Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802)

Carlos, Jaqueline; Matta, Sérgio Luís Pinto da

doi:10.1590/s1516-89132009000600018

Cited by 4 publications

(4 citation statements)

References 19 publications

(21 reference statements)

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“…The testes of both African catfish and Nile tilapia, observed in this present study to have tunica albuginea of loose connective tissues and the spermatic duct containing sperm cells, surrounded by connective tissue are in agreement with the earlier studies of Awaad et al (2014) in Nile tilapia and the pattern observed in most teleosts (Carlos and Matta, 2009). The African catfish and Nile tilapia, share the basic structures with other fishes (Meisner et al, 2000), avian (Friedlander and Parrish, 1997) and mammalian species (Ahmed et al, 2013).…”

Section: Histomorphologysupporting

confidence: 92%

Comparative Morphological Studies on Testes and Seminal Vesicles of African Catfish (Clarias gariepinus) and Nile Tilapia (Tilapia nilotica)

Danmaigoro¹,

Hena²,

Ibrahim³

et al. 2020

J. Anim. Vet. Adv.

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Comparative morphological studies on the testes and seminal vesicles in African catfish and Nile Tilapia were conducted with the aim of comparing morphological and their architectural differences using anatomical, histological and biometric techniques. A total of the 50 fishes comprising of 25 African catfish and 25 Nile Tilapia fishes were captured and used, their live bodyweights and standard body lengths of the fish were measured using weighing balance in grams (g) and Tape rule in centimeter (cm), respectively. Thereafter, a midline incision was made on the ventral surface of the abdominal column. The positions of the organs were carefully examined in situ with representative samples of each of the testes and the seminal vesicle taken for routine histological evaluations with histomorphometry of the spermatozoa structural index assessment. The findings of this work showed that, the testicular structure and seminal vesicles of both African catfish (Clarias gariepinus) and Nile tilapia (Tilapia nilotica) are morphologically different in sizes, shapes and weight but have some similarities in their colors and locations. The testicular lengths of the African catfish were longer than that of the Nile tilapia (p<0.05). However, the spermatozoa structural index did not differ statistically even though there was higher is catfish when compared with tilapia with exception of the spermatozoa tail length. Therefore, apart from the contribution of the present study to the knowledge of comparative anatomy in these species of fish, it has equally put forward a baseline data for further comparative studies on the testicular and seminal vesicular morphology in these species.

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Section: Histomorphologysupporting

confidence: 92%

Comparative Morphological Studies on Testes and Seminal Vesicles of African Catfish (Clarias gariepinus) and Nile Tilapia (Tilapia nilotica)

Danmaigoro¹,

Hena²,

Ibrahim³

et al. 2020

J. Anim. Vet. Adv.

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“…The cells are located eccentrically with one to three nucleoli [118]. For example, Leydig cells are stored in cysts in Lithobates catesbeianus and bullfrogs have cells with a higher proportion of nuclei than cytoplasm [116]. The Sertoli cells in Physalaemus cuvieri are voluminous, elongated, and ovoid [119].…”

Section: Sertoli and Leydig Cellsmentioning

confidence: 99%

“…These interstitial cells are essential for spermatogenesis since they synthesize testosterone and other androgens [ 94 , 107 ]. The Leydig cells occupy approximately one-quarter of the interstitial tissue [ 116 ]. They are either detached or in groups near the blood vessels [ 117 ].…”

Section: Introductionmentioning

confidence: 99%

A review of the reproductive system in anuran amphibians

et al. 2023

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Reproductive biology is an important topic that is well explored in many vertebrates, but information about frogs’ reproductive mechanisms could be improved. Therefore, this review aims to provide organized and specific information on frog reproduction. First, we developed schemes that illustrate the general information regarding reproductive biological mechanisms in frogs in a specific way. Then, we described the physiological, histological, and morphological mechanisms of each organ of the reproductive system of male and female frogs. Finally, this manuscript may contribute to a broader understanding of anuran reproductive biology. Since, understanding frogs’ reproductive system permits one to make a comparison with reproduction with other anurans.

