Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus

Abstract

ABSTRACT

Efficient ethanol production from inulin by two-stage aerate strategy

“…Inulinase activity, the most important factor for the process of CBP, can be influenced by aeration rates and biomass concentration at the same time [15,16]. Unfortunately, it was paid little concerned during the ethanol fermentation [6,13,17,18].…”

<b>Agave syrup as a substrate for inulinase production by <i>Kluyveromyces marxianus</i> NRRL Y-7571

“…At pH values above 6.0, the activity declined, and at alkaline pH (8.0 to 10.0), the enzyme activity was close to zero. Other authors have also reported these pH and temperature optimums for inulinase produced by K. marxianus Cazetta et al, 2010;Risso et al, 2010). Inulinases of other fungal genera feature similar biochemical characteristics, such as the enzymes from Pichia guilliermondii (Chi et al, 2009), Geotrichum candidum (Erdal et al, 2011), Aspergillus ochraceus (Guimarães et al, 2007), and A. niger (Dinarvand et al, 2012;Yewale et al, 2013).…”

“…In both factorial designs, high concentrations of agave syrup resulted in a decrease of enzyme production, which could be related to catabolic repression. The production of inulinase is described to suffer from catabolic repression at high substrate concentrations, and therefore, the highest production of this enzyme is usually observed at the end of the growth phase (Parekh & Margaritis, 1985;Jing, Zhengyu, & Augustine, 2003;Cazetta, Monti, & Contiero, 2010;Singh & Lotey, 2010). Table 5 shows, through regression coefficients, that both agave syrup (p = 0.02) and yeast extract (p = 0.04), in their quadratic terms, negatively affected the enzyme production.…”

Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract

“…Influence of sucrose concentration and culture conditions (pH and aeration rate) were studied on inulinase production by K. marxianus var. bulgaricus ATCC 16045 to obtain maximum yield of 15.29 U mL -1 (2). More recently, optimization of agro-industrial medium comprised of sugarcane bagasse, molasses and Corn steep liqour has been attempted to maximize the production of inulinase upto 436 U/gds (9).…”