Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis

Pagnoncelli, Maria Giovana Binder; Araújo, Nathália Kelly de; Silva, Nayane Macedo Portela da; Assis, Cristiane Fernandes de; Rodrigues, Sueli; Macêdo, Gorete Ribeiro de

doi:10.1590/s1516-89132010000600023

Cited by 28 publications

(17 citation statements)

References 28 publications

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“…Unlike αGI, there are few reports of chitinolytic enzymes and proteases being produced by Paenibacillus species. Chitosanase produced by Paenibacillus ehimensis NRRL B-23118 has been studied, but purification was not performed [ 33 ]. To compare the different chitinases and proteases produced by Paenibacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

et al. 2018

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Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources.

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Section: Resultsmentioning

confidence: 99%

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

et al. 2018

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“…Chitosan (1%) was treated with1M acetic acid and adjusted to pH 5 using 2M sodium acetate before its use as a substrate for enzyme assay [14]. The enzyme activity was determined according to Pagnoncelli et al, [15] by mixing 500µL of the clear culture filtrate with 500µL of 1% soluble chitosan and the reaction mixture was incubated at 50°C for 30min. At the end of the assay time, 2.5mL of DNS was added to stop the reaction.…”

Section: Enzyme Assaymentioning

confidence: 99%

Optimization of Dothideomycetes sp. css035 chitosanase productivity and activity using response surface methodology

Hashem

Hosny

Awad³

et al. 2018

Egypt. J. Chem.

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A MONG the methods used for the hydrolysis of chitosan, enzymatic hydrolysis using chitosanase was selected in order to produce high yield of specific chitooligosaccharides with less environmental pollutions. The production of Dothideomycetes sp. NRC-SSW extracellular chitosanase was statistically optimized in which a two -phase experimental design was applied. Plackett -Burman design was used to evaluate the relative importance of culture conditions and medium components for chitosanase production. Chitosan concentration, agitation speed and incubation period were found to be the most significant variables that affected the chitosanase production and their optimal values were obtained by applying Box-Behnken design. The optimized medium composed of (g/L) chitosan, 30; K 2 HPO 4 , 1.5; MgSO 4 , 0.4; KCl, 4.0; yeast extract, 18.5 and FeSO 4 ,0.01; at pH 5.5, 30°C and 180rpm for 96h gave 13.9U/mL with 36.3% increase in the activity. The R 2 value was 0.954 and this indicated the aptness of the model. The optimization of the hydrolytic conditions required for chitooligosaccharides production was also performed by Box-Behnken design. The highest yield of chitooligosaccharides was obtained with enzyme/ substrate ratio 0.05U/mg in 0.2M Tris HCl buffer incubation at 60 ο C for 5h. The cytotoxic activity of the chitooligosaccharides was tested in vitro against Hep-G2 and MCF7.

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“…The genus Paenibacillus has been reported for production of several industrially important enzymes such as amylase, cellulase, hemicellulase, pectinase, lignin‐modifying enzymes , chitosanase , xylan‐degrading enzymes , 4,6‐α‐glucanotransferase enzymes . The enzymes have been used for many applications in industries such as biofuel, paper, textiles, detergents, food and feed, as well as healthcare.…”

Section: Introductionmentioning

confidence: 99%

Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain

Lincoln

More²,

2019

J Basic Microbiol

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In the present study, glucoamylase produced from a soil bacterium Paenibacillus amylolyticus NEO03 was cultured under submerged fermentation conditions. The extracellular enzyme was purified by starch adsorption chromatography and further by gel filtration, with 2.73‐fold and recovery of 40.02%. The protein exhibited molecular mass of ∼66,000 Da as estimated by SDS–PAGE and depicted to be a monomer. The enzyme demonstrated optimum activity at pH range 6.0–7.0 and temperature range 30–40 °C. Glucoamylase was mostly activated by Mn2+ metal ions and depicted no dependency on Ca2+ ions. The enzyme preferentially hydrolyzed all the starch substrates. High substrate specificity was demonstrated towards soluble starch and kinetic values Km and Vmax were 2.84 mg/ml and 239.2 U/ml, respectively. The products of hydrolysis of soluble starch were detected by thin layer chromatography which showed only D‐glucose, indicating a true glucoamylase. The secreted glucoamylase from P. amylolyticus strain possesses properties suitable for saccharification processes such as biofuel production.

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Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis

Abstract: The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v)

Cited by 28 publications

References 28 publications

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

Optimization of Dothideomycetes sp. css035 chitosanase productivity and activity using response surface methodology

Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain

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