516-89132012000100017

Cited by 29 publications

(15 citation statements)

References 22 publications

(34 reference statements)

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“…Higher inoculum concentrations may cause decrease of nutrients or autolysis of the enzymes and resulted in less growth and alkaline protease production. This result is in line with previous studies by Mrudula and Shyam 33 for yielding maximum protease production by immobilized B. megaterium MTCC 2444. However, optimum protease production with 10% of inoculum size was reported by Nadeem et al 34 .…”

Section: Determination Of Optimum Growth Conditionssupporting

confidence: 93%

“…These results comply with the literature reports where 40 C is the best temperature for alkaline protease production 33 and by newly isolated Bacillus spp 35 . In addition, Singh et al 36 reported that Bacillus sp.…”

Section: Determination Of Optimum Growth Conditionssupporting

confidence: 93%

“…Nearly 100% of enzyme activity was still detectable at pH 13.0 after 20 min at 30 C. This might be the alkaline protease. The protease activity had relative activity $80% in NaOHglycine-NaCl buffer pH ranging from 6 to 10.5 33 . This was in agreement with a research by Chomsri 43 who reported a protease activity which was stable within the pH range from 7 to 10.…”

Section: Temperature and Ph Stabilitymentioning

confidence: 99%

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Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification

Özçelik

Aytar

Gedikli

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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In this study, protease-producing capacity of Bacillus pumilus D3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. Optimum growing conditions for B. pumilus D3 in terms of protease production were determined as 1% optimum inoculum size, 35 C temperature, 11 pH and 48 h incubation time, respectively. Stability studies indicated that the mentioned protease was stable within the pH range of 7-10.5 and between 30 C and 40 C temperatures. Surprisingly, the activity of the enzyme increased in the presence of SDS with concentration up to 5 mM. The protease was concentrated 1.6-fold with ammonium sulfate precipitation and dialysis. At least six protein bands were obtained from dialysate by electrophoresis. Four clear protein bands with caseinolytic activity were detected by zymography. Dialysate was further purified by anion-exchange chromatography and the caseinolytic active fraction showed a single band between 29 and 36 kDa of reducing conditions.

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Section: Determination Of Optimum Growth Conditionssupporting

confidence: 93%

Section: Determination Of Optimum Growth Conditionssupporting

confidence: 93%

Section: Temperature and Ph Stabilitymentioning

confidence: 99%

See 1 more Smart Citation

Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification

Özçelik

Aytar

Gedikli

et al. 2013

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…when grown in a fermenter. Similarly, Mrudula and Shyam [70] reported a shorter incubation period of 48 h for maximum production of xylanase from B. megaterium MTTC 2444. In another study, some strains of Bacillus showed maximum xylanase production after 24 h using digested bran and 48 h of fermentation using sawdust as substrates, respectively [53].…”

Section: Discussionmentioning

confidence: 86%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 • C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 • C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications.

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“…and reported that maximum xylanase production was observed in 48 h and 72 h using wheat bran and corn cob as a substrate, respectively. Mrudula and Shyam [69] reported 48 h of fermentation time for maximum production of protease from B. megaterium MTTC 2444. Sepahy et al [45] reported a fermentation period of 48 h by Bacillus mojavensis AG137 in submerged fermentation using oat bran as a substrate.…”

Section: Resultsmentioning

confidence: 99%

Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass

Nkohla¹,

Okaiyeto²,

Nwodo³

et al. 2017

Pol. J. Environ. Stud.

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Immobilization of Bacillus megaterium MTCC 2444 by Ca-alginate entrapment method for enhanced alkaline protease production

Abstract: Optimization of culture conditions and immobilization parameters for alkaline protease production was

Cited by 29 publications

References 22 publications

Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification

Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Endoglucanase and Xylanase Production by Chryseobacterium Species Isolated from Decaying Biomass

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