Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata

Esmaelizad, Majid; Niaraki, Saber Jelokhani; Fesharaki, Reza Hashemi

doi:10.1590/s1516-89132011000600005

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…However, sequencing and phylogenetic analyses of the Egyptian T. annulata showed that theTams-1 sequences are relatively diverse (87.8-100% identity values), dispersing themselves across several clades in the phylogenetic tree containing sequences from other countries [ 2 ]. Moreover, Tams1 diversity has been reported as being generated by the random mutation of nucleotides during asexual reproduction as well as by the selection of changes that confer a biological advantage instead of the differential expression of the members of a gene family [ 7 , 8 ].…”

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confidence: 99%

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Experimental immunology Genetic diversity and phylogenetic analysis of Tams1 of Theileria annulata isolates from three continents between 2000 and 2012

Wang¹,

Yang²,

Wang³

et al. 2014

cejoi

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Theileria annulata, which is part of the Theileria sergenti/Theileria buffeli/Theileria orientalis group, preferentially infects cattle and results in high mortality and morbidity in the Mediterranean, Middle East, and Central Asia. The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of T. annulata that could be used as a marker for epidemiological studies and phylogenetic analysis. In the present study, a total of 155 Tams1 sequences were investigated for genetic diversity and phylogenetic relationships through phylogenetic analysis. Results showed that the Tams1 sequences were divided into two major groups and that distribution for some isolates also exhibited geographic specificity. As targeting polymorphic genes for parasite detection may result in underestimation of infection, polymerase chain reaction (PCR) assay using two different probes targeting tams-1 genes of these two groups can be more credible. In addition, the direction of the spread of the disease was discovered to be from the Mediterranean or the tropical zone to the Eurasian peninsula, Middle East, Southern Asia, and Africa, particularly for Group 2. A similar occurrence was also found between the Ms1 gene of Theileria lestoquardi and the Tams1 gene of T. annulata, which explains cross-immunogenicity to a certain extent. However, no potential glycosylation site in the Tams1 of T. annulata was found in this study, which illustrated that instead of N-glycosylation, other modifications have more significant effects on the immunogenicity of the Tams1 protein.

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Section: Introductionmentioning

confidence: 99%

“…Several attempts have been made to analyze the phylogenetic characteristics of the Tams1 gene of T. annulata [ 8 ]. However, no distinct classification for all reported Tams1 sequences obtained from three continents (Asia, Africa, and Europe) is yet available.…”

Section: Introductionmentioning

confidence: 99%

Experimental immunology Genetic diversity and phylogenetic analysis of Tams1 of Theileria annulata isolates from three continents between 2000 and 2012

Wang¹,

Yang²,

Wang³

et al. 2014

cejoi

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“…Furthermore, OT in small ruminants causes a high morbidity rate reach to 100%, with a mortality rate of more than 46% (Tageldin et al, 2005;El Imam et al, 2015). There are several genes used for molecular detection of the T. ovis and T. lestoquardi such as Major merozoite piroplasm surface antigen (MMPSA) gene (Esmaelizad et al, 2011), 16S ribosomal ribonucleic acid (16S rRNA) gene (Ismael and Omer, 2020), 12S ribosomal ribonucleic acid (12S rRNA) gene (Azmi et al, 2019), small subunit ribosomal RNA (SSU rRNA) gene (Benedicto et al, 2020;Ceylan et al, 2021), and the more common gene is 18S ribosomal ribonucleic acid (18S rRNA) (Eliwa et al, 2021;Metwally et al, 2021). Ovine theileriosis can be diagnosed based on polymerase chain reaction (PCR) assay, it is an important technology in epidemiological and phylogenetic studies of Theileria spp.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic study of theileria ovis and theileria lestoquardi in infected sheep and it is associated ticks in mosul city, Iraq

Hamid

Al-Obaidi

2022

ijhs

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This is a first molecular report investigate the phylogenic analysis of Theileria spp. in sheep and it's infested ticks in Mosul city-Iraq. A total of 185 blood samples were collected from sheep in different areas of Mosul city. A sixty five Ixodid ticks were also collected from different parts of infected animals. The overall prevalence of Theileria spp. was 52.4% (97 out of 185), for Theileria ovis it was 9.7%, Theileria lestoquardi it was 20% and mixed infection it was 22.7% in sheep in Mosul city, based on conventional-PCR and multiplex-PCR techniques. the infestation rat of Ixodid ticks on sheep was 11.8% (22 out of 185) and three species of Ixodid ticks (n=65) were identified and classified: Hyalomma anatolicum anatolicum 37 (56.9%), Rhipicephalus sanguineus 16 (24.6%), Rh. turanicus 12 (18.4%) based on microscopic examination. BLASTn individual sequencing analysis of six sequences of 18S rRNA gene, composing sequences of T. ovis (n=3) (One extracted from sheep blood and two extracted from engorged female ticks), and sequences of T. lestoquardi (n=3) (One extracted from sheep blood and two extracted from engorged female ticks).

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Genetic diversity in the Tams1 gene of Theileria annulata (Duschunkowsky and Luhs, 1904) infecting cattle

et al. 2021

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Molecular and phylogenetic analysis of the partial tams1 gene sequence of a vaccine strain of Theileria annulata

Abstract: ABSTRACT

Cited by 6 publications

References 15 publications

Experimental immunology Genetic diversity and phylogenetic analysis of Tams1 of Theileria annulata isolates from three continents between 2000 and 2012

Experimental immunology Genetic diversity and phylogenetic analysis of Tams1 of Theileria annulata isolates from three continents between 2000 and 2012

Phylogenetic study of theileria ovis and theileria lestoquardi in infected sheep and it is associated ticks in mosul city, Iraq

Genetic diversity in the Tams1 gene of Theileria annulata (Duschunkowsky and Luhs, 1904) infecting cattle

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