Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Neto, Sérgio Luiz Moreira; Matheus, Dácio Roberto; Machado, Kátia Maria Gomes

doi:10.1590/s1516-89132009000500003

Cited by 23 publications

(4 citation statements)

References 26 publications

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“…Our study demonstrates the ability of fungi to modify the pH values of culture medium already after 24 hours of cultivation. Consistently with our results, Moreira Neto and co-workers described the ability of Lentinus crinitus and Psilocybe castanella to modify the pH value of the culture medium, which was found to be an important parameter for both the growth of the mentioned fungal strains and the enzymatic system involved in RBBR decolourization [ 38 ].…”

Section: Resultssupporting

confidence: 91%

Whole-cell fungal transformation of precursors into dyes

Polak

Jarosz-Wilkołazka

2010

Microb Cell Fact

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BackgroundChemical methods of producing dyes involve extreme temperatures and unsafe toxic compounds. Application of oxidizing enzymes obtained from fungal species, for example laccase, is an alternative to chemical synthesis of dyes. Laccase can be replaced by fungal biomass acting as a whole-cell biocatalyst with properties comparable to the isolated form of the enzyme. The application of the whole-cell system simplifies the transformation process and reduces the time required for its completion. In the present work, four fungal strains with a well-known ability to produce laccase were tested for oxidation of 17 phenolic and non-phenolic precursors into stable and non-toxic dyes.ResultsAn agar-plate screening test of the organic precursors was carried out using four fungal strains: Trametes versicolor, Fomes fomentarius, Abortiporus biennis, and Cerrena unicolor. Out of 17 precursors, nine were transformed into coloured substances in the presence of actively growing fungal mycelium. The immobilized fungal biomass catalyzed the transformation of 1 mM benzene and naphthalene derivatives in liquid cultures yielding stable and non-toxic products with good dyeing properties. The type of fungal strain had a large influence on the absorbance of the coloured products obtained after 48-hour transformation of the selected precursors, and the most effective was Fomes fomentarius (FF25). Whole-cell transformation of AHBS (3-amino-4-hydroxybenzenesulfonic acid) into a phenoxazinone dye was carried out in four different systems: in aqueous media comprising low amounts of carbon and nitrogen source, in buffer, and in distilled water.ConclusionsThis study demonstrated the ability of four fungal strains belonging to the ecological type of white rot fungi to transform precursors into dyes. This paper highlights the potential of fungal biomass for replacing isolated enzymes as a cheaper industrial-grade biocatalyst for the synthesis of dyes and other commercially important products. The use of immobilized fungal biomass limits free migration of cells and facilitates their reuse in a continuous system for precursor transformation.

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Section: Resultssupporting

confidence: 91%

Whole-cell fungal transformation of precursors into dyes

Polak

Jarosz-Wilkołazka

2010

Microb Cell Fact

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“…High salt ion concentrations can significantly affect the role of Ganoderma laccase in decolorizing RBBR [33]. pH was also found to be an important parameter for enzymatic RBBR decolorization systems from Lentinus crinitus and Psilocybecastanella [34].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Qin

Adil

et al. 2019

Molecules

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Many dyes and pigments are used in textile and printing industries, and their wastewater has been classed as a top source of pollution. Biodegradation of dyes by fungal laccase has great potential. In this work, the influence of reaction time, pH, temperature, dye concentration, metal ions, and mediators on laccase-catalyzed Remazol Brilliant Blue R dye (RBBR) decolorization were investigated in vitro using crude laccase from the white-rot fungus Ganoderma lucidum. The optimal decolorization percentage (50.3%) was achieved at 35 °C, pH 4.0, and 200 ppm RBBR in 30 min. The mediator effects from syringaldehyde, 1-hydroxybenzotriazole, and vanillin were compared, and 0.1 mM vanillin was found to obviously increase the decolorization percentage of RBBR to 98.7%. Laccase-mediated decolorization percentages significantly increased in the presence of 5 mM Na+ and Cu2+, and decolorization percentages reached 62.4% and 62.2%, respectively. Real-time fluorescence-quantitative PCR (RT-PCR) and protein mass spectrometry results showed that among the 15 laccase isoenzyme genes, Glac1 was the main laccase-contributing gene, contributing the most to the laccase enzyme activity and decolorization process. These results also indicate that under optimal conditions, G. lucidum laccases, especially Glac1, have a strong potential to remove RBBR from reactive dye effluent.

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“…We can conclude that both azo and anthraquinone dyes are toxic and resistant to biodegradation; however, individual strains of saprotrophic fungi differ from each other not only in their capacity to decolorize these dyes, but also in their sensitivity to those dyes and to the products of their degradation. Anthraquinone-based dyes, considered as a derivatives of p-benzoquinones, are, in consequence of their structures, very resistant to degradation [66,82]. In the case of azo dyes, for successful degradation is necessary to disrupt the azo bonds at first and further efficient degradation of aromatic rings depends on the characteristics of functional groups and on its interaction with the azo bonds [2,[83][84][85].…”

Section: Discussionmentioning

confidence: 99%

Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

Eichlerová

Baldrián

2020

JoF

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An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi. The enzyme production was recorded in all three ecophysiology groups, but to a different extent. All WR and LD fungi produced laccase, and the majority of them also produced MnP. The strains belonging to BR lacked decolorization capabilities. None of them produced MnP and the production of laccase was either very low or absent. The most efficient decolorization of both dyes and the highest laccase production was found among the members of the orders Polyporales and Agaricales. The strains with high MnP activity occurred across almost all fungal orders (Polyporales, Agaricales, Hymenochaetales, and Russulales). Synthetic dye decolorization by fungal strains was clearly related to their production of ligninolytic enzymes and both properties were determined by the interaction of their ecophysiology and taxonomy, with a more relevant role of ecophysiology. Our screening revealed 12 strains with high decolorization capacity (9 WR and 3 LD), which could be promising for further biotechnological utilization.

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Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Cited by 23 publications

References 26 publications

Whole-cell fungal transformation of precursors into dyes

Whole-cell fungal transformation of precursors into dyes

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

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