Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed

Das, Mithu; Banerjee, Rintu; Bal, Satish

doi:10.1590/s1516-89132008000100005

Cited by 29 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21 The activity of laccase was determined by spectrophotometrically. 22 One units of laccase activity was dened as the amount of enzyme required to oxidize 1 mmol of ABTS per minute under the assay conditions whereas, cellulase activity was determined by following assay protocols.…”

Section: Enzymementioning

confidence: 99%

Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production

Rajak

Banerjee

2016

RSC Adv.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Enzymementioning

confidence: 99%

Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production

Rajak

Banerjee

2016

RSC Adv.

Self Cite

View full text Add to dashboard Cite

show abstract

“…An increase in initial pH of the medium beyond 4.5 reduced the cellulase production drastically. Yang et al [20] and Das et al [21] also observed the optimum cellulase production at pH 4.5, respectively, when studying the cellulase production using Trichoderma sp.…”

Section: Effect Of Initial Phmentioning

confidence: 92%

“…Any further increase in temperature decreased the enzyme production, which may be attributed to lower fungal growth at higher temperature. Yang et al [20] and Das et al [21]. also reported an optimum temperature ranging 28-30°C for cellulase production from Trichoderma sp.…”

Section: Effect Of Different Physiological Parameters On Cellulase Prmentioning

confidence: 98%

Cost-effective production of cellulose hydrolysing enzymes from Trichoderma sp. RCK65 under SSF and its evaluation in saccharification of cellulosic substrates

Chakraborty

Gupta

Jain

et al. 2016

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Trichoderma sp. is a potential cellulase producing mesophilic fungi which grow under mild acidic condition. In this study, growth and nutritional conditions were manipulated for the maximum and cost-effective production of cellulase using lab strain Trichoderma sp. RCK65 and checked for its efficiency in hydrolysis of Prosopis juliflora (a woody substrate). Preliminary studies suggested that when 48 h old secondary fungal culture (20 % v/w) was inoculated in wheat bran moistened with mineral salt solution (pH 4.5 and 1:3 solid to moisture ratio), incubated at 30°C and after 72 h, it produced maximum cellulase (CMCase 145 U/gds, FPase 38 U/gds and b-glucosidase 105 U/gds). However, using statistical approach a S:L ratio (1:1) was surprisingly found to be optimum that improved cellulase that is CMCase activity by 6.21 %, FPase activity by 23.68 % and b-glucosidase activity by 37.28 %. The estimated cost of crude enzyme (Rs. 5.311/1000 FPase units) seems to be economically feasible which may be due to high enzyme titre, less cultivation time and low media cost. Moreover, when the crude enzyme was used to saccharify pretreated Prosopis juliflora (a woody substrate), it resulted up to 83 % (w/w) saccharification.

show abstract

“…This result is supported by many researchers who found that the cellulase production by Trichoderma spp. is favored by acidic pH [19][20] . Table 1 show that cellulase production by T. viride was significantly influenced by the type of carbon source.…”

Section: Effect Of Ph On Enzyme Production By T Viridementioning

confidence: 99%

Strain Improvement of Trichoderma Viride through Mutation for Enhanced Production of Cellulase

Khandoker

Mamun

Nafiz

et al. 2016

Bangla. J. Microbiol.

View full text Add to dashboard Cite

Microbial fungal cellulases are very important for their applications in biopolishing of textile fibres, in poultry feed and paper and pulp industries. The main purpose of this study was to improve the wild strain Trichoderma viride for enhanced production of cellulase by random mutation technique employing ultraviolet (UV) irradiation and Ethidium bromide (EtBr) treatments. The wild strain exhibited the highest cellulase activity 5.52 U/ml on carboxy methyl cellulose (1.0%) and a comparable activity 4.74 U/ml on wheat bran (1.0%) as substrate under the optimum temperature 30°C and pH 4.0. Upon mutation by UV exposure the fungi produced cellulase 11.28 U/ml where as EtBr treated mutant showed 14.61 U/ml cellulase activities. Both the enzymes from wild and mutant T. viride demonstrated the highest activity at the assay temperature of 40ºC. The enzyme was applied for bio-polishing of jeans at prototype experiment and showed effective result as compared to one of the commercial enzymes.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 43-47

show abstract

Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed

Abstract: ABSTRACT

Cited by 29 publications

References 16 publications

Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production

Enzyme mediated biomass pretreatment and hydrolysis: a biotechnological venture towards bioethanol production

Cost-effective production of cellulose hydrolysing enzymes from Trichoderma sp. RCK65 under SSF and its evaluation in saccharification of cellulosic substrates

Strain Improvement of Trichoderma Viride through Mutation for Enhanced Production of Cellulase

Contact Info

Product

Resources

About