The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli

Missailidis, Sotiris; Bernardo-Filho, Mário; Farias, Gustavo Cordeiro de; Gomes, Roberto Alcantara; Hall, William J.; Keynes, Milton

doi:10.1590/s1516-89132008000700025

Cited by 2 publications

(4 citation statements)

References 11 publications

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“…In recent studies, it was reported; the genotoxic and cytotoxic effects of SnCl 2 can be altered by herbal extract, chemicals and pharmaceutics on E. coli 5,8,[10][11][12][13] . One of these studies was performed by using cauliflower which is in the same family with broccoli.…”

Section: Resultsmentioning

confidence: 99%

“…Since stannous dichloride (SnCl 2 ) is the most common reducing agent in 99m Tc radiopharmaceuticals preparation, it has been shown that severe biological effects on central nervous system and oral mucosa in animal studies 1,4 . Also it has been reported that SnCl 2 had lethal effect on Escherichia coli (E. coli) with cytotoxic and genotoxic effects [5][6][7][8] . Genotoxic effect of SnCl 2 may be directly on deoxyribonucleic acid (DNA) or indirectly by generating free radicals 9 .…”

Section: Introductionmentioning

confidence: 99%

“…In recent studies, some authors have also described that genotoxic and cytotoxic effects of SnCl 2 can be altered by herbal extract, chemicals and pharmaceutics on E. coli 5,6,[8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

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Anti toxic effect of broccoli extract on stannous dichloride toxicity

et al. 2012

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PURPOSE: Since Technetium-99m ( 99m Tc) has favorable physical and chemical characteristics, it is widely used radioisotope in Nuclear Medicine. However, stannous dichloride (SnCl 2 ) has been widely used as a reducing agent in labeling procedure of pharmaceutical with radionuclide, it has been realized that SnCl 2 have genotoxic and cytotoxic effects on biological systems. In previous studies, it has been shown that some herbal extract can reduce genotoxic and cytotoxic effects of SnCl 2 . In the present study, it is aimed to evaluate the effect of the broccoli extract on the survival of E. coli ATCC 25922 strain against to toxic effects of SnCl 2 . METHODS:Broccoli was extracted with methanol extraction. HPLC and TLC analysis of broccoli extract were performed. Then antitoxicity and dose response assays were performed on bacterial strain. RESULTS:The broccoli extract had dose dependent protective effect against SnCl 2 toxic effect on E. coli. CONCLUSIONS:The consumption of broccoli may alter the stannous dichloride toxicity. Broccoli extract may use as a new protective strategies against the toxic effect of SnCl 2 on patients who were taken 99m Tc radiopharmaceuticals. Key words: Broccoli. Stannous dichloride. Technetium-99m. Escherichia coli. Toxicity. RESUMOOBJETIVO: Em face de suas características físico-químicas, o Tecnécio-99m ( 99m Tc) é um radiofármaco amplamente utilizado na Medicina Nuclear. Todavia, o dicloreto de estanho (SnCl 2 ) tem sido largamente aplicado como um agente redutor no procedimento farmacêutico de marcação com radionuclídeos. Constatou-se que o SnCl 2 apresenta efeitos genotóxicos e citotóxicos nos sistemas biológicos. Em estudos prévios, foi demonstrado que alguns extratos de ervas podem reduzir tais efeitos. O estudo atual objetivou avaliar os efeitos do extrato de brócolis na sobrevida da cepa E. coli ATCC 25922, exposta ao efeito tóxico do SnCl 2.MÉTODOS: O extrato de brócolis foi obtido mediante extração com metanol. Analises com HPLC e TLC foram efetuadas. Avaliou-se a antitoxicidade e realizou-se um ensaio dose-resposta para uma cepa de bactérias. RESULTADOS:O extrato de brócolis mostrou um efeito protetor dose dependente para os efeitos tóxicos do SnCl 2 sobre a E. coli. CONCLUSÕES:O consumo de brócolis pode alterar a toxicidade do dicloreto de estanho . O extrato de brócolis pode ser utilizado como uma nova estratégia para proteção de pacientes contra os efeitos tóxicos do SnCl 2, nos quais foi administrado o radiofármaco Tecnécio-99m.Descritores: Brassica. Compostos Estanho. Tecnécio. Escherichia coli. Toxicidade. Anti toxic effect of broccoli extract on stannous dichloride toxicity

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anti toxic effect of broccoli extract on stannous dichloride toxicity

et al. 2012

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“…Stannous chloride has been suggested to decrease the survival of bacterial cultures by free radical generation (Bernardo-Filho et al, 1994;Agostinho et al, 2008) Natural products could abolish the effects of SnCl 2 decreasing the free radical production or as scavengers of these species (Almeida et al, 2007). Naproxen could protect bacterial cultures from the lethal SnCl 2 effect, probably decreasing free radical production (Fig.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biological effects of the naproxen

et al. 2011

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This work aims to evaluate biological effects of naproxen through of the labeling of blood constituents with technetium-99m (99mTc), survival of bacterial cultures and electrophoresis profile of plasmid DNA. Blood samples from Wistar rats were incubated with naproxen or with saline (0.9% NaCl) as control, and radiolabeling of blood constituents was performed. Influence of naproxen on the E. coli AB1157 culture growth and survival in the presence or absence of SnCl 2 was used to assess cytotoxic effect. Electrophoretic profile in agarose gels of bacterial plasmids in presence or absence of SnCl 2 was used to evaluate antioxidant and genotoxic potential of naproxen. Results obtained suggest that naproxen could not interfere on the labeling of blood constituents with 99mTc, but it could present cytotoxic effects and protect E. coli cultures from the lethal effect of stannous chloride at low concentrations. Moreover, naproxen could present genotoxic effect in isolated plasmid DNA.

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The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli

Abstract: This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood

Cited by 2 publications

References 11 publications

Anti toxic effect of broccoli extract on stannous dichloride toxicity

Anti toxic effect of broccoli extract on stannous dichloride toxicity

Evaluation of biological effects of the naproxen

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