Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

Vieira, Felipe; Cunha, Maura Da; Klein, Denise Espellet; Carvalho, André de Oliveira; Gomes, Valdirene Moreira

doi:10.1590/s1516-89132006000700004

Cited by 8 publications

(1 citation statement)

References 35 publications

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“…Proteins have also been reported as a component of the secretion produced by colleters from a variety of different species (Thomas & Dave 1990;Klein et al 2004;Miguel et al 2006;González & Tarragó 2009;Mayer et al 2011;Dalvi et al 2013). Some authors have suggested that such proteins may provide additional protection from fungi and parasites (Miguel et al 2006;Vieira et al 2006;Mayer et al 2011). However, research aiming to unravel how exudates produced by colleters may contribute to the success of species of Chamaecrista in stressful environments is still needed.…”

Section: Discussionmentioning

confidence: 99%

Colleters in Chamaecrista (L.) Moench sect. Chamaecrista and sect. Caliciopsis (Leguminosae-Caesalpinioideae): anatomy and taxonomic implications

et al. 2017

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Th e genus Chamaecrista contains ca. 330 species organized into six sections, of which section Chamaecrista is the second largest (ca.75 species) distributed among six series, while the section Caliciopsis contains only two species. Colleters have been described in the genus Chamaecrista and they show potential taxonomic and phylogenetic signifi cance. Th ey are associated with lubrication, desiccation prevention and protection from microbial attacks of young developing organs. Although six types of colleters have been described for the genus Chamaecrista, there have been no studies focusing on the diversity of colleters in the sections Chamaecrista and Caliciopsis. Samples from developing leaves and fl owers of both sections were obtained from herbarium and fi eld collections and subjected to standard methodologies for both light and scanning electron microscopy. Histochemical tests were also performed to determine the nature of the exudates. Five types of non-vascularized colleters were found: short digitiform, long digitiform, club-shaped, pyriform and short bottle-shaped. Polysaccharides, pectins, lipids and proteins were detected in the exudates of all types of colleters. Among the fi ve types of colleters observed, pyriform is a novelty for Chamaecrista, reinforcing the signifi cant morphological diversity of these secretory structures in this genus.

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Section: Discussionmentioning

confidence: 99%

Colleters in Chamaecrista (L.) Moench sect. Chamaecrista and sect. Caliciopsis (Leguminosae-Caesalpinioideae): anatomy and taxonomic implications

et al. 2017

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Purification and Partial Characterization of a Novel β-1,3-Endoglucanase from Streptomyces rutgersensis

et al. 2013

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A β-1,3-endoglucanase produced by Streptomyces rutgersensis was purified to a homogeneity by the fractional precipitation with ammonium sulfate, ion exchange chromatography on Q-Sepharose and hydrophobic chromatography on Butyl Sepharose. A typical procedure provided 11.74-fold purification with 12.53 % yield. SDSPAGE of the purified protein showed one protein band. The exact molecular mass of the enzyme obtained by mass spectrometry was 41.25 kDa; the isoelectric point was between pH 4.2–4.4. The optimal β-glucanase catalytic activity was at pH 7 and 50 °C. An enzyme was only active toward glucose polymers containing β-1,3 linkages and hydrolyzed Saccharomyces cerevisiae cell wall β-glucan in an endo-like way: reaction products were different molecular size β-glucans, which were larger than glucose.

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Secretory structures at syconia and flowers of Ficus enormis (Moraceae): A specialization at ostiolar bracts and the first report of inflorescence colleters

Machado

Souza

Leitão

2013

Flora - Morphology, Distribution, Functional Ecology of Plants

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Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

Cited by 8 publications

References 35 publications

Colleters in Chamaecrista (L.) Moench sect. Chamaecrista and sect. Caliciopsis (Leguminosae-Caesalpinioideae): anatomy and taxonomic implications

Colleters in Chamaecrista (L.) Moench sect. Chamaecrista and sect. Caliciopsis (Leguminosae-Caesalpinioideae): anatomy and taxonomic implications

Purification and Partial Characterization of a Novel β-1,3-Endoglucanase from Streptomyces rutgersensis

Secretory structures at syconia and flowers of Ficus enormis (Moraceae): A specialization at ostiolar bracts and the first report of inflorescence colleters

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