Blood glucose regulation during fasting in rats under food restriction since birth

Vitoriano, Adriana de Souza; Malta, Ananda; Barrena, Helenton C.; Furlan, Maria Montserrat Diaz Pedrosa; Bazotte, Roberto Barbosa; Gazola, Vilma Godoi

doi:10.1590/s1516-89132011000100009

Cited by 9 publications

(15 citation statements)

References 23 publications

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“…Consistent with this idea, higher glycogen content and similar findings on liver glucose metabolism were reported in reduced-litter, calorically-restricted rats aging 90 days [31]. As rats from large litters subjected to caloric restriction after weaning also display these patterns of liver glucose metabolism [24,32,33], it can be assumed that caloric restriction had an impact on liver glucose metabolism that seems to be dependent of the prevailing nutritional condition, and not of age or litter size.…”

Section: Discussionsupporting

confidence: 69%

Rat Hepatocyte Glucose Metabolism Is Affected by Caloric Restriction but not by Litter Size Reduction

Branquinho¹,

Cruz²,

Silvério³

et al. 2017

JPP

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Abstract:This work aimed at investigating whether the liver glucose metabolism could have a role in any change of glucose homeostasis that might exist in the reduced-litter rat at the age of 60 days. Additionally, post-weaning caloric restriction and its hepatic effects were explored, as this intervention is reported as a useful measure against obesity and its related disturbances. The animals were raised in litters of nine (control, CG) or three pups during lactation. These small-litter pups were FG (fed freely) or subjected to 30% RG (caloric restriction) after weaning until 60 days of age. The increased adiposity induced by lactational overfeeding was not reversed by caloric restriction. Hepatocyte glucose metabolism and glucose tolerance test were not affected by litter size, but caloric restriction increased liver basal glucose release, diminished gluconeogenesis and retarded the glycemic decay during the insulin tolerance test. Liver glucose metabolism of young adult rats was not affected by lactational overfeeding. Up to this age, however, moderate caloric restriction had a potent influence that might compromise whole-body glucose homeostasis and prompt to insulin resistance.

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Section: Discussionsupporting

confidence: 69%

Rat Hepatocyte Glucose Metabolism Is Affected by Caloric Restriction but not by Litter Size Reduction

Branquinho¹,

Cruz²,

Silvério³

et al. 2017

JPP

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“…In those studies, rats from 6-pups litters fed ad libitum after lactation had decreased glucose output and enhanced gluconeogenesis during in situ liver perfusion after overnight fasting, as expected, because fasting decreases liver glycogen stores and favors gluconeogenesis (Rui, 2014). In contrast, rats under caloric restriction since birth (raised in 12-pups litters and food-restricted to 50% after lactation) had a completely different profile: high glucose output and almost undetected gluconeogenesis after overnight fasting Vitoriano et al, 2011), very similar to animals in post-prandial state (Babata et al, 2014). In another investigation, free feeding after caloric restriction (refeeding) decreased adrenaline-stimulated glycogenolysis after overnight fasting (Garcia et al, 2017), suggesting that the changes in glucose metabolism were not due to litter size (that is, lactational nutrition), but to the chronic feeding regimen and, as such, reversible.…”

Section: Introductionmentioning

confidence: 59%

“…Our research group has been reporting changes of liver glucose metabolism caused by caloric restriction since lactation Vitoriano et al, 2011;Babata et al, 2014;Garcia et al, 2017). In those studies, rats from 6-pups litters fed ad libitum after lactation had decreased glucose output and enhanced gluconeogenesis during in situ liver perfusion after overnight fasting, as expected, because fasting decreases liver glycogen stores and favors gluconeogenesis (Rui, 2014).…”

