Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa

Shukla, Shubham; Goyal, Arun

doi:10.1590/s1516-89132011000600006

Cited by 18 publications

(10 citation statements)

References 25 publications

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“…The similar results can also be found for other dextranproducing strains such as NRRL B-640 in research of Purama and Goyal [29]. The lower concentration of sucrose (5%) in the growth medium was the optimal for DS production by Weissella confusa Cab3 [30] and Lactobacillus plantarum DM5 [31].…”

Section: Effects Of Culture Medium Composition On Dextransucrase Prodsupporting

confidence: 82%

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Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Miljkovic¹,

Davidovic²,

Kralj

et al. 2017

Hem Ind

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The production of dextransucrase (DS) by Leuconostoc mesenteroides T3, novel isolate from water kefir grain, was studied and optimized. Bacterial supernatant reached activity of 3.1 U/ml when the culture was grown at 23 °C and under static culture condition using classical Tsuchiya medium for DS production. The increase of sucrose concentration to 7% led to an increase of DS activity by 52% compared to the control. Medium with 2% beef extract and 1% yeast extract resulted in 4.52 U/ml, which was 47% higher than in the control (with 2% yeast extract). Finally, the increase of K 2 HPO 4 concentration from 2 to 3% resulted in the increased enzyme activity by 28%. Enzyme purified by polyethylene glycol 400 fractionation displayed maximum activity at 30 °C and pH 5.4. Zymogram analysis confirmed the presence of DS of approximately 180 kDa. The addition of divalent cations Ca 2+ , Mg 2+ , Fe 2+ and Co 2+ led to a minor increase of DS activity, while the addition of Mn 2+was the most prominent with 73% increase. These findings classify dextransucrase from Leuconostoc mesenteroides T3 as promising candidate for production of dextran, which has numerous applications in various industries.Lactic acid bacteria (LAB) are one of the main classes of microorganisms that are known to produce several industrially important biomolecules among which exopolysaccharides (EPS) have the widest range of uses [1]. Fermentation processes for EPS production are quite expensive due to purification costs, giving an advantage to the use of enzymes. Current challenges in the LAB enzymes production include both strain improvement and enhancement of enzyme production.Dextransucrase (DS) is an extracellular enzyme that belongs to the class of glucosyltransferases [2] and it is mainly produced by microorganisms belonging to the families Lactobacillaceae and Streptococcaceae, especially by the genera Lactobacillus, Leuconostoc and Streptococcus [3]. Enzyme DS catalyzes the transfer reaction of glucosyl residues from sucrose to dextran polymer chain and releases fructose [4,5]. In the presence of appropriate acceptor (glucose, maltose, isomaltose, etc.) this enzyme can also produce oligosaccharides [6].

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Section: Effects Of Culture Medium Composition On Dextransucrase Prodsupporting

confidence: 82%

“…The optimum pH for DS production from our strain is in correlation with other reports for glucansucrases from various lactic acid bacteria viz. L. mesenteroides NRRL B-640 [29], W. confusa Cab3 [30] and Lb. plantarum DM5 [31].…”

Section: Optimum Ph and Ph Stability Of Dextransucrasementioning

confidence: 99%

Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Miljkovic¹,

Davidovic²,

Kralj

et al. 2017

Hem Ind

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“…Weissella confusa Cab3 grew well within the range of pH 5.0 to 7.0, and temperature 20˚C to 45˚C however Weissella confusa Cab3 produced maximum dextransucrase (6.0 U/ml) at pH 7.0 and 25˚C in the enzyme production medium [19]. As reported earlier, the effects of several medium components were investigated and sucrose, Tween80, yeast extract and K 2 HPO 4 were effective nutrients which displayed higher dextransucrase production [20]. Based on the earlier results, L-16 Taguchi's orthogonal array method was designed, where the factors were varied in four levels for determining optimal medium components for dextransucrase production from Weissella confusa Cab3.…”

Section: Resultsmentioning

confidence: 60%

“…To achieve the maximum dextransucrase production by Weissella confusa Cab3, sucrose (%, w/v), Yeast extract (%, w/v), dipotassium hydrogen orthophosphate (K 2 HPO 4 ; %, w/v) and Tween80 (%, v/v) were selected for medium optimization for enhanced dextransucrase production because they had significant impact on dextransucrase production as screened in our earlier findings [20]. Sucrose was the most effective medium component for dextransucrase production.…”

