Development of a Low Cost Bioprocess for Endotoxin Production by Bacillus thuringiensis var israelensis Intended for Biological Control of Aedes aegypti

Soccol, Carlos Ricardo; Pollom, Teresinha E. V.; Fendrich, Ricardo Cancio; Prochmann, Fernando Alberto; Mohan, Radijiskumar; Blaskowski, Marita Maciel Moreira; Melo, André Luiz de Almeida; Carvalho, Cláudio José Barros de; Soccol, Vanete Thomaz

doi:10.1590/s1516-89132009000700017

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…thuringiensis toxins in the midgut epithelium is "uncoupler" oxidative phosphorylation in the mitochondria, so the effect of ion transport of K to be disturbed 4 . Bacillus thuringiensis application is cultured on medium contain soybean meal and sugarcane molasses lead to high toxic effectiveness against Aedes aegypti (Soccol et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

2019

Int. J. Biosci.

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Bacillus thuringiensis is an important biological control agent in nature. For farmers to utilize B. thuringiensis, it needs to be researched in media where it can be reproduced easily, quickly, and produce many cells with high pathogenicity. The study aimed to test the influence of some propagation media on the growth of B. thuringiensis Berliner cell populations that have a high pathogenicity and compares the pathogenicity between B. thuringiensis propagated in some media against Plutella xylostella caterpillars. Bacillus thuringiensis isolates were taken from the tidal land on the islands of Borneo. The research used a completely randomized design with five treatments a) J Media (corn extract; b) K Media (soybean extract); c) B Media (rice extract); d) C Media (extract mixture of corn, soybean, and rice ratio of 1: 1: 1); and e) Nutrien Broth Media (NB Media) and four replications. The parameter measured was the number of cells. Pathogenicity test between B. thuringiensis that is propagated in some media against P. xylostella caterpillars were determined by probit analysis. The results showed that, growth media that produced the most B. thuringiensis cells are J Media (corn extract), B Media (extract of rice), and C Media (extract mixture of rice, corn, and soybeans). The highest pathogenicity of B. thuringiensis which is propagated in the C Media (extract mixture of corn, rice and soybeans) with LC50 value is 7.96 × 10 5 cells/ml suspension. The media type which produce the most B. thuringiensis cells and have the highest pathogenicity is C media (extract mixture of corn, rice and soybeans).

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Section: Resultsmentioning

confidence: 99%

Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

2019

Int. J. Biosci.

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Utilisation of response surface methodology to optimise the culture medium forBacillus thuringiensissubsp.israelensis

Angelo

Vilas-Bôas

Castro-Gómez

et al. 2014

Biocontrol Science and Technology

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Técnicas de identificação e meios de cultivo para crescimento de Bacillus thuringiensis utilizados no controle de mosquitos vetores: Mini revisão

Viana

Lobo

Neta

et al. 2021

RSD

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Bacillus thuringiensis (Bt) é uma bactéria de interesse mundial, devido sua elevada toxicidade a uma ampla gama de insetos vetores de agentes patogênicos ao homem e pragas agrícolas. A ação inseticida de Bt é conferida pela presença de cristal proteico, que atuam como toxinas. Diversos estudos são realizados com intuito de selecionar linhagens de Bt de diferentes ecossistemas com ação mosquitocida para insetos vetores. Observa-se que o solo é o substrato mais utilizado para o isolamento de Bt, sendo amplamente encontrado na natureza, predominantemente, na forma de esporos. A busca de novas linhagens de Bt em diferentes regiões do mundo tem como objetivo obter novas toxinas com ação inseticida, e que possam ser utilizadas na produção de biopesticidas. Para o cultivo das linhagens, são utilizados os meios Ágar Nutriente ou NYSM. Os meios de cultura possuem grande variedade de nutrientes, sendo ricos em carbono, nitrogênio e sais minerais, que são utilizados para induzir o crescimento dos microrganismos. Portanto, diversos estudos buscam aprimorar esses meios para o crescimento de Bt, utilizando meios alternativos eficazes e econômicos, visando o controle de mosquitos vetores.

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Development of a Low Cost Bioprocess for Endotoxin Production by Bacillus thuringiensis var israelensis Intended for Biological Control of Aedes aegypti

Abstract: ABSTRACT

Cited by 4 publications

References 22 publications

Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

Bacillus thuringiensis berliner cells population growth in some naturally media and the patogenicity against Plutella xylostella Caterpilars

Utilisation of response surface methodology to optimise the culture medium forBacillus thuringiensissubsp.israelensis

Técnicas de identificação e meios de cultivo para crescimento de Bacillus thuringiensis utilizados no controle de mosquitos vetores: Mini revisão

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