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“…For the Neotropics, little has been studied about the processes of spermatogenesis and spermiogenesis in this group of organisms (Carezzano et al, 2013). It has been evidenced that the majority of anurans present a testis with cystic cellular organization, although with differences in aspects such as pigmentation and thickness of the albuginea tunic (Carlos & Matta, 2009;Guillama et al, 2014). Dark pigmentation in the testes has been described in some species and is caused by cells called melanocytes that are randomly distributed in the albuginea tunic and the interstitium (Aoki et al, 1969;Oliveira et al, 2002;Guillama et al, 2014).…”

mentioning

confidence: 99%

Histology and structure of the testicles in three species of Atelopus frogs (Anura: Bufonidae) endemic to the Sierra Nevada de Santa Marta, Colombia

2021

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Introduction: Testicular histology constitutes one of the least explored aspects in frogs of the genus Atelopus. This taxonomic group shows an alarming population decline; therefore, its reproductive biology is one of the greatest topics of interest for its conservation. Objective: To describe the testicular morphology and the spermatogenetic lineage cells in adult males of Atelopus laetissimus, Atelopus nahumae, and Atelopus carrikeri in the Sierra Nevada de Santa Marta, Colombia. Methods: During June – July 2017 and 2018, sampling was conducted in the localities of San Lorenzo and Páramo Cebolletas, Sierra Nevada de Santa Marta (SNSM), to collect 15 adult males, 5 per species. Testes samples were fixed in Bouin to be processed by the standard paraffin-embedding technique. Histological sections (3 mμ) were stained with Hematoxylin-eosin and Mallory-Heidenhain-Azan-Gomori's. For the description and photographic register of the germ cells, the photonic microscopy technique was used with the differential interference contrast system. Results: The testes are oval organs, compact, light yellow color, and with little vascularization. Externally, they are surrounded by a thin albuginea tunic constituted by regular dense connective tissue. Inside this layer, they are composed of numerous seminiferous tubules of hexagonal contour, in which germ cell cysts are distinguished at different stages of spermatogenesis (spermatogonia I and II, spermatocyte I and II, and early and late spermatids) and spermiogenesis (spermatozoa in fascicles and free spermatozoa). Separating the seminiferous structures is the interstitial tissue in which Leydig cells and blood vessels stand out. Additionally, in the cranial part of the testis, the Bidder's organ was found, formed by two distinguishable regions, the cortex and the medulla. In the cortex, there are previtellogénic oocytes of different sizes surrounded by a monolayer of flat follicular cells. For its part, the medullary region is the connective tissue that nourishes the oocytes and is constituted by blood capillaries. Conclusions: The gonads of the three species analyzed present a cystic cellular organization similar to other anurans, where all stages of spermatogenesis and spermiogenesis were identified, possibly indicating a continuous reproductive activity. Likewise, the Bidder’s organ is reported for the first time in the three Atelopus species, which allows suggesting a possible sexual reversion in case of a population decrease of females as a reproductive strategy.

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Microscopic morphology and testis morphometry of captivity-bred Adult bullfrogs (Lithobates catesbeianus Shaw, 1802)

Abstract: ABSTRACT

Cited by 4 publications

References 19 publications

Comparative Morphological Studies on Testes and Seminal Vesicles of African Catfish (Clarias gariepinus) and Nile Tilapia (Tilapia nilotica)

Comparative Morphological Studies on Testes and Seminal Vesicles of African Catfish (Clarias gariepinus) and Nile Tilapia (Tilapia nilotica)

A review of the reproductive system in anuran amphibians

Histology and structure of the testicles in three species of Atelopus frogs (Anura: Bufonidae) endemic to the Sierra Nevada de Santa Marta, Colombia

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