“…This sample showed low level of contamination by unknown fuel, possibly due to leaks in the pipes and ground infiltrations. Until performing the biodegradation experiments, samples were stored at 5 o C. In order to verify the influence of the bioaugmentation technique in the biodegradation of diesel oil, batch biodegradation experiments (48 days) were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO 2 production (Bartha and Pramer, 1965;Régis and Bidoia, 2005;Inazaki et al, 2004;Mariano et al, 2007a). Mineralization studies involving measurements of total CO 2 production can provide excellent information on the biodegradability potential of hydrocarbons (Balba et al, 1998).…”