Effects of catuaba cristal® on the testis of wistar rats

Freitas, Karine Moura de; Costa, Kyvia Lugate Cardoso; Campos, P.K.A.; Melo, Fabiana Cristina Silveira Alves de; Paula, Tarcízio Antônio Rêgo de; Matta, Sérgio Luís Pinto da

doi:10.1590/s1516-89132011000500007

Cited by 2 publications

(4 citation statements)

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“…Volume densities of testicular components (GE, L, and I) were determined by randomly superimposing a transparent grid comprising 160 test points per image arranged in a quadratic array (Freitas et al ., ). The ratio of the number of point intersections on the grid overlying each tissue component (P N ) to the total number of the points of the grid (P T ) was considered as the volume density (V V ) of the component, with the formula (Bielli et al ., ):

{normalV}_{normalV} = {normalP}_{normalN} / {normalP}_{normalT}

…”

Section: Methodsmentioning

confidence: 97%

Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy

et al. 2018

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The consumption of alcohol by people living with HIV/AIDS is associated with a graver prognosis. Long-term use of antiretrovirals may have certain health challenges that may be aggravated by concomitant alcohol use. This study investigated virgin coconut oil (VCO) as an adjuvant to the deleterious effects of highly active antiretroviral therapy (HAART) and alcohol on the cyto-architecture and functioning of the testis. Forty adult male Sprague-Dawley rats, weighing 165~176 g, were divided into eight groups and treated according to protocol. Testicular histology, stereological parameters, seminal fluid, testosterone, luteinizing hormone, follicle-stimulating hormone, the antioxidants marker malondialdehyde (MDA), and antioxidant glutathione (GSH) were examined. The use of ethanol alone and ethanol + HAART showed extensive degeneration in the seminiferous epithelium, decreased semen quality, disorganized basement membrane and widened, hypocellular interstitium. GSH was significantly decreased in the ethanol alone treated group with no significant effect on testosterone, LH, and MDA levels. Adjuvant treatment with VCO at low dose (2.5 mL/kg/bw) improved sperm motility with a partial restoration of the histopathological alterations. High doses of VCO (5.0 mL/kg/bw) showed greater improvement with respect to sperm counts, increased FSH hormonal and GSH antioxidant levels, and a well-preserved testicular cyto-architecture.

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{normalV}_{normalV} = {normalP}_{normalN} / {normalP}_{normalT}

…”

Section: Methodsmentioning

confidence: 97%

Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy

et al. 2018

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show abstract

“…Testicular fragments were dehydrated and embedded in hydroxyethyl methacrylate (Historesin ® , Leica). Sections (3 µm thick) were obtained and stained with toluidine blue/1% sodium borate (Monteiro et al 2008;Costa et al 2011;Freitas et al 2011;Gomes et al 2011). …”

Section: Euthanasia Tissue Collection and Biometrymentioning

confidence: 99%

“…All the following measurements were made according to previous studies Monteiro et al 2008;(Leite et al 2010;Predes et al 2010;Costa et al 2011;Freitas et al 2011). The images were captured with an Olympus-AX70 light microscope and the morphometrical and stereological analyses were performed using Image-Pro Plus 4 software.…”

Section: Morphometry and Stereologymentioning

confidence: 99%

“…Testicular density is around 1 (França 1991), thus, the testicular parenchyma volume had the same value as its weight. Tubule-somatic index (TSI) was calculated by the ratio between the seminiferous tubule volume and body mass, the result being multiplied by 100 Freitas et al 2011;Gomes et al 2011). The tubular diameter was measured in 10 seminiferous tubule sections with circular transections.…”

Section: Morphometry and Stereologymentioning

confidence: 99%

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