Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells

Abstract

Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors.Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.

Optimization of Flavonoid Production in Plant Cell Culture of Thevetia peruviana Elicited with Methyl Jasmonate and Salicylic Acid

“…Similar phenomenon was reported by Mendoza [32]; lower concentrations of MeJa (2-5mg/L) showed significantly higher pigment production compared to the control [38]. The reason could be that there are biochemical or molecular alterations within the cell increase the secondary metabolites production such as phenolic compounds [39]. Isah [40] in his review established that the defense response system becomes activated when intra or extracellular signal is received by receptors in cell plasma membrane, involving their binding accompanied by signal transduction cascade initiation that may result in de novo synthesis or activation of transcription factors responsible for regulating secondary metabolites biosynthesis genes expression.…”