Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

Martín, N Betty San; Souza, Simone Regina de; Silva, Roberto da; Gomes, Eleni

doi:10.1590/s1516-89132004000500018

Cited by 101 publications

(73 citation statements)

References 27 publications

(22 reference statements)

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“…• C (Foda et al 1984;Vazquez et al 1993;Martin et al 2004). The purified PG from R. oryzae MTCC 1987 does not loose its activity appreciably up to 30…”

Section: )mentioning

confidence: 99%

Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

et al. 2012

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An acidic polygalacturonase (PG) secreted by Rhizopus oryzae MTCC-1987 in submerged fermentation condition has been purified to electrophoretic homogeneity using ammonium sulphate fractionation and anion exchange chromatography on diethylaminoethyl cellulose. The purified enzyme gave a single protein band in sodium dodecyl sulphatepolyacrylamide gel electrophoresis analysis with a molecular mass corresponding to 75.5 kDa. The Km and kcat values of the PG were 2.7 mg/mL and 2.23 × 10 3 s −1 , respectively, using citrus polygalacturonic acid as the substrate. The optimum pH of the purified PG was 5.0 and it does not loose activity appreciably if left for 24 hours in the pH range from 5.0 to 12.0. The optimum temperature of purified enzyme was 50• C and the enzyme does not loose activity below 30• C if exposed for two hours. The purified enzyme showed complete inhibition with 1 mM Ag + , Hg 2+ and KMnO4, while it was stimulated to some extent by Co 2+ . The purified PG exhibited retting of Crotalaria juncea fibre in absence of ethylenediaminetetraacetic acid.

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“…• C (Foda et al 1984;Vazquez et al 1993;Martin et al 2004). The purified PG from R. oryzae MTCC 1987 does not loose its activity appreciably up to 30…”

Section: )mentioning

confidence: 99%

Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

et al. 2012

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“…In contrast, PG production from Penicillum sp. EGC5 was reported maximum at 8 days using a mixture of substrates (16).…”

Section: Discussionmentioning

confidence: 97%

“…This could be due to loss of moisture with prolonged incubation at 50°C and/or interaction with other components in the culture medium (9). In most cases, the optimum incubation period for PG production in SSF varied from 3 to 6 days, depending on the environmental conditions (4,16). In contrast, PG production from Penicillum sp.…”

Section: Discussionmentioning

confidence: 99%

Exo-polygalacturonase production by Bacillus subtilis CM5 in solid state fermentation using cassava bagasse

Swain¹,

Kar²,

Ray³

2009

Braz. J. Microbiol.

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The purpose of this investigation was to study the effect of Bacillus subtilis CM5 in solid state fermentation using cassava bagasse for production of exo-polygalacturonase (exo-PG). Response surface methodology was used to evaluate the effect of four main variables, i.e. incubation period, initial medium pH, moisture holding capacity (MHC) and incubation temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected exo-PG production. The experimental results showed that the optimum incubation period, pH, MHC and temperature were 6 days, 7.0, 70% and 50°C, respectively for optimum exo-PG production.

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“…The agro industrial residues used for the enzyme production represent an economic alternative for the carbon sources and also contribute to decrease the pollution concern about waste accumulation in the environment, therefore they have been frequently studied (Martin et al, 2004). Lemon peel was an interesting carbon source for PG production.…”

Section: Discussionmentioning

confidence: 99%

Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis

Damásio

Maller

Silva

et al. 2011

Braz. arch. biol. technol.

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Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

Abstract: 4.5-5.0 and 9.0 and 40°C, respectively.

Cited by 101 publications

References 27 publications

Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre

Exo-polygalacturonase production by Bacillus subtilis CM5 in solid state fermentation using cassava bagasse

Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis

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