Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b proteins of Anaplasma marginale

Tamekuni, Kátia; Vidotto, Marilda Carlos; Félix, Samuel Rodrigues; Igarashi, Michelle; Garcia, João Luís; Coelho, Adriana Letícia M; Gasparini, Marcela Ribeiro; Marana, Elizabete Regina Marangoni; Silva, Sergio Silva da; Vidotto, Odilon

doi:10.1590/s1516-89132009000700016

Cited by 1 publication

(2 citation statements)

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“…Therefore, the immunodominance of epitopes seems to be a key factor limiting the adaptive immunity [20] and this investigation was an opportunity to provide evidences that a core epitope-based vaccine approach is possible, especially because most of the immunization protocols have used whole recombinant MSPs [3]–[7] or membrane proteins fractions [14], [30] with significant protection against challenges, as shown by decreased bacteremia, and significant titers against polypeptides, but with variable efficacy.…”

Section: Discussionmentioning

confidence: 99%

“…Several immunization models have been successfully used to determine the usefulness of novel antigens and strategies for vaccination, and most of the immunization protocols against A. marginale include several recombinant major surface proteins (MSPs) and plasmids [3]–[7]. Although the efficacy of these experimental vaccines is variable between and within experiments, they demonstrate the feasibility of a subunit vaccine approach for the establishment of immunization protocols against this disease.…”

Section: Introductionmentioning

confidence: 99%

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Epitope-Based Vaccines with the Anaplasma marginale MSP1a Functional Motif Induce a Balanced Humoral and Cellular Immune Response in Mice

et al. 2013

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Bovine anaplasmosis is a hemoparasitic disease that causes considerable economic loss to the dairy and beef industries. Cattle immunized with the Anaplasma marginale MSP1 outer membrane protein complex presents a protective humoral immune response; however, its efficacy is variable. Immunodominant epitopes seem to be a key-limiting factor for the adaptive immunity. We have successfully demonstrated that critical motifs of the MSP1a functional epitope are essential for antibody recognition of infected animal sera, but its protective immunity is yet to be tested. We have evaluated two synthetic vaccine formulations against A. marginale, using epitope-based approach in mice. Mice infection with bovine anaplasmosis was demonstrated by qPCR analysis of erythrocytes after 15-day exposure. A proof-of-concept was obtained in this murine model, in which peptides conjugated to bovine serum albumin were used for immunization in three 15-day intervals by intraperitoneal injections before challenging with live bacteria. Blood samples were analyzed for the presence of specific IgG2a and IgG1 antibodies, as well as for the rickettsemia analysis. A panel containing the cytokines’ transcriptional profile for innate and adaptive immune responses was carried out through qPCR. Immunized BALB/c mice challenged with A. marginale presented stable body weight, reduced number of infected erythrocytes, and no mortality; and among control groups mortality rates ranged from 15% to 29%. Additionally, vaccines have significantly induced higher IgG2a than IgG1 response, followed by increased expression of pro-inflammatory cytokines. This is a successful demonstration of epitope-based vaccines, and protection against anaplasmosis may be associated with elicitation of effector functions of humoral and cellular immune responses in murine model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%