Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Barreto, George E.; Oliveira, Elineusa Silva de; Villar, Flávio Augusto Guerreiro Aragão de; Santos, Gleide Souza dos; Lima, Rute Maria Ferreira; Costa, Sílvia Lima; Costa, Maria de Fátima Dias; Egito, Eryvaldo Sócrates Tabosa do; El-Bachá, Ramon dos Santos

doi:10.1590/s1516-89132007000500012

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Due to the intensive utilization of BTEX in several human activities (Hutchins et al, 1991;Barreto et al, 2007), this compound is commonly found in the environment and mainly associated to groundwater contamination (Lovley, 1997).…”

Section: Discussionmentioning

confidence: 99%

Cellular damages in the Allium cepa test system, caused by BTEX mixture prior and after biodegradation process

2011

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Petroleum and derivatives have been considered one of the main environmental contaminants. Among petroleum derivatives, the volatile organic compounds benzene, toluene, ethylbenzene and xylene (BTEX) represent a major concern due to their toxicity and easy accumulation in groundwater. Biodegradation methods seem to be suitable tools for the clean-up of BTEX contaminants from groundwater. Genotoxic and mutagenic potential of BTEX prior and after biodegradation process was evaluated through analyses of chromosomal aberrations and MN test in meristematic and F(1) root cells using the Allium cepa test system. Seeds of A. cepa were germinated into five concentrations of BTEX, non-biodegraded and biodegraded, in ultra-pure water (negative control), in MMS 4×10(-4)M (positive control) and in culture medium used in the biodegradation (blank biodegradation control). Results showed a significant frequency of both chromosomal and nuclear aberrations. The micronucleus (MN) frequency in meristematic cells was significant for most of tested samples. However, MN was not present in significant levels in the F(1) cells, suggesting that there was no permanent damage for the meristematic cell. The BTEX effects were significantly reduced in the biodegraded samples when compared to the respective non-biodegraded concentrations. Therefore, in this study, the biodegradation process showed to be a reliable and effective alternative to treat BTEX-contaminated waters. Based on our results and available data, the BTEX toxicity could also be related to a synergistic effect of its compounds.

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Section: Discussionmentioning

confidence: 99%

Cellular damages in the Allium cepa test system, caused by BTEX mixture prior and after biodegradation process

2011

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“…25 Furthermore, 3-methylcatechol also inhibited state 2 FADH 2 -linked respiration in rat brain nonsynaptic mitochondria. 26 Cells can die by two major mechanisms: necrosis or apoptosis. One apoptotic pathway involves the participation of mitochondria through the induction of leakiness of the external mitochondrial membrane, leading to the release of cytochrome c into the cytosol.…”

Section: Discussionmentioning

confidence: 99%

Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis

et al. 2010

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The exposure to benzene is a public health problem. Although the most well-known effect of benzene is hematopoietic toxicity, there is little information about the benzene and its metabolites effects on the central nervous system (CNS). This study examined the toxic effects of 1,2-dihydroxybenzene (catechol), a benzene metabolite, to human glioblastoma GL-15 cells. GL-15 cell cultures were used as a model to provide more information about the toxic effects of aromatic compounds to the CNS. Catechol induced time- and concentration-dependent cytotoxic effects. Morphological changes, such as the retraction of the cytoplasm and chromatin clumping, were seen in cells exposed to 200 μM catechol for 48 hours. In cells exposed to 600 μM catechol for 48 hours, 78.0% of them presented condensed nuclei, and the Comet assay showed DNA damage. The percentage of cells labeled with annexin V (apoptotic cells) was greater in the group exposed to catechol (20.7%) than in control cells (0.4%). Exposure to catechol at concentrations greater than 100 μM enhanced Bax levels, and a decrease in Bcl-2 level was observed after the exposure to 600 μM catechol for 48 hours. Furthermore, catechol depleted reduced glutathione. Hence, catechol induced cell death mainly by apoptosis.

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“…BTEX are commonly used by the chemical industry to prepare raw materials to manufacture pesticides, plastic, synthetic fibers, solvents, paints, foundries, electronics and pharmaceuticals [10]. These compounds tend to accumulate in the indoor occupational environment, exposing the workers to their risks [2], [11], [12].…”

Section: Introductionmentioning

confidence: 99%

Btex in an occupational environment

Martins¹,

Borba²,

Santos³

et al. 2019

Int. J. EI

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The BTEX group (benzene, toluene, ethyl benzene and xylene) are known for their potential toxic, mutagenic and carcinogenic effects, especially in an indoor occupational environment, where these substances dissipate with greater difficulty. Thus, the present work evaluated the concentrations, mutagenicity and cytotoxicity of the BTEX group in the indoor air of workshops involving painting and varnishing. Samples were collected using air pumps from the outside of the open environment workshops and from the inside of each of the three workshops. The chemical analyses were carried out using gas chromatograph with mass spectrometry. The mutagenic and cytotoxicity potentials were determined using the Salmonella/microsome and WST/LDH assays, respectively. The concentrations of each constituent of the BTEX group were below the limits established by the National Institute for Occupational Safety and Health and toluene presented the highest value. Moreover, these compounds did not induce mutagenic activity in the TA98 and TA100 Salmonella typhimurium strains either in the presence or in the absence of metabolization, and no cytotoxic effects were observed in the A549 human lung cells. These results may be related to the low BTEX values found in the occupational environment, as can be seen in some other studies. Nevertheless, at low concentrations, these compounds may cause toxicity by a pathway not investigated in this study or may have interacted with other non-monitored air constituents, reducing their toxicity. The present study sought to obtain more information and clarifications regarding occupational exposure to BTEX, contributing to the risk assessment of the workers exposed to these substances.

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Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Abstract: ABSTRACT

Cited by 8 publications

References 25 publications

Cellular damages in the Allium cepa test system, caused by BTEX mixture prior and after biodegradation process

Cellular damages in the Allium cepa test system, caused by BTEX mixture prior and after biodegradation process

Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis

Btex in an occupational environment

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