Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

Goulart, Antônio José; Carmona, Eleonora Cano

doi:10.1590/s1516-89132005000300001

Cited by 32 publications

(12 citation statements)

References 31 publications

(31 reference statements)

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“…41 Goulart et al cultivated Rhizopus stolonifer on wheat bran to produce cellulase free xylanase, and the purified xylanase exhibited optimum pH and temperature at 6.0 and 45°C, respectively. 42 Our findings regarding the effect of metal ions on enzyme activity are in close agreement with other findings using Aspergillus caespitosus, in which Cu 2 + , Zn 2 + , and Ca 2 + inhibited the activity of xylanase and Mg 2 + , Al 3 + , and Ba 2 + greatly enhanced enzyme activity. Using Aspergillus fumigatus FBSPE-05, it was reported that Mn 2 + and Zn 2 + inhibited enzyme activity, with slight inhibition in the presence of Ca 2 + and K + .…”

Section: Fungal Xylanase Production From Wastessupporting

confidence: 92%

Production and Application of Thermostable Cellulase-Free Xylanase byAspergillus fumigatusfrom Agricultural Wastes

Abdel-Monem

El-Baz

Shetaia

et al. 2012

Industrial Biotechnology

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Xylan is a principal structural component in plant cell walls and could interfere in many industries and biological activities. Biosynthesis of the xylan-degrading enzyme xylanase by fungal isolates was investigated, using agricultural wastes as substrates for production. Aspergillus fumigatus was shown to be the superior isolate to produce xylanase, and wheat bran proved to be the most appropriate substrate for enzyme production and activity. Media composition and growth conditions were optimized for the production of fungal xylanase using Plackett-Burman multifactorial mixture design and Box-Behnken experimental design. Characterization of the purified xylanase revealed that the most favorable conditions for the maximum enzyme activity (57 IU/mL) were temperature of 55-60°C with thermal stability for 30 min and pH of 9 with enzyme stability for 20 min. Application of xylanase for biodegradation of sugarcane pulp fibers was examined using scanning electron micrographs, which exhibited a vigorous alteration in the topography of fiber surface and structure with a prolonged enzymatic treatment period.

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Section: Fungal Xylanase Production From Wastessupporting

confidence: 92%

Production and Application of Thermostable Cellulase-Free Xylanase byAspergillus fumigatusfrom Agricultural Wastes

Abdel-Monem

El-Baz

Shetaia

et al. 2012

Industrial Biotechnology

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“…Composed mainly of D-xylose, xylan is the most common hemicellulose and it is the second most abundant biopolymer found in nature (Terrasan et al, 2010). Xylanase is the major component of a group of enzymes, and acts by depolymerizing the xylan molecules in to monomers (Goulart et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

Reis

Ritter

Fontana

et al. 2015

Braz. J. Chem. Eng.

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-Penicillium echinulatum S1M29 is a mutant with cellulase and xylanase production comparable to the most studied microorganisms in the literature. However, its potential to produce these enzymes has not been fully investigated. This study aimed at optimizing salt and urea concentrations in the mineral solution, employing the response surface methodology. A 2 5-1 Fractional Factorial Design and a 2 3 Central Composite Design were applied to elucidate the effect of salts and urea in enzyme production. Lower concentrations of KH 2 PO 4 (2.0 g.L -1 ), (NH 4 ) 2 SO 4 (1.4 g.L -1 ), MgSO 4 .7H 2 O (0.375 g.L -1 ) and CaCl 2 (0.375 g.L -1 ) were most suitable for the production of all enzymes evaluated. Nevertheless, higher concentrations of urea (0.525 g.L -1 ) gave the best results for cellulase and xylanase production. The maximum FPase (1,5 U.m.L -1 ), endoglucanase (7,2 U.m.L -1 ), xylanase (30,5 U.m.L -1 ) and β-glucosidase (4,0 U.m.L -1 ) activities obtained with the planned medium were, respectively, 87, 16, 17 and 21% higher when compared to standard medium. The experimental design contributed to adjust the concentrations of minerals and urea of the culture media for cellulase and xylanase production by P. echinulatum, avoiding waste of components in the medium.

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“…Aspergillus terreus CECT 2663 produced α-L-rhamnosidase in SSF system [21]. Cellulose-free alkaline xylanase was produced by Rhizophus stolonifer in SSF system using wheat bran as a support [19]. Limited amount of studies are available for Rhizophus stolonifer in SSF system and this study involves in the application of SSF using paddy husk as support to produce naringinase enzyme from Rhizophus stolonifer.…”

Section: Discussionmentioning

confidence: 99%

Optimization of naringinase production by Rhizophus stolonifer in solid state fermentation media using paddy husk as support

Karuppaija

Ranganathan²,

Seevaratnam³

2016

SAJB

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Abstract:The palmyrah (Borassus flabellifer L) tree represents a major socio economic factor in tropics. The economic value of the tree is reduced by fruit pulp, due to the bitter compound present in the fruit pulp but it can be hydrolyzed by the usage of naringinase enzyme. Hence this enzyme plays a key role in the food industry and pharmaceuticals for its debittering nature. Some microbes like bacteria and fungiare involved in the natural production of cost effective naringinase. The aim of this work was to optimize the cultivation conditions and the media to produce naringinase by solid state fermentation of Rhizophus stolonifer, using paddy husk as the support. The highest enzyme activity was obtained on the 7 th day of incubation. Highest naringinase enzyme activity (57.97U/g DM) was obtained on the 7 th day of incubation and after that activity started to decline. The enzyme activity (212.885U/g DM) obtained with 0.75% naringin concentration was significantly higher than that of other naringin concentrations tested. When the regular medium (0.5% glucose) was replaced with (w/v) 1.5% glucose and 1.5% sucrose, the enzyme activity obtained with 1.5% of glucose (484.96U/g DM) was significantly higher than that of 1.5% sucrose (238.88U/g DM). When naringinase activity was tested with different glucose concentrations, activity obtained with 1.5% of glucose concentration was significantly higher than that of other concentrations. Among the nitrogen sources tested, when 0.5 % ammonium nitrate was used significantly higher naringinase was produced by Rhizophus stolonifer than any other sources and their concentrations. The cost to produce 1073.97U/g DM was reduced by 20.15% when conditions and media composition are optimized when compared to the basal medium(57.97U/g DM). Therefore when naringinase was produced by Rhizophus stolonifer under optimized condition, the production cost was marginally reduced.

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Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation

Cited by 32 publications

References 31 publications

Production and Application of Thermostable Cellulase-Free Xylanase byAspergillus fumigatusfrom Agricultural Wastes

Production and Application of Thermostable Cellulase-Free Xylanase byAspergillus fumigatusfrom Agricultural Wastes

STATISTICAL OPTIMIZATION OF MINERAL SALT AND UREA CONCENTRATION FOR CELLULASE AND XYLANASE PRODUCTION BY Penicillium echinulatum IN SUBMERGED FERMENTATION

Optimization of naringinase production by Rhizophus stolonifer in solid state fermentation media using paddy husk as support

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