Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Minozzo, João Carlos; Moura, Juliana de; Almeida, Sérgio Monteiro de; Soccol, Vanete Thomaz

doi:10.1590/s1516-89132008000600007

Cited by 4 publications

(2 citation statements)

References 36 publications

(43 reference statements)

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“…The reaction was developed with O-phenylene-diamine dihydrochloride (OPD) (Sigma Aldrich, USA) and the absorbance was measured at 492 nm with ELISA reader (Multiskan Go, Thermo Fisher, Finland). A positive ELISA result was defined as two standard deviations above the mean OD value of negative sera ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Satyaprakash¹,

Khan²,

Chaudhari³

et al. 2023

IJPA

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Background: Porcine cysticercosis, caused by metacestodes of Taenia solium is an important neglected zoonosis. We evaluated the presence of anti-cysticercal antibodies and T. solium specific DNA in pig sera and blood samples respectively collected from Maharashtra, India. Methods: A total of three antigens (Scolex Antigen (SA), Membrane Body Antigen (MBA) and Excretory-Secretory Antigen (ESA)) were prepared from metacestodes of T. solium and employed in an in-house developed indirect-IgG ELISA for serological screening of 1000 porcine sera samples at Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra, India. The ELISA positive sera samples were subjected to EITB Assay for detection of immunodominant peptides. An effort has been made for molecular detection of porcine cysticercosis by PCR assay targeting large subunit rRNA gene of T. solium from blood samples of the corresponding ELISA-positive pigs. Results: The overall seroprevalence of porcine cysticercosis employing SA, MBA and ESA was 12.6%, 8.7% and 12.5% respectively. The lower and medium molecular weight peptides were the most frequently recognised in EITB assay. The numbers of bands recognised in EITB assay were observed to be proportionate with the corresponding ELISA O.D. values. An amplification product of 286 bp was observed in 22.98% (20/87), 30.35% (30/99) and 17.14% (12/70) of the sero-positive samples against SA, ESA and MBA respectively. Conclusion: EITB still remains the gold standard serodiagnosis test for cysticercosis. The inclusion of a greater number of positive samples and purification of antigens may improve the diagnostic efficacy of the tests.

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Section: Methodsmentioning

confidence: 99%

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Satyaprakash¹,

Khan²,

Chaudhari³

et al. 2023

IJPA

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“…Indirect ELISA (Minozzo et al, 2008): The tests and reference values of the positive and negative sera were optimized after checkerboard titration (Sigma, USA). All reagents were used as 100µl/well, tested sera were diluted with PBS as 1:100, and Horseradish peroxidase-conjugated anti-human &/or anti-pig IgG was diluted as 1:2000.…”

Section: Introductionmentioning

confidence: 99%

Antigens From Pig Cysticercosis and Their Valuability in Diagnosis of Human Cysticercosis

Abdel-Radi

El-Hafez

Morsy

2020

Journal of the Egyptian Society of Parasitology

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Evaluation of the protective potential of a Taenia solium cysticercus mimotope on murine cysticercosis

Capelli-Peixoto

Chávez-Olórtegui

Chaves-Moreira

et al. 2011

Vaccine

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An NC-1 mimotope from Taenia solium cysticerci can help identify patients with neurocysticercosis through immunoassay. After chemical synthesis, an NC-1 peptide was coupled to bovine serum albumin (NC-1/BSA) for used as an immunogen in murine Taenia crassiceps cysticercosis, which is an experimental model of cysticercosis caused by T. solium. NC-1/BSA immunisation decreased parasitaemia by inducing 74% protection compared to the 77% protection obtained with T. crassiceps crude antigen. The influence of immunisation was also observed on the size and stage of development of the parasite. Antibodies from NC-1/BSA-immunised mice recognised proteins from the tegument and from the buddings, and intense immunostaining was observed in the final stage of the metacestode. The capacity of NC-1/BSA to induce protective antibodies which are reactive to proteins from the tegument of the metacestode suggests that this mimotope is a potential candidate for a vaccine against human and animal cysticercosis.

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Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Abstract: Neurocysticercosis (NCC), the cerebral presence of

Cited by 4 publications

References 36 publications

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Antigens From Pig Cysticercosis and Their Valuability in Diagnosis of Human Cysticercosis

Evaluation of the protective potential of a Taenia solium cysticercus mimotope on murine cysticercosis

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