Production of specific polyclonal antibodies anti-human factor IX

Chaves, Daniel Gonçalves; Rodrigues, Cibele V.; Ferreira, Wanderley Almeida; Siqueira, Pollyana Fantini Miranda; Guerra-Duarte, Clara; Carneiro-Proietti, Anna Bárbara de Freitas; Santoro, Marcelo M.

doi:10.1590/s1516-89132006000500010

Cited by 2 publications

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References 9 publications

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“…Using a bacterially derived hFVIII-C1C2 peptide and plasma samples from hemophilia A inhibitor patients in a previous work (unpublished data), we demonstrated a specific Ab/Ag reaction between the bacterially derived hFVIII subfragment and the patient's plasma that supports the idea of probable medical application of bacterially produced peptides as neutralizing factor in FVIII inhibitor-generating patients [38]. Production of specific polyclonal antibodies antihuman factor IX using the whole hFIX protein was reported previously by Chaves and colleagues [39]. The application of that polyclonal antibody against the whole hFIX protein might be limited to experiments dealing with the entire length of hFIX protein.…”

Section: Discussionsupporting

confidence: 84%

Use of a Bacterially Expressed Human Factor IX Light Chain to Develop Polyclonal Antibody Anti-hFIX

Safari

Zomorodipour

Amirmozaffari

et al. 2009

Appl Biochem Biotechnol

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Hemophilia B is an X-linked recessive bleeding disorder caused by deficiency or malfunctioning of human coagulation factor IX (hFIX). Hemophilia B patients are treated at present by infusion of plasma derived hFIX which is not always efficient, because development of anti-hFIX antibodies (alloantibodies) in some cases inhibits the activity of the infused hFIX. The hFIX alloantibodies are directed against gamma-carboxyglutamic acid residues (Gla-domain) or protease domain in hFIX light chain. An epitope-containing fragment of hFIX light-chain was expressed in a T7-based Escherichia coli expression system and after purification, it was used for the immunization of rabbit to develop specific antibodies anti-hFIX. The plasma, derived from the immunized rabbit, was shown to be able to detect the normal hFIX, which indicates for the presence of a specific anti-hFIX antibody and supporting that a bacterially expressed hFIX subfragment might be able to neutralize the alloantibodies. Considering the importance of hFIX and its related investigations, both the produced hFIX antigen and its corresponding antibody will play important roles for experiments dealing with the production of hFIX and studies involved in the neutralization of the hFIX inhibitors in hFIX-related disorders and other clinical applications.

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Section: Discussionsupporting

confidence: 84%

Use of a Bacterially Expressed Human Factor IX Light Chain to Develop Polyclonal Antibody Anti-hFIX

Safari

Zomorodipour

Amirmozaffari

et al. 2009

Appl Biochem Biotechnol

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“…Os ensaios foram realizados a 15 0 C, 25 0 C e 37 0 C e os resultados são apresentados na Figura 55. Ao analisar a correlação entre a concentração de IgG h e a variação da agregação das nanopartículas (A 396 /A 564 ) observa-se uma sensibilidade maior ao utilizar a temperatura de 15 0 C. Este resultado é coerente com aqueles apresentados por outros grupos de pesquisa (Thanh;Rosenzweig, 2002;Yuan et al, 2011;Yuan et al, 2012) Por meio do imunoensaio de anti-agregação é possível detectar IgG h em concentrações próximas ou mesmo inferiores à detecção proporcionada por alguns métodos Elisa e radioimunoensaios (Woo et al, 1979;Kan, Verspaget;Peña, 1983 (Delfos, 1991;Stridsberg et al, 2003;Vinik et al, 2009) e o fator IX de coagulação (Chaves et al, 2006;Ørstavik;Ørstavik, 1981…”

Section: Imunoensaio De Anti-agregação Para Determinação Deunclassified

Preparação e caracterização de nanopartículas de prata para aplicação no desenvolvimento de imunoensaio para imunoglobulina G humana

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Ao professor Renato, pela orientação, confiança, incetivo e compreensão. A todos os professores do laboratório de Química de Pesquisa em Química Ambiental. Em especial, ao professor Cassius, por todo apoio e sugestões. A todos os colegas do grupo de Química de Pesquisa em Química Verde e Ambiental. Em especial agradeço à Raquel e à Andressa, que sempre me ajudaram durante a pós-gradução. A professora Elisabeth Cheng e os membros do laboratório de bioprocessos do Instituto Butantan, que me auxiliaram em vários momentos durante o desenvolvimento do meu projeto. A todos os professores e funcionários do Instituto de Química que também colaboraram com o desenvolvimento do projeto. Em especial agradeço ao Alfredo, funcionário da Central Analítica do IQ-USP, por toda a ajuda prestada. Ao CNPq e a Fapesp pelo apoio financeiro. Por fim, agradeço a minha família, em especial, meus pais, Antonio Carlos e Marli, por todo apoio e incentivo. Palavras-chave: imunoensaio; imunoglobulina G humana; nanopartículas de prata.

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Production of specific polyclonal antibodies anti-human factor IX

Abstract: In this work, polyclonal antibodies anti-human

Cited by 2 publications

References 9 publications

Use of a Bacterially Expressed Human Factor IX Light Chain to Develop Polyclonal Antibody Anti-hFIX

Use of a Bacterially Expressed Human Factor IX Light Chain to Develop Polyclonal Antibody Anti-hFIX

Preparação e caracterização de nanopartículas de prata para aplicação no desenvolvimento de imunoensaio para imunoglobulina G humana

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