Morphogenetic response of cotyledon and leaf explants of melon (Cucumis melo L.) cv. Amarillo Oro

Abstract

Callus cultures from cotyledon and leaf explants of a Spanish cultivar of melon (Amarillo

Cefotaxime and Benzyladenine Improve Melon Regeneration

“…Additionally, different plant genotype and varieties exhibit quite different regeneration rates (18). Explant age and gelling agents may also affect the melon regeneration efficiency (19).…”

Agrobacterium-mediated transformation and shoot regeneration in elite breeding lines of western shipper cantaloupe and honeydew melons (Cucumis melo L.)

“…Six regeneration media, RM1 (Ficcadenti and Rotino 1995), RM2 (Guis et al 2000), RM3 (Yadav et al 1996), RM4 (Fang and Grumet 1990), RM5 (Souza et al 2006), and RM6 (Bordas et al 1997), were evaluated with our melon genotypes (Table 1). Each medium represented the optimal composition to give the highest regeneration frequency in each study reported.…”

“…The synthetic auxin 2,4-dichlorophenoxyacetic acid has been widely used to induce somatic embryogenesis (AkasakaKennedy et al 2004;Gray et al 1993;Guis et al 1997;Kintzios et al 2004;Kintzios and Taravira 1997;Oridate and Oosawa 1986). In addition, 6-(c,c-dimethylallylamino)-purine (2iP), gibberellic acid (GA 3 ), kinetin, thidiazuron and a-naphthaleneacetic acid have been used in melon culture (Fang and Grumet 1990;Ficcadenti and Rotino 1995;Guis et al 2000;Souza et al 2006;Yadav et al 1996). The effects of these growth regulators on melon regeneration differed depending on plant genotype, explant type and culture conditions.…”

The Effects of Different Combinations and Varying Concentrations of Growth Regulators on the Regeneration of Selected Turkish Cultivars of Melon

“…To date propagation of Cucumis melo has been reported by different research groups [34,36,48,49,50,51]. The main differences between the present study and the earlier ones are the growth regulators used and melon cultivars selected.…”

“…The main differences between the present study and the earlier ones are the growth regulators used and melon cultivars selected. We used Hasanbey and Cinikiz melon cultivars as explants; while the others used 'Amarillo oro' [48,51], 'Accent', 'Galia', 'Presto', and 'Viva' [36], 'Revigal' and 'Kirkagac' [34,50], 'Topmark' [49,50]. After three weeks on a MS medium free from plant growth regulator, approximately 80% of cotyledon explants gave rise to friable callus ( Figure 5).…”