Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Carone, Sílvia Bicudo; Santa–Catarina, Claudete; Silveira, Vanildo; Floh, Eny Iochevet Segal

doi:10.1590/s1516-89132010000200020

Cited by 3 publications

(1 citation statement)

References 38 publications

(53 reference statements)

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“…We predicted that this approach would allow us to verify the putative involvement of some vital biochemical pathways, including the methionine cycle [53] and its crucial compound S-adenosyl-L-methionine (SAM), in DNA methylation [54], which could contribute to SV [19]. Moreover, we suspected that compounds indicative of stress caused by in vitro culture, such as lipids [55], polyamines [56], and polysaccharides [57], might also affect DNA methylation.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation patterns remains largely unknown. In this study, the methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach was used to investigate de novo methylation, demethylation, and sequence variation in barley regenerants derived by anther culture. Additionally, we used Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy to identify the spectral features of regenerants, which were then analyzed by mediation analysis. The infrared spectrum ranges (710–690 and 1010–940 cm−1) identified as significant in the mediation analysis were most likely related to β-glucans, cellulose, and S-adenosyl-L-methionine (SAM). Additionally, the identified compounds participated as predictors in moderated mediation analysis, explaining the role of demethylation of CHG sites (CHG_DMV) in in vitro tissue culture-induced sequence variation, depending on the duration of tissue culture. The data demonstrate that ATR-FTIR spectroscopy is a useful tool for studying the biochemical compounds that may affect DNA methylation patterns and sequence variation, if combined with quantitative characteristics determined using metAFLP molecular markers and mediation analysis. The role of β-glucans, cellulose, and SAM in DNA methylation, and in cell wall, mitochondria, and signaling, are discussed to highlight the putative cellular mechanisms involved in sequence variation.

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Section: Discussionmentioning

confidence: 99%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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In vitro organogenesis of Cedrela fissilis Vell. (Meliaceae): the involvement of endogenous polyamines and carbohydrates on shoot development

Aragão

Ribeiro

Reis

et al. 2015

Plant Cell Tiss Organ Cult

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Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential

Oliveira¹,

Navarro²,

Cerruti³

et al. 2018

Plant and Cell Physiology

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The mechanisms that control polyamine (PA) metabolism in plant cell lines with different embryogenic potential are not well understood. This study involved the use of two Araucaria angustifolia cell lines, one of which was defined as being blocked, in that the cells were incapable of developing somatic embryos, and the other as being responsive, as the cells could generate somatic embryos. Cellular PA metabolism was modulated by using 5 mM arginine (Arg) or ornithine (Orn) at two time points during cell growth. Two days after subculturing with Arg, an increase in citrulline (Cit) content was observed, followed by a higher expression of genes related to PA catabolism in the responsive cell line; whereas, in the blocked cell line, we only observed an accumulation of PAs. After 14 d, metabolism was directed towards putrescine accumulation in both cell lines. Exogenous Arg and Orn not only caused a change in cellular contents of PAs, but also altered the abundance of a broader spectrum of amino acids. Specifically, Cit was the predominant amino acid. We also noted changes in the expression of genes related to PA biosynthesis and catabolism. These results indicate that Arg and Orn act as regulators of both biosynthetic and catabolic PA metabolites; however, we suggest that they have distinct roles associated with embryogenic potential of the cells.

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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Abstract: The aim of this work was to determine PAs levels in pith tissues

Cited by 3 publications

References 38 publications

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

In vitro organogenesis of Cedrela fissilis Vell. (Meliaceae): the involvement of endogenous polyamines and carbohydrates on shoot development

Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential

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