Section: Introductionmentioning

confidence: 70%

Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

et al. 2020

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It was previously reported that liver glucose metabolism in rats under caloric restriction differs from that of freely-fed rats. This study hypothesized that these changes (1) were related to the expression of hypothalamic neuropeptides involved in metabolic control, and (2) were not a residual effect of litter size. To those purposes, liver glucose metabolism and hypothalamic expression of the orexigenic neuropeptides NPY (neuropeptide Y) and AgRP (agouti gene-related peptide); and of the anorexigenic neuropeptides POMC (pro-opiomelanocortin) and CART (cocaineand amphetamine-related transcripts) were investigated. Male Wistar rats from two different litter sizes (G6 and G12, with 6 or 12 pups, respectively) were subjected to free feeding (GL, ad libitum), 50% caloric restriction (GR) or caloric restriction+ad libitum refeeding (GRL) until the age of 90 days. Biometric values were lower in GR than in GL, while in GRL they were totally or partially recovered. Blood glucose variation during the pyruvate tolerance test (PTT) was small in GR. During in situ liver perfusion, total, basal, and adrenaline-stimulated liver glucose outputs were high in GR, but additional glucose output in the presence of alanine was negligible. Refeeding (GRL) yielded values close to those of GL. Litter size did not consistently influence any of these variables. The expression of transcripts of the hypothalamic neuropeptides was responsive to feeding regimen, litter size and/or their interaction and differed from G6 to G12, while the metabolic changes of the liver were qualitatively equal in both GR. Therefore, the changes in glucose metabolism in the liver of rats under caloric restriction were not determined by either litter size or hypothalamic neuropeptide expression and were linked only to the prevailing feeding regimen of the adult animal.

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“…This demonstrates the presence in the extract of antihyperglycaemic compounds that could have resulted in a more marked biological effect at a dose of 500 mg/kg. The extract would have acted thus by delaying or blocking the intestinal absorption of glucose or by sensitizing peripheral tissues to capture glucose or store it primarily as glycogen [16,17] .…”

Section: Discussionmentioning

confidence: 99%

Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice

Atchou¹,

Lawson-Evi²,

Metowogo³

et al. 2020

J. Drug Delivery Ther.

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Crataeva adansonii DC. ssp. adansonii is a medicinal plant belong to Capparidaceae family, used traditionally by Togolese to treat diabetes mellitus. The aim of this study was to evaluate the antihyperglycaemic and antioxidant activities of the plant leaves. The effect of hydroethanolic extract of C. adansonii ssp. adansonii leaves was assessed in vivo on blood glucose levels in fasting normal and glucose loaded hyperglycemic (oral glucose tolerance test) mice. The phytochemical screening has been done by coloring tests in chemistry. In vitro antioxidants assays had been performed by 2, 2'-azobis 2 amidinopropane dihydrochloride (AAPH) free radical scavenging assay, Ferric reducing antioxidant power (FRAP) assay, Fe2+ chelating assay and total antioxidant capacity (TAC) quantification. After 30 min glucose loading (4 g/kg), the extract 500 mg/kg was showed a significant (p < 0.001) decreased of the peak of blood glucose compared to the hyperglycemic control. The two doses of extract (250 and 500 mg/kg) did not act on the basic blood glucose compared to the normoglycemic control. Phytochemical screening revealed a presence of flavonoids, tannins, alkaloids and sterols. AAPH free radical scavenging activity, reducing power, Fe2+ chelating activity and total antioxidant capacity have shown dose-dependent antioxidant capacity and reducing power of extract compared to ascorbic acid and Butylated hydroxyl Toluene used as reference drug. These finding prove that C. adansonii ssp. adansonii leaves can be used in the treatment of diabetes mellitus and its complications related to oxidative stress and support the use of the plant in traditional medicine in diabetes treatment. Keywords: Crataeva adansonii, antihyperglycaemic, phytochemical, antioxidant.

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Blood glucose regulation during fasting in rats under food restriction since birth

Cited by 9 publications

References 23 publications

Rat Hepatocyte Glucose Metabolism Is Affected by Caloric Restriction but not by Litter Size Reduction

Rat Hepatocyte Glucose Metabolism Is Affected by Caloric Restriction but not by Litter Size Reduction

Differential Responses of Liver and Hypothalamus to the Nutritional Condition During Lactation and Adult Life

Antihyperglycaemic and antioxidant activities of Crataeva adansonii DC. ssp. adansonii leaves extract on ICR mice

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