Advances in bacterial specific imaging

Wareham, David W.; Michael, J.; Das, Sreena

doi:10.1590/s1516-89132005000700021

Cited by 28 publications

(18 citation statements)

References 15 publications

(12 reference statements)

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“…1,8 In this context, radiolabeled antibiotics that can be incorporated and metabolized by the bacteria present in the infectious foci, allowing for an accurate and specific identification of infectious sites, are of great interest. [8][9][10][11] Ceftizoxime (CFT) ( Figure 1A) is a broad-spectrum antibiotic that binds to the bacterial cell wall and inhibits the formation of peptidoglycan, impairing the formation of the cell wall, and in turn killing the bacteria. Scintigraphic imaging studies have shown that CFT is able to identify a focus of bone infection and distinguish between septic and aseptic inflammation.…”

mentioning

confidence: 99%

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Ferreira¹,

Boratto²,

Cardoso³

et al. 2015

IJN

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Osteomyelitis is a progressive destruction of bones caused by microorganisms. Inadequate or absent treatment increases the risk of bone growth inhibition, fractures, and sepsis. Among the diagnostic techniques, functional images are the most sensitive in detecting osteomyelitis in its early stages. However, these techniques do not have adequate specificity. By contrast, radiolabeled antibiotics could improve selectivity, since they are specifically recognized by the bacteria. The incorporation of these radiopharmaceuticals in drug-delivery systems with high affinity for bones could improve the overall uptake. In this work, long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime were prepared and their ability to identify infectious foci (osteomyelitis) in animal models was evaluated. The effect of the presence of PEGylated lipids and surface-attached alendronate was evaluated. The bone-targeted long-circulating liposomal 99m technetium–ceftizoxime showed higher uptake in regions of septic inflammation than did the non-long-circulating and/or alendronate-non-coated liposomes, showing that both the presence of PEGylated lipids and alendronate coating are important to optimize the bone targeting. Scintigraphic images of septic or aseptic inflammation-bearing Wistar rats, as well as healthy rats, were acquired at different time intervals after the intravenous administration of these liposomes. The target-to-non-target ratio proved to be significantly higher in the osteomyelitis-bearing animals for all investigated time intervals. Biodistribution studies were also performed after the intravenous administration of the formulation in osteomyelitis-bearing animals. A significant amount of liposomes were taken up by the organs of the mononuclear phagocyte system (liver and spleen). Intense renal excretion was also observed during the entire experiment period. Moreover, the liposome uptake by the infectious focus was significantly high. These results show that long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime have a tropism for infectious foci.

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confidence: 99%

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Ferreira¹,

Boratto²,

Cardoso³

et al. 2015

IJN

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“…The advantage of this technique is the differentiation between bacterial and viral infections, or inflammation due to infection with microbial pathogens and inflammation due to immune response where no microbial invasion is present, as is the case, for example, in autoimmune disorders (Wareham et al 2005). This is achieved through the radiolabelling of antibiotics or other modalities and their subsequent use as imaging agents.…”

Section: Aptamers In the Detection Of Rickettsias Sensu Latomentioning

confidence: 99%

Aptamers in the Diagnosis of Rickettsiosis Sensu Lato

Godoy¹,

Missailidis²,

Favacho³

et al. 2014

VR&R

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Aptamers are short synthetic oligonucleotides that specifi cally bind to various molecular targets with high affi nity and selectivity. Aptamers have found two main applications in bacteriology, diagnosis in various sensing applications and riboswitches. Rickettsiosis are diseases caused by rickettsias 'sensu lato' including spotted fever, typhus, anaplasmosis, ehrlichiosis, bartonellosis and Q fever. Traditional methodologies for the diagnosis of diseases associated with rickettsias 'sensu lato' are based on serological testing, bacterial cultures and molecular assays. However, an increasing number of novel technologies, including aptamer-based diagnostic sensors, are now on the horizon, opening up possibilities for earlier diagnosis and more sensitive assays. Th is perspective looks at the contribution of aptamers to rickettsias 'sensu lato' diagnosis, providing information on the 'state of the art' in this emerging fi eld..

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“…Nuclear medicine has ideal techniques to visualize inflammatory processes using noninvasive methods of whole-body scanning, enabling the determination of both the localization and the number of inflammatory foci. On the other hand, nuclear medicine imaging ensures discrimination of infections from inflammation because of technical sensitivity [1,4,5].…”

Section: Introductionmentioning

confidence: 99%

“…This is important because the infection may be acute or chronic, body location, tissue type and patient's immune status. Hence the development of infectionspecific new radiolabeled antibiotics has also been studied [4,25].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of 99mTc-labeled antibiotics for infection detection

Yurt

2010

Ann Nucl Med

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One of the fields of research in nuclear medicine is the development of new radiopharmaceuticals for imaging infection and inflammation in humans. For this development, several antimicrobial peptides, antibiotics, antibiotic peptide and chemotactic peptides, etc., have been radiolabeled with different radionuclides (⁶⁷Ga, ⁹⁹(m)Tc, ¹¹¹In, ¹⁸F, ¹³¹I, etc.) and their imaging potentials studied. Actually, it is very important to distinguish between infection and inflammation. In this respect, radiolabeled antibiotics have advantages because many of the properties of the ideal infection-specific agent through antibiotics localizes in infection site. In this review, only ⁹⁹(m)Tc-labeled antibiotics are evaluated and discussed.

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Advances in bacterial specific imaging

Cited by 28 publications

References 15 publications

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Aptamers in the Diagnosis of Rickettsiosis Sensu Lato

Evaluation of 99mTc-labeled antibiotics for infection detection

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