Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells

Carneiro, Marcella Lemos Brettas; Porfírio, Elaine Paulucio; Otake, Andréia Hanada; Chammas, Roger; Báo, Sônia Nair; Guillo, Lidia Andreu

doi:10.1590/s1516-89132010000200013

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…The suspension of non-loaded nanocapsules (Nano_0) showed a liquid aspect with a slightly bluishwhite opalescent coloring as typically reported in literature [17]. However, the suspension of CUR-loaded PEG-PCL nanocapsules (Nano_CUR) presented a yellow liquid aspect due to the original intense yellow color of CUR [4].…”

Section: Preparation Of Polymeric Nanocapsulessupporting

confidence: 58%

“…CUR has been recommended for several diseases, such as cancer, neurological disorders, infections, inflammations, atherosclerosis, joint and liver diseases [1,2]. Clinical evidences have been showed that CUR presents antitumor effect against a wide diversity of cancer types, including gastrointestinal, melanoma, genitourinary, breast, lung, neck, and sarcoma [4]. In general, CUR acts by suppressing the initiation, the promotion, and the metastasis of tumor cells when used alone or in combination with other chemotherapeutic agents [5].…”

Section: Introductionmentioning

confidence: 99%

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PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line

Rudnik

Lyra

Barboza

et al. 2020

Braz. arch. biol. technol.

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Curcumin (CUR) shows potential use for treating cancer. However, CUR has low solubility and reduced bioavailability, which limit its clinical effect. Therefore, the development of nanocarriers can overcome these problems and can ensure the desired pharmacological effect. In addition, it is mandatory to prove the quality, the efficacy, and the safety for a novel nanomedicine to be approved. In that sense, this paper aimed (a) to prepare CUR-loaded polyethylene glycol-poly(ε-caprolactone) nanocapsules; (b) to validate an analytical method by high performance liquid chromatography (HPLC) for quantifying CUR in these nanoformulations; (c) to evaluate the physicochemical stability of these formulations; and to investigate their cytotoxicity on NIH-3T3 mouse fibroblast cells. The HPLC method was specific to CUR in the loaded nanocapsules, linear (r = 0.9994) in a range of 10.0 to 90.0 µg.mL-1 with limits of detection and quantification of 0.160 and 0.480 µg.mL-1 , respectively. Precision was demonstrated by a relative standard deviation lower than 5%. Suitable accuracy (102.37 ± 0.92%) was obtained. Values of pH, particle size, polydispersity index, and zeta potential presented no statistical difference (p > 0.05) for CUR-loaded nanoparticles. No cytotoxicity HIGHLIGHTS  A simple and low-cost HPLC method for determining curcumin in nanocapsules was validated.  PEG-PCL nanocapsules containing curcumin showed suitable stability over 60 days.  No cytotoxicity was observed against NIH-3T3 mouse embryo fibroblast cell line.  PEG-PCL nanocapsules containing curcumin can be further investigated for cancer treatment. 2 Rudnik, L.A.C.; et al.

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Section: Preparation Of Polymeric Nanocapsulessupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line

Rudnik

Lyra

Barboza

et al. 2020

Braz. arch. biol. technol.

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“…27 Many studies have shown that curcumin nanocomposites could lead to cell cycle arrest in G0/G1 in cancer cells. 57 But our study indicates that curcumin-LDH-PDT (100 µg/mL) can induce G0/G1 arrest in MDA-MB-231 breast cancer cells along with induction of apoptosis (Figures 4 and 5) and Finally, as it is known curcumin is a multiaction drug that targets different pathways inside the body. 57 But our study indicates that curcumin-LDH-PDT (100 µg/mL) can induce G0/G1 arrest in MDA-MB-231 breast cancer cells along with induction of apoptosis (Figures 4 and 5) and Finally, as it is known curcumin is a multiaction drug that targets different pathways inside the body.…”

Section: Discussionmentioning

confidence: 67%

“…56 It is interesting that G0/G1 cell cycle arrest has been reported mainly in normal curcumin-treated cells. 57 But our study indicates that curcumin-LDH-PDT (100 µg/mL) can induce G0/G1 arrest in MDA-MB-231 breast cancer cells along with induction of apoptosis (Figures 4 and 5) and F I G U R E 7 Effects of curcumin-layered double hydroxide (LDH) nanohybrid-photodynamic treatment (PDT) on intracellular reactive oxygen species generation in MDA-MB-231 cells. The cells were treated with 0 (fx2), 25 (fx3), 100 µg/ml (fx4) curcumin-LDH nanohybrid and then irradiated with blue light-emitting diode (PDT).…”

Section: Discussionmentioning

confidence: 83%

Photodynamic treatment with anionic nanoclays containing curcumin on human triple‐negative breast cancer cells: Cellular and biochemical studies

