Isolation and partial characterization of a D-galactose-binding lectin from the latex of Synadenium carinatum

Souza, Maria Aparecida de; Amâncio-Pereira, Francielle; Cardoso, Cássia Regina Primila; Silva, Adriano Gomes da; Silva, Edmar Gomes; Andrade, Lívia Resende; Peña, J; Lanza, Henrique; Afonso-Cardoso, Sandra R.

doi:10.1590/s1516-89132005000600005

Cited by 21 publications

(28 citation statements)

References 33 publications

(35 reference statements)

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“…Trabalhos demonstram que a S. grantii possui glicoproteínas complexas, denominadas lectinas, encontradas principalmente no látex, que são potentes aglutininas para eritrócitos humanos (Uzabakiliho et al, 1987;Mrinalini, 2002;Rajesh et al, 2006;Souza et al, 2005). Assim, a presença das lectinas pode influenciar a migração de leucócitos e o acúmulo de sangue nos vasos sanguíneos dos órgãos mais irrigados, culminando nos efeitos histopatológicos supracitados na toxicidade aguda do látex e na toxicidade subaguda das folhas.…”

Section: Tabela 3 Hábitos Fisiológicos Diários De Ratas Tratadas Comunclassified

Avaliação da toxicidade aguda e subaguda, em ratos, do extrato etanólico das folhas e do látex de Synadenium umbellatum Pax.

Cunha¹,

Azeredo²,

Mendonça³

et al. 2009

Rev. bras. farmacogn.

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RESUMO:O uso de plantas medicinais tem sido muito significativo nos últimos anos, sendo incentivado pela Organização Mundial de Saúde (OMS). Synadenium umbellatum Pax, Euphorbiacea (vulgo cola-nota, cancerola, milagrosa) tem o látex usado empiricamente como antitumoral e antiinflamatório. Por existir espécies tóxicas nesta família e visando à segurança no uso de extratos vegetais, tal estudo avaliou a toxicidade pré-clínica do látex e do extrato etanólico das folhas (EEF) de S. umbellatum, por via oral, em ratas Wistar. O estudo seguiu diretrizes do Guideline 423 (toxicidade aguda) e Guideline 407 (toxicidade subaguda) da OECD (Organisation for Economic Cooperation and Development). Na toxicidade aguda do látex e do EEF, não se observou letalidade nem alterações fisiológicas e comportamentais das ratas na dose de 2000 mg/ kg, sendo praticamente atóxico. Porém, na análise histopatológica, o látex ocasionou congestão e infiltrado leucocitário nos rins, fígado e pulmões, efeitos não observados com o EEF. Na toxicidade subaguda, doses de 50, 100 e 200 mg/kg de EEF não produziram alterações dose-dependentes significativas nos parâmetros laboratoriais e fisiológicos, nem alterações macroscópicas e histopatológicas nos órgãos das ratas. Contudo, o uso crônico da planta S. umbellatum merece mais estudos.Unitermos: Synadenium umbellatum, toxicidade aguda, toxicidade subaguda, toxicidade pré-clinica.ABSTRACT: "Acute and subacute toxicity studies of the latex and of the ethanolic extract of the leaves of Synadenium umbellatum Pax in rats". The use of medicinal plants has been being very significant in the last years, being the use encouraged by WHO. Synadenium umbellatum Pax, Euphorbiacea (popularly known as cola-note, cancerola, miraculous) has the latex used empirically as anti-cancerous and anti-inflammatory. For there being toxic species in this family and aiming at the safety in the use of vegetable extracts, such study evaluated the pre-clinical toxicity of the latex and of the ethanolic extract of the leaves (EEL) of S. umbellatum, administrated by oral route, in Wistar female rats. The study followed OECD's Guidelines for test of acute toxicity (Guideline 423) and for subacute toxicity (Guideline 407). In the acute toxicity of latex and EEL, behavioral and physiological alterations were not observed neither animal's death in the dose level of 2,000 mg/kg. However, the latex caused congestion and leukocytes infiltration of the kidneys, liver and lungs, effects not observed with EEL. In the subacute toxicity, dose levels of 50, 100 and 200 mg/kg of EEL did not produced significant dose-dependent alterations in the lab results and no physiologic, macroscopic and hystopathological alterations. EEL of S. umbellatum is practically poisonless in acute exposure; already the latex can cause hystological damages. The chronic use of S. umbellatum needs more specific studies.

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Section: Tabela 3 Hábitos Fisiológicos Diários De Ratas Tratadas Comunclassified

Avaliação da toxicidade aguda e subaguda, em ratos, do extrato etanólico das folhas e do látex de Synadenium umbellatum Pax.

Cunha¹,

Azeredo²,

Mendonça³

et al. 2009

Rev. bras. farmacogn.

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“…Cunha et al (2009) also evaluated the toxicity of Synadenium. Species belonging to this genus are widely used in folk medicine to treat several diseases like cancer, peptic ulcers and other health problems (Ortêncio, 1997;Souza et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Anti-ulcer activity of Synadenium grantii latex

Costa¹,

David²,

Pinto³

et al. 2012

Rev. bras. farmacogn.

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Synadenium grantii Hook f., Euphorbiaceae, is popularly known as leitosinha or janaúba. The diluted latex (18 drops/L of water) is commonly used in the south of Brazil to treat gastric disturbances. This study evaluated phytochemical screening and toxicity using Artemia salina Leach of crude bark extract and also latex. The toxicity and the anti-ulcer activity of S. grantii latex were also tested in rats. Phytochemical results showed presence of tannins, terpenes, unsaponificable substances, coumarins and anthraquinones in the crude bark extract and terpenes in the latex. Gas chromatography-mass spectrometry (GC-MS) analysis demonstrated the presence of diterpene tigliane esters in the latex, identified as 12-deoxyphorbol-13-(2-metilpropionate) and phorbol 12,13,20-triacetate. The toxicity results using A. salina presented CL50 26.58μg/mL and CL50 778.66μg/mL, for the latex and the crude bark extract respectively. The toxicological hepatic parameters of the diluted latex were not different to the control group (p<0.05). The eosinophils cells showed an increase in both the diluted and pure latex groups. The pure latex showed gastric protection of 90% (p<0.05) and the diluted latex showed 6% compared to the negative control. Therefore, our data indicate that S. grantii latex, under research conditions presented gastric protection. Pure latex showed more toxicity than the diluted latex.

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“…Lectin specificity is usually defined according to the mono-or oligosaccharide that is able to inhibit the agglutinating activity induced by the lectin (Souza et al, 2005). Lectins are able to recognize and binding specifically carbohydrate and glycoconjugates.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

Braga

Sá

Ribeiro

et al. 2011

Braz. arch. biol. technol.

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Isolation and partial characterization of a D-galactose-binding lectin from the latex of Synadenium carinatum

Cited by 21 publications

References 33 publications

Avaliação da toxicidade aguda e subaguda, em ratos, do extrato etanólico das folhas e do látex de Synadenium umbellatum Pax.

Avaliação da toxicidade aguda e subaguda, em ratos, do extrato etanólico das folhas e do látex de Synadenium umbellatum Pax.

Anti-ulcer activity of Synadenium grantii latex

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

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