Section: Dextransucrase Activity Assaymentioning

confidence: 99%

“…Weissella confusa Cab3 produced dextransucrase (6.0 U/ml) in medium described by Tsuchiya et al, 1952. Further the effects of various carbon sources, nitrogen sources and buffering agents were investigated for their effects on dextransucrase and dextran production from Weissella confusa Cab3 [20]. In the present study the medium components were optimized for the enhanced dextransucrase production by Weissella confusa Cab3 using Taguchi's orthogonal method.…”

Section: Introductionmentioning

confidence: 99%

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Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Shukla¹,

Goyal²

2012

AiM

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The influence of medium ingredients on extracellular dextransucrase production by a new bacterial strain Weissella confusa Cab3 (Genbank Accession Number JX649223) was evaluated using fractional factorial design of Taguchi's orthogonal array. Four metabolism influencing factors viz. sucrose, yeast extract, K 2 HPO 4 and Tween80 were selected to optimize dextransucrase production by W. confusa Cab3 using fractional factorial design of Taguchi methodology. Based on the influence of interaction components of fermentation, least significant factors of individual level have higher interaction severity index and vice versa for enzyme production from Weissella confusa Cab3. Sucrose and yeast extract were found to be the most significant factors which positively influenced the dextransucrase production. The optimized medium composition consisted of sucrose-5%; yeast extract-2%; K 2 HPO 4 -1.0%; Tween80-0.5, based on Taguchi orthogonal array method. The optimized composition gave an experimental value of dextransucrase activity of 17.9 U/ml at shake flask level which corresponded well with the predicted value of 17.54 U/ml by the model. The optimized medium by Taguchi method gave significant (3 fold) enhancement of dextransucrase activity as compared to unoptimised enzyme activity of 6.0 U/ml. The dextransucrase production was scaled up in lab scale bioreactor resulting in further enhancement of enzyme activity (22.0 U/ml).

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Rye bran as fermentation matrix boosts in situ dextran production by Weissella confusa compared to wheat bran

Kajala

Mäkelä

Coda

et al. 2015

Appl Microbiol Biotechnol

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The consumption of fiber-rich foods such as cereal bran is highly recommended due to its beneficial health effects. Pre-fermentation of bran with lactic acid bacteria can be used to improve the otherwise impaired flavor and textural qualities of bran-rich products. These positive effects are attributed to enzymatic modification of bran components and the production of functional metabolites like organic acids and exopolysaccharides such as dextrans. The aim of this study was to investigate dextran production in wheat and rye bran by fermentation with two Weissella confusa strains. Bran raw materials were analyzed for their chemical compositions and mineral content. Microbial growth and acidification kinetics were determined from the fermentations. Both strains produced more dextran in rye bran in which the fermentation-induced acidification was slower and the acidification lag phase longer than in wheat bran. Higher dextran production in rye bran is expected to be due to the longer period of optimal pH for dextran synthesis during fermentation. The starch content of wheat bran was higher, which may promote isomaltooligosaccharide formation at the expense of dextran production. W. confusa Cab3 produced slightly higher amounts of dextran than W. confusa VTT E-90392 in all raw materials. Fermentation with W. confusa Cab3 also resulted in lower residual fructose content which has technological relevance. The results indicate that wheat and particularly rye bran are promising matrices for producing technologically significant amounts of dextran, which facilitates the use of nutritionally valuable raw bran in food applications.

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Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa

Cited by 18 publications

References 25 publications

Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3

Rye bran as fermentation matrix boosts in situ dextran production by Weissella confusa compared to wheat bran

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Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa

Cited by 18 publications

References 25 publications

Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Characterization of dextransucrase from Leuconostoc mesenteroides T3, water kefir grains isolate

Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from &lt;i&gt;Weissella confusa&lt;/i&gt; Cab3

Rye bran as fermentation matrix boosts in situ dextran production by Weissella confusa compared to wheat bran

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Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production from <i>Weissella confusa</i> Cab3