Khorsandi

Hosseinzadeh²,

Shahidi

2018

J of Cellular Biochemistry

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Photodynamic treatment is a minimally invasive and clinically approved procedure for eliminating selected malignant cells with activation of a photosensitizer agent at a specific light. Little is known, however, about the phototoxic properties of curcumin, as a natural phenolic compound, against different types of cancers. It is generally accepted that cellular damage occurs during photo treatment. There is a limitation in using of curcumin as a drug due to its low solubility, but nanoparticles such as anionic nanoclays or layered double hydroxide (LDH) could overcome it. The aim of this study was to investigate cellular responses to curcumin‐LDH nanoparticles after photodynamic treatment of MDA‐MB‐231 human breast cancer cells. For this purpose, the MDA‐MB‐231 human breast cancer cell line treated with curcumin‐LDH nanoparticle and then irradiated (photodynamic treatment). After irradiation, lactate dehydrogenase assay, clonogenic cell survival, cell death mechanisms such as autophagy and apoptosis were determined. Cell cycle distribution after photodynamic therapy (PDT) and also intracellular reactive oxygen species (ROS) generation were measured. The result showed that curcumin‐LDH–PDT has a cytotoxic and antiprolifrative effect on MDA‐MB‐231 human breast cancer cells. Curcumin‐LDH–PDT induced autophagy, apoptosis, and G0/G1 cell cycle arrest in human breast cancer cell line. Intracellular ROS increased in MDA‐MB‐231 cancer cell line after treatment with curcumin‐LDH along with irradiation. The results suggest that curcumin‐LDH nanoparticle could be considered as a novel approach in the photodynamic treatment of breast cancer.

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“…Vesicular structure of the mitochondrial inner membrane was altered which eventually leads to the loss of cisternae [47–50]. Ultrastructural images (Figure 6) suggest that CUR-MSNAP influenced cells to undergo apoptosis by remodelling the inner mitochondrial membrane from normal vesicular structure to swollen vesicular at 24 h and completely swollen mitochondria at 48 h. Interestingly, it has been reported that unbound curcumin induces apoptosis by damaging chromosome and the plasma membrane in MCF-7 cells, however, there is no report to suggest that unbound curcumin cause mitochondrial insult [51]. Lv et al reported free curcumin-induced apoptosis in breast cancer cells by mitochondrial insult with cisternae degradation at 48 h [52].…”

Section: Discussionmentioning

confidence: 99%

An ingenious non-spherical mesoporous silica nanoparticle cargo with curcumin induces mitochondria-mediated apoptosis in breast cancer (MCF-7) cells

et al. 2019

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Curcumin delivery to cancer cells is challenging due to its hydrophobic nature, low bio distribution and low availability. Many nano vehicles suffer from low stability and toxicity, and hence the prerequisite of a non-toxic nano vehicle with effective drug delivery is still being delved. The present study investigates the delivery efficiency of curcumin with non-spherical mesoporous silica nanoparticles (MSNAs). Their mechanism of drug delivery and signalling proteins activated to induce apoptosis was further explored in MCF-7 cells. A non-spherical MSN was synthesised, functionalised with PEI (MSNAP) and analysed its intracellular behaviour. Our result indicates that MSNAP was non-toxic until 20 µg/mL and likely localizes in cytoplasmic vesicles. On contrast, well-known MCM-41P induced autophagosome formation, indicating cellular toxicity. Curcumin was loaded on MSNAP and its effectiveness in inducing cell death was studied in MCF-7 and in MCF-7R cells. Curcumin loading on MSNAP induces better cell death with 30 µM curcumin, better than unbounded curcumin. Western blot analysis suggest, curcumin induce apoptosis through the activation of caspase 9, 6, 12, PARP, CHOP and PTEN. The cell survival protein Akt1 was downregulated by curcumin with and without the nanostructure. Interestingly, cleaved caspase 9 was activated in higher amount in nano-conjugated curcumin compared to the free curcumin. But other ER resident protein like IRE1α, PERK and GRP78 were downregulated indicating curcumin disturbs ER homeostasis. Further, electron microscopic analysis reveled that nanocurcumin induced apoptosis by disrupting mitochondria and nucleus. Our results with doxorubicin resistant MCF-7 cell lines confirm nanodelivery of doxorubicin and curcumin sensitised cells effectively at lesser concentration. Further docking studies of curcumin indicate it interacts with the apoptotic proteins through hydrogen bonding formation and with higher binding energy.

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Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells

Cited by 8 publications

References 28 publications

PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line

PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line

Photodynamic treatment with anionic nanoclays containing curcumin on human triple‐negative breast cancer cells: Cellular and biochemical studies

An ingenious non-spherical mesoporous silica nanoparticle cargo with curcumin induces mitochondria-mediated apoptosis in breast cancer (MCF-7) cells

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