Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss

Ninhaus‐Silveira, Alexandre; Foresti, Fausto; Tabata, Yara Aiko; Rigolino, Marcos Guilherme; Veríssimo‐Silveira, Rosicleire

doi:10.1590/s1516-89132006000100009

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…For this reason, a high rate of thawing is usually used in order to diminish the level of recrystallization. When the thawing is rapid, there is not enough time for the dehydrated cells to absorb the quantity of water the lost during freezing (Ninhaus-Silveira et al, 2006a;NinhausSilveira et al, 2006b). In March / 2004, it was reported that a baby was born from semen frozen for 21 years and sent to the in vitro fertilization technique (Horne et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Spermatic feasibility after 20 years of freezing and refreezing: case study

Taitson

Souza

2010

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

Spermatic feasibility after 20 years of freezing and refreezing: case study

Taitson

Souza

2010

Braz. arch. biol. technol.

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“…It also needs to be considered that steroids concentrations may decrease with storage time, especially at room temperature [ 21 , 40 ]. Optimising the dose and duration of treatment with masculinising agents is of the utmost importance; too-low concentrations may be ineffective (however, it may be useful for the production of functional males [ 38 ]), whereas too high doses often have a so called “paradoxical feminisation” or a sterilising effect [ 1 , 41 , 42 ].…”

Section: Hormonal Induction Of Sex Reversalmentioning

confidence: 99%

“…The first attempt was conducted by [ 12 ] who cryopreserved the semen of SRF Chinook salmon in pellets using 0.3 M glucose and 10% DMSO and obtained fertilisation rates of about 80%. The use of an extender consisting of glucose, DMSO, and egg yolk led to the fertilising ability of the cryopreserved sperm of SRF rainbow trout being about 60% [ 24 , 38 ]. The cryopreservation of SRF rainbow trout semen can be also performed using multicomponent mineral solution of [ 123 ] supplemented with only DMSO [ 106 ] or DMSO, egg yolk, and Dan Pro S760 [ 102 ].…”

Section: Cryopreservation Of Srf Spermmentioning

confidence: 99%

“…The most important parameter for the evaluation of sperm quality in cryopreserved semen is the sperm fertilising ability. Similar to motility, the fertilising ability of cryopreserved SRF sperm is mostly lower than the values obtained using fresh semen [ 38 , 106 ]. In contrast, the cryopreservation of SRF rainbow trout semen using a GM extender resulted in a high fertilising ability (80–90%), similar to values obtained using fresh semen [ 61 , 64 , 66 ].…”

Section: Cryopreservation Of Srf Spermmentioning

confidence: 99%

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Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Judycka

Nynca

Hliwa

et al. 2021

IJMS

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Sex reversal has been used as a breeding strategy by salmonid fish to produce genetically and phenotypically single sex populations. Production of all-female fish has great importance for the creation of monosex female triploids of salmonid fish, which are valued for their sterility, lack of female maturation, and larger commercial size. Among salmonids, the majority of rainbow trout (Oncorhynchus mykiss) production is based on all-female production with a high proportion of all-female triploid production in Europe. The main aim of this review is to present the recent knowledge regarding sex-reversed females (SRFs) of salmonid fish. We discuss the methods of sex reversal as well as their effects on the morphology and histology of the reproductive tract. We focus on the characteristics of SRF semen as well as the factors determining semen quality. The lower quality of SRF sperm compared to that of normal males has resulted in the need for the artificial maturation of semen. Most importantly, methods of semen storage—both short-term and long-term (cryopreservation)—that can improve hatchery operations are presented with the special emphasis on recent progress in development of efficient cryopreservation procedures and use of cryopreserved semen in hatchery practice. Moreover, we also address the emerging knowledge concerning the proteomic investigations of salmonid sperm, focusing primarily on the proteomic comparison of normal male and SRF testicular semen and presenting changes in SRF rainbow trout sperm proteome after in vitro incubation in artificial seminal plasma.

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“…(), L = Kirankumar and Pandian (), M = Ninhaus‐Silveira et al . (), N = Balasubramani and Pandian () and O = Thresher et al . ().…”

Section: Review Of Sperm Trait Effect Sizes and Meta‐analyses In Gonomentioning

confidence: 99%

Sperm traits of masculinized fish relative to wild‐type males: a systematic review and meta‐analyses

2014

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Environmental sex reversal (ESR), whereby environmental effects (e.g. exogenous chemicals) override genetic sex determination, is a commonly used technique in aquaculture and physiology research. We performed a systematic review and meta‐analyses of the literature that compares the sperm characteristics of masculinized genotypic females to wild‐type males. We detected no mean differences between the ejaculate volume, sperm motility, duration or linearity of each type of male. We found some large mean differences in sperm concentration (d = 2.541, CI = −0.004 to 5.086), reproductive success (d = −1.400, CI = −2.943 to 0.142), semen osmolality (d = 1.850, CI = 0.622 to 3.077) and sperm velocity (d = −0.933, CI=−1.426 to −0.441); in the case of the latter two traits, the mean effect was statistically significant. However, any significance did not stand up to a more conservative analysis. Additionally, heterogeneity was high and we found that where large differences between the sperm of sex‐reversed and wild‐type males are reported, these effects are attributable to sperm sampling methodology. Overall, we found little evidence for large systematic differences between the sperm produced by masculinized and wild‐type male fish. Thus, masculinized genotypic females may enjoy reproductive success comparable to genotypic males. This conclusion leads to two potential implications: (i) sex‐reversed fish may influence the dynamics of wild populations and (ii) aquaculture practices may use ESR to produce males with sperm quality similar to that of genotypic males. Most studies appear to have been performed in aquaculture species (i.e. Salmonidae); thus future experiments in non‐model organisms may provide important insights in to the uniformity of the effects described.

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Cryopreservation of semen from functional sex-reversed genotypic females of the rainbow trout, Oncorhynchus mykiss

Cited by 10 publications

References 12 publications

Spermatic feasibility after 20 years of freezing and refreezing: case study

Spermatic feasibility after 20 years of freezing and refreezing: case study

Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Sperm traits of masculinized fish relative to wild‐type males: a systematic review and meta‐analyses

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