Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils

Abstract

This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO 2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation

Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowishtm-Aqua Fog

“…This result is consistent with the finding of other researchers who found that bioaugmentation was not effective for diesel fuel (Demque, et al, 1997;Mariano, et al, 2009). It is important to note that for both diesel and motor oil-contaminated samples, there was a mean decrease of between 16.8 and 127 ppm TPH for each additional day elapsed between sampling and GC/MS analysis (p-value = 0.05).…”

“…Bioaugmentation with Staphylococcus hominis and Kocuria palustris failed to cause any improvement in the degradation of diesel fuel in soil from three gas stations (Mariano, et al, 2009). Bioaugmentation was ineffective and possibly deleterious to diesel biodegradation in sand (Demque, Biggar, & Heroux, 1997).…”

“…Soil can become contaminated from leaky underground storage tanks and industrial facilities involved in the production and distribution of petroleum products (Mariano, de Angelis, Pirollo, Contiero, & Bonotto, 2009). Offshore oil spills release crude oil onto beaches and into estuaries (Jernelov, 2010).…”

Enhancement of phenol degradation by soil bioaugmentation with Pseudomonas sp. JS150

“…2009). Sometimes unfavourable environmental circumstances, especially during the first days after inoculation, may be a reason that bioaugmentation does not enhance the degradative potential of contaminated soil (Mariano et al. 2009; Silva et al.…”

“…What is important, stress because of drastic changes in environmental conditions may lead to loss of microbial viability and even death of inoculated cells (Goldstein et al 1985;van Veen et al 1997;Liu et al 2009). Sometimes unfavourable environmental circumstances, especially during the first days after inoculation, may be a reason that bioaugmentation does not enhance the degradative potential of contaminated soil (Mariano et al 2009;Silva et al 2009;Ruberto et al 2010). A success of microbial survival, phenols removal and subsequently the efficiency of their degradation were strongly correlated with soil organic matter content.…”