Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae

Kava-Cordeiro, V.; Queiroz, Marisa Vieira de; Pizzirani-Kleiner, Aline Aparecida; Azevedo, João Lúcio

doi:10.1590/s1516-89132005000100001

Cited by 3 publications

(3 citation statements)

References 23 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In some species, the diploid phase is considered transient, usually not identified, and the recombinants can be obtained directly from the heterokaryon. This process was called parameiosis (Bonatelli Jr et al, 1983;Bagagli et al, 1991) and have been observed in many fungal species, including Fusarium oxysporum (Molnar et al, 1990), Metarhizium anisopliae (Bagagli et al, 1991;Kava-Cordeiro et al, 2005), Cladosporium fulvum (Arnau and Oliver, 1993), Colletotrichum gloesporioides (Chacko et al, 1994), Beauveria bassiana (Paccola-Meirelles and Azevedo, 1991;Bello and Paccola-Meirelles, 1998). The results presented here showed that the oval conidia were efficient in obtaining the heterokaryon in C. sublineolum, representing an additional tool for genetic studies, mainly in strains incapable of producing falcate conidia.…”

Section: Resultsmentioning

confidence: 99%

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Nunes

Souza-Paccola

Andrade-Nobrega

et al. 2010

Braz. arch. biol. technol.

View full text Add to dashboard Cite

The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.

show abstract

Section: Resultsmentioning

confidence: 99%

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Nunes

Souza-Paccola

Andrade-Nobrega

et al. 2010

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Plasmid DNA was quantified using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific). The number of plasmid copies was adjusted to 6 Â 10 4 ll À1 , which corresponds to the number of genome copies in 2 ng genomic DNA of M. brunneum ARSEF 1066, assuming a genome size of 29.7 Mb as reported for M. anisopliae (Kava-Cordeiro et al, 2005). From this stock, 10-fold serial dilutions were prepared containing 6 Â 10 4 to 6 copies ll À1 .…”

Section: Real-time Pcr Quantificationmentioning

confidence: 99%

A PCR-based tool for cultivation-independent detection and quantification of Metarhizium clade 1

Schneider

Rehner

Widmer

et al. 2011

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

“…anisopliae, isolated from Deois flavopicta (Hemiptera:Cercopidae), obtained from a stock collection of the Department of Genetics and Evolution, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil. This strain is largely used in bioassays and genetic studies (9,11,20,23,32). The conidia used in these studies were producted on complete culture medium (CM) consisting of yeast extract, glucose, minerals, agar, and water, according to Alves et, 1998 (3).…”

Section: Strain Of M Anisopliaementioning

confidence: 99%

Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae)

Júnior¹,

Lacava²,

Messias³

et al. 2008

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

Citrus Variegated Chlorosis (CVC) is an economically important, destructive disease in Brazil and is caused by Xylella fastidiosa and transmitted by sharpshooter insects. In this study, the efficacy of the fungus Metarhizium anisopliae in controlling the sharpshooter Oncometopia facialis was studied by bioassay conditions. In the bioassay, insects were sprayed with a suspension containing 5 X 10 7 conidia mL -1. Adults captured in the field were treated in groups of 10 in a total of 11 replications per treatment. Significant differences between the natural mortality and the mortality of insects treated with the fungus were observed 6 days after inoculations (P<0.05). These significant differences increased until 10 days after treatment. The fungus caused 87.1% mortality, with the LT50 varying from 5 to 6 days. The LC50 was 1.2 X 10 6 conidia mL -1 , varying from 7.7 X 10 5 to 2 X 10 6 conidia mL -1 . The results showed that the sharpshooter O. facialis was susceptible to the entomopathogenic action of M. anisopliae in controlled condition during bioassay.

show abstract

Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae

Abstract: ABSTRACT

Cited by 3 publications

References 23 publications

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants

A PCR-based tool for cultivation-independent detection and quantification of Metarhizium clade 1

Bioassay assessment of metarhizium anisopliae (metchnikoff) sorokin (deuteromycota: hyphomycetes) against Oncometopia facialis (signoret) (hemiptera: cicadellidae)

Contact Info

Product

Resources

About