High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the citrus black spot

Stringari, Danyelle; Glienke, Chirlei; Christo, Daniel de; Maccheroni, Walter; Azevedo, João Lúcio de

doi:10.1590/s1516-89132009000500002

Cited by 24 publications

(12 citation statements)

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“…), P. citrichinaensis is a very weak pathogen on various citrus species, P. citribraziliensis and P. brazilianae are nonpathogenic endophytes in citrus and mango, respectively, and P. capitalensis is the broad host range endophyte commonly referred to as G. mangiferae (Glienke et al, 2011). In addition to phylogenetic analyses based on DNA sequences, grouping of pathogenic and nonpathogenic Phyllosticta isolates from citrus was obtained using AFLP (Baldassari et al, 2008) and RAPD markers (Stringari et al, 2009). The variation in these markers was greater among nonpathogenic isolates than among pathogenic isolates (Baayen et al, 2002;Baldassari et al, 2008;Glienke et al, 2011;Stringari et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Maridueña-Zavala¹,

Er²,

Goss³

et al. 2014

Trop. plant pathol.

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Citrus black spot is an emerging disease in Florida since 2010. The causal agent is Phyllosticta citricarpa (teleomorph Guignardia citricarpa), but non-pathogenic P. capitalensis (teleomorph often referred to as G. mangiferae) is often isolated from black spot lesions. Florida isolates of P. citricarpa and P. capitalensis from citrus have not been characterized in detail. In this study, Phyllosticta species isolated from Florida citrus were compared with worldwide isolates using multi-locus sequencing of four conserved loci (rDNA ITS, TEF1, ACT, and GPDH genes). Moreover, the diversity within the two Phyllosticta species was compared based on the same four loci. DNA sequences of P. citricarpa and P. capitalensis were clearly distinct, coinciding with other P. citricarpa and P. capitalensis sequences from different continents. The species showed different population structures in Florida. P. citricarpa isolates did not exhibit genetic variation and were similar to strains from other continents. In contrast, Florida P. capitalensis isolates were distributed over five sequence groups. This study did not point to the potential origin of P. citricarpa and P. capitalensis in Florida. More variable genetic markers and isolates from various continents would be required to track the possible movement of these Phyllosticta species.

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Section: Introductionmentioning

confidence: 99%

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Maridueña-Zavala¹,

Er²,

Goss³

et al. 2014

Trop. plant pathol.

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“…This consolidated taxonomic distinction supports the exclusion from quarantine measures of plant material harbouring P. capitalensis. To this purpose, several rapid PCR assays have been developed [12][13][14][15][16][17][18][19][20]. The applicative use of these assays has enabled to exclude the presence of the pathogen in New Zealand, unlike what was previously assumed [21], and has supported the hypothesis of the possible endophytic occurrence of P. citricarpa in asymptomatic Citrus spp., as pointed out by several investigations (Table 1).…”

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confidence: 99%

Endophytic Fungi of Citrus Plants

Nicoletti

2019

Agriculture

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Besides a diffuse research activity on drug discovery and biodiversity carried out in natural contexts, more recently, investigations concerning endophytic fungi have started considering their occurrence in crops based on the major role that these microorganisms have been recognized to play in plant protection and growth promotion. Fruit growing is particularly involved in this new wave, by reason that the pluriannual crop cycle likely implies a higher impact of these symbiotic interactions. Aspects concerning occurrence and effects of endophytic fungi associated with citrus species are revised in the present paper.Agriculture 2019, 9, 247 2 of 13 similar but fast-growing, and producing conidia embedded within a thicker mucoid sheath [4][5][6][7][8]. The latter, characterized as a different species (Phyllosticta capitalensis), are known to be ubiquitous as endophytes in woody plants, having been reported from at least 70 botanical families [6,9,10]. Guignardia endophyllicola, treated as a separate species in a work also emphasizing its widespread endophytic occurrence [11], is at present recognized as a synonym. Differences between the two sister species also concern their metagenetic cycle. In fact, it has been ascertained that P. citricarpa is heterothallic, while P. capitalensis is homothallic [8]. This consolidated taxonomic distinction supports the exclusion from quarantine measures of plant material harbouring P. capitalensis. To this purpose, several rapid PCR assays have been developed [12][13][14][15][16][17][18][19][20]. The applicative use of these assays has enabled to exclude the presence of the pathogen in New Zealand, unlike what was previously assumed [21], and has supported the hypothesis of the possible endophytic occurrence of P. citricarpa in asymptomatic Citrus spp., as pointed out by several investigations (Table 1). Moreover, the two species have been clearly differentiated on account of their enzymatic profiles, with a higher expression of amylases, endoglucanases, and pectinases in P. citricarpa, suggesting a likely involvement of these enzymes in the pathogenic aptitude of the CBS agent [22]. Differences in terms of pathogenesis-related proteins have been confirmed after the genome sequencing of the two species, disclosing a higher number of coding sequences in P. citricarpa (15,206 versus 14,797). Such a difference has been interpreted considering the presence of growth and developmental genes involved in the expression of pathogenicity [23].The issue of detection of contaminated material imported from areas where the pathogen is endemic has also prompted investigations concerning the assortment of Phyllosticta spp. able to colonize citrus plants in either symptomatic or latent courses. Several revisions have been published [17,24], and novel species characterized, which consistently enlarge the citrus-associated consortium within this widespread genus. Particularly, the pathogenic P. citriasiana from south-east Asia [25], P. citrichinaensis from China [26], P. citrimaxima from Thailand...

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“…According to the author, the technique could be applied on different strains and populations of a same species, being of low accuracy when dealing with big taxonomic groups. The RAPD may not be very useful when applied to determine very elaborated phylogenies, evolving big taxonomic groups, but its results are recognized when specific groups are studied (Duncan et al, 1993;Hamelin et al, 1993;Manulis et al, 1994;Laroche et al, 1995;Gomes et al, 2009;Stringari et al, 2009;Gonçalves et al, 2010). Ro et al (2007) obtained good results when they used RAPD aiming to differentiate the strains of Pleurotus eryngii.…”

Section: Resultsmentioning

confidence: 99%

Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains

Rolim

Cavalcante

Urben

et al. 2011

Braz. arch. biol. technol.

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High molecular diversity of the fungus Guignardia citricarpa and Guignardia mangiferae and new primers for the diagnosis of the citrus black spot

Abstract: RAPD markers were used to investigate the distribution of genetic variability among a group of

Cited by 24 publications

References 16 publications

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Genetic variation among Phyllosticta strains isolated from citrus in Florida that are pathogenic or nonpathogenic to citrus

Endophytic Fungi of Citrus Plants

Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains

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