An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum

Abstract

The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump.

A procedure for correcting the position of plant tissue samples in accidentally polymerized glycol methacrylate resin

“…Although the obtainment of sections made in paraffin is faster than that of ones in resin, the infiltration and embedding procedures are much slower and more difficult, requiring the use of xylol (Johansen 1940, Berlyn & Miksche 1976, a solvent which is highly prejudicial to the sample cell (Amaral et al 2004) and, apparently, carcinogenic (Kraus & Arduin 1997). Very often one must submit the sample to hot vacuum in order to obtain satisfactory results (Leitão & Cortelazzo 2008).…”

Working Optimally with Serial Sections in Glycol Methacrylate Resin

“…Although the distribution of serial sections in historesin slide set has already been discussed, this principle also applies to sections of paraffin embedded material, provided that it can properly accommodate at least two rectilinear ribbons per slide. The elaboration of the present work was another initiative aimed at working with science in an optimized, economic, accessible and ecologically conscious way [6][7][8][9][10][11][12][13] .…”