Effect of deoxynivalenol and T-2 toxin in malt amylase activity

Garda‐Buffon, Jaqueline; Baraj, Edlira; Badiale‐Furlong, Eliana

doi:10.1590/s1516-89132010000300002

Cited by 18 publications

(8 citation statements)

References 23 publications

(17 reference statements)

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“…The protein extracts from oat, rice and wheat contained 0.52; 0.60 and 0.40 mg of protein ml -1 , respectively. The mixture was incubated at 25 ºC for 30 minutes, followed by the addition of soluble starch solution 0.5% (1 ml) and incubated for 30 minutes at the same temperature (Baraj, Garda-Buffon, & Badiale-Furlong, 2010). In the control experiment, the extracts were substituted by water.…”

Section: Specificity Of the Protein Inhibitor Extractmentioning

confidence: 99%

“…The effect of the temperature was investigated by incubating the reaction mixture containing buffer pH 5, at temperatures of 25ºC, 35ºC, 45ºC and 60ºC for 30 minutes. Thermal stability was assessed at the same temperatures, with prior incubation of the enzymatic solution in buffer for 30, 60 and 90 minutes before the addition of substrate for evaluating enzymatic activity (Baraj, Garda-Buffon, & Badiale-Furlong, 2010).…”

Section: Biochemical Characterization Of Commercial Fungal Amylasementioning

confidence: 99%

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Procedure to Determine Enzyme Inhibitors Activity in Cereal Seeds

Pagnussatt¹,

Meza²,

Garda‐Buffon³

et al. 2012

JAS

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This work established a procedure for commercial fungal amylase usage as indicator of enzyme inhibitors presence in cereals, intending to screening antifungal resistance properties in cereals. Firstly, the inhibitive effects of oat, wheat and rice protein extracts were assessed against different amylase sources. It was found that the fungal amylase (Fungamyl ® ) was the most affected by the inhibition property of the extracts. The best conditions for inhibitor-extract-enzyme interaction were established (pH, temperature, starch concentration). Inhibition kinetic allowed their classification as mixed inhibitors. A high quocient (1.7) was observed between enzyme inhibition and Fusarium graminearum multiplication reduction. Fungamyl ® amylase demonstrated its potential to detect the protein inhibitor presence in different cereals and might be employed in screening natural resistance to fungal contamination.

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Section: Specificity Of the Protein Inhibitor Extractmentioning

confidence: 99%

Section: Biochemical Characterization Of Commercial Fungal Amylasementioning

confidence: 99%

Procedure to Determine Enzyme Inhibitors Activity in Cereal Seeds

Pagnussatt¹,

Meza²,

Garda‐Buffon³

et al. 2012

JAS

Self Cite

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“…α‐Amylase activity was assessed by starch degradation, which was quantitatively estimated by the iodometric method. The spectrophotometric absorbance of the resulting complex at 620 nm was recorded (Garda‐Buffon, Baraj, & Badiale‐Furlong, ).…”

Section: Methodsmentioning

confidence: 99%

Profile of Phenolic Compounds Released from Rice Bran by Rhizopus oryzae and Trichoderma reesei: Their Relation with Hydrolases Activity

Souza

Leal

Massarolo

et al. 2019

Journal of Food Science

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Evolution of Rhizopus oryzae and Trichoderma reesei biomass in rice bran, their enzyme activity, and the profile of phenolic compounds released from the lignocellulosic matrices were determined and correlated by principal component analysis (PCA). PCA analysis confirms that cultivation of rice bran affected the release of methanol‐soluble phenolic compounds (MSPC), ethanol‐soluble phenolic compounds (ESPC), and bound phenolic compounds (BPC) positively, due to their enzymatic activity. The release of MSPC was influenced by the activity of cellulase and endoglucanase, which increased 110.6% and 136.3%, respectively, for Rhizopus oryzae and Trichoderma reesei. Gallic acid was the main component in the MSPC and ESPC compound fractions. Ferulic and syringic acids were found in its bound (BPC) form in the biomass. This study showed that bioactive compounds be released from lignocellulosic materials by fungus action and this process can be conducted to obtain specific phenolic compounds. Practical Application Due the demand by natural compounds with biological activity, such as phenolic compounds, it is interesting to purpose alternatives to enhance their yield, like for instance, by fungal fermentation of lignocellulosic material. Therefore, understanding the relations among different phenolic compounds released and the production of fungal hydrolases during growth of Rhizopus oryzae and Trichoderma reesei in solid state cultivation using rice bran as a substrate is fundamental to control the process. This knowledge gets viable scale up to apply the phenolic compounds as preservative in food chain, because this becomes possible directing the process to obtain specific bioactive compounds in less time of cultivation and with low cost.

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“…A tecnologia do uso de inibidores de enzimas digestivas como fator de resistência, tem chamado a atenção de vários pesquisadores 3,4,5 A inibição do crescimento micelial do patógeno foi avaliada durante os 7 dias, medindo o diâmetro da colônia do fungo, com 3 medições em sentidos perpendicularmente opostos, nos experimentos com extratos inibidor e nos controles. O conteúdo de glicosamina 6 e a inibição da atividade da enzima α amilase também foram determinados para avaliar a ação dos extratos 7 . As inibições foram calculadas através da diferença entre os cultivos tratados e os controles.…”

Section: Introductionunclassified

Ação antifúngica de inibidores de alfa amilase extraídos de trigo (Triticum aestivum L.)

Mendes

Alves

Cavalheiro

et al. 2013

BBR - Biochem. Biotechnol.

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RESUMO Neste trabalho foram extraídos inibidores enzimáticos de diferentes variedades de trigo os quais foram aplicados em cultivo "in vitro INTRODUÇÃOOs fungos do gênero Fusarium são encontrados em plantas e em resíduos de culturas por todo o mundo, sendo responsáveis pela infecção e destruição de culturas economicamente relevantes como o milho, trigo e outros pequenos grãos. Dentre estes fungos encontram-se espécies toxigênicas, como o Fusarium verticillioides 1 , o qual tem recebido atenção de agências regulatórias, como a ANVISA, por ser um potencial produtor de Fumonisina B 1 .As medidas químicas e agronômicas para o controle de F. verticillioides não são muito eficazes, pois a espécie por ser endofítica, coloniza as plantas sem demonstrar qualquer sinal da infecção. O uso de fungicidas sintéticos é a tecnologia mais comum para proteger as colheitas da deterioração fúngica e da contaminação por micotoxinas. No entanto, a maioria dos fungicidas sintéticos possui toxicidade residual e contribui para a seleção de espécies fúngicas resisitentes 2 . As plantas, por sua vez, possuem uma série de compostos que estão associados com a resistência à fatores bióticos e abióticos, como insetos e fungos. A tecnologia do uso de inibidores de enzimas digestivas como fator de resistência, tem chamado a atenção de vários pesquisadores 3,4,5 , pois as α amilases são enzimas essenciais para o crescimento e

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Effect of deoxynivalenol and T-2 toxin in malt amylase activity

Cited by 18 publications

References 23 publications

Procedure to Determine Enzyme Inhibitors Activity in Cereal Seeds

Procedure to Determine Enzyme Inhibitors Activity in Cereal Seeds

Profile of Phenolic Compounds Released from Rice Bran by Rhizopus oryzae and Trichoderma reesei: Their Relation with Hydrolases Activity

Ação antifúngica de inibidores de alfa amilase extraídos de trigo (Triticum aestivum L